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Mein ganz herzlicher Dank gilt PD Dr. Johannes Schenkel ohne dessen unermüdliche Unterstützung und Betreuung diese Dissertation nicht durchführbar gewesen wäre. Vielen Dank, dass Du immer ein offenes Ohr für Fragen und Probleme hattest, die wir zusammen besprochen und gelöst haben, sowie für die vielen Stunden des Korrekturlesens!
Meinen besonderen Dank an Prof. Dr. Hansjoachim Hackbarth für die Überlassung des Themas und seine freundliche, hilfreiche Unterstützung bei der Anfertigung der Arbeit.
Dr. Heinrich Bürgers danke ich für die Einarbeitung in die Fluoreszenzmikroskopie und die Betreuung aller technischen Aspekte der Arbeit, insbesondere die telefonische Unterstützung bei auftretenden Problemen.
Bei Prof. Dr. Angel Alonso möchte ich mich für die gute Betreuung im Rahmen des PhD-Programms des DKFZ, an dem ich teilnehmen durfte, bedanken.
Den Mitarbeitern der Kryokonservierung des Deutschen Krebsforschungszentrums, Frau Andrea Rausch, Frau Beatrix Imkeit und Herr Helmut Eskerski, danke ich für die Hilfe bei der Durchführung meiner Experimente.
Außerdem möchte ich mich bei allen Mitarbeitern des Zentralen Tierlabors für ihre Unterstützung und gute Zusammenarbeit, trotz des erhöhten Arbeitsaufwandes durch die zusätzlichen Tiere, bedanken.
Ganz besonderes danke ich Dr. Werner Rittgen für die Beratung und Unterstützung bei der statistischen Analyse meiner Ergebnisse.
Vielen Dank an meine Familie, insbesondere meiner Mutter Nicole und Schwester Steffi, für die Unterstützung während der letzten zwei Jahre. Ohne Euch und die vielen aufbauenden Telefonate hätte ich das nicht geschafft! Danke, dass ihr jederzeit für mich da seid!
Euch habe ich mich in Heidelberg richtig wohl gefühlt und konnte mich immer wieder voller Elan meiner Dissertation widmen.
Ein ganz besonderer Dank geht an meine beste Freundin Ursu, die mir trotz eigener Dissertation und großer Entfernung immer zur Seite gestanden hat.
Vielen Dank auch an Sonja, die mir durch ihr Korrekturlesen eine große Hilfe war.
Mein Dank gilt dem Bundesministerium für Bildung und Forschung, die das Projekt
„Versuchstiersparende Qualitätskontrolle kryokonservierter Spermatozoen von Mausmutanten“ (Forschungsvorhaben 315152) und meine Stelle in diesem finanziert haben.
vorgestellt:
SCHENKEL J., AK. DIERCKS, H. BÜRGERS (2009):
Animal saving quality assessment of cryopreserved transgenic mouse spermatozoa.
47. Wissenschaftliche Tagung der Gesellschaft für Versuchstierkunde, Wien, 85.
SCHENKEL J., AK. DIERCKS, H. BÜRGERS, H.U. DODT (2009):
Reduction of animal experiments by the use of fluorescence, imaging and advanced analysis techniques.
47. Wissenschaftliche Tagung der Gesellschaft für Versuchstierkunde, Wien, 127.
DIERCKS AK., H. BÜRGERS, H. HACKBARTH, J. SCHENKEL (2010):
Subtractive staining of cryopreserved spermatozoa for quality assessment.
11th FELASA and 40th ScandLAS Joint Symposium, Helsinki, Finland, 52 DIERCKS AK., H. BÜRGERS, H. HACKBARTH, J. SCHENKEL (2010):
Quality assessment of cryopreserved spermatozoa by a subtractive staining technique.
Mouse Development, Genetics & Genomics, Cold Spring Harbor, USA, 87.