Abbildung 1: Schematische Darstellung der IVP (modifiziert nach NIEMANN u.
MEINECKE 1993)
Abbildung 2: Schematische Darstellung physikalischer Prozesse beim Einfrieren von Zellen (mod. nach Mazur 1977)
Abbildung 3: Strukturformel Glyzerin Abbildung 4: Strukturformel Ethylenglycol Abbildung 5: Strukturformel Dimethylsulfoxid Abbildung 6: Strukturformel Propylenglycol
Abbildung 7: Strukturformeln von Saccharose (links) und Trehalose (rechts) Abbildung 8: Strukturformeln von Polyvinylpyrrolidon (PVP, links) und
Polyvinylalkohol (PVA, rechts)
Abbildung 9: Schematische Darstellung des Elektronenmikroskop-Kupferrasters (EMG)
Abbildung 10: OPS-Methode nach VJATA et al. 1998
Abbildung 11: Schematische Darstellung des Cryoloop nach LANE et al. 1999b Abbildung 12: Schematische Darstellung eines One-Step-Verfahrens für die
Vitrifikation von Embryonen Abbildung 13: Zeitachse der In-vitro-Produktion.
Abbildung 14: KOK der Kategorien 1-3 (*:1, **:2, ***:3)
Abbildung 15: Vitriplug; (*: Ansicht von der Seite, **: Ansicht von oben) Abbildung 16: Ethidium homodimer
Abbildung 17: Hoechst 33342
Abbildung 18: Schematischer Überblick über den Versuchsaufbau Abbildung 19: Reexpansions- und Schlupfraten der Embryonen nach
Vitrifikation (V1,V2) und Kontrolle [(KV1, KV2);(MW ± STABW:
a:b:c P≤ 0,05)]
Abbildung 20: Gesamtzellzahl und Anzahl toter Zellen der Blastozysten der verschiedenen Versuchsgruppen
Abbildung 21: Lebend-Tot-Zellratio in den verschiedenen Versuchsgruppen (MW ± SEM: a:b P≤ 0,05)
Abbildung 22: Geschlüpfte Blastozysten nach Zellfärbung mittels Ethidium homodimer und Hoechst-33342 der Gruppen a) V1, b) KV1, c) V2, d) KV2 und e) K
Abbildung 23: Relative Transkriptmenge von SLC2A1 in präimplantatorischen Embryonen der verschiedenen Versuchsgruppen (MW ± SEM:
a:b < 0,05)
Abbildung 24: Relative Transkriptmenge von TJP1 in präimplantatorischen Embryonen der verschiedenen Versuchsgruppen (MW ± SEM:
P a:b < 0,05, P * < 0,1)
Abbildung 25: Relative Transkriptmenge von IFNT2 in präimplantatorischen Embryonen der verschiedenen Versuchsgruppen
Abbildung 26: Relative Transkriptmenge von HSPA1A in präimplantatorischen Embryonen der verschiedenen Versuchsgruppen (MW ± SEM:
P a:b:c < 0,05)
Abbildung 27: Relative Transkriptmenge von DSC2 in präimplantatorischen Embryonen der verschiedenen Versuchsgruppen (MW ± SEM:
P a:b < 0,05)
Abbildung 28: Relative Transkriptmenge von PTGS2 in präimplantatorischen Embryonen der verschiedenen Versuchsgruppen
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