All units are abbreviated according to the International Unit System.
a: anti
aa: aminoacid a: ampere C‐: Carboxy‐Terminus
Cdk1: cyclin dependent kinase1
cDNA: complementary deoxyribonucleic acid CO2: carbon dioxid
DMSO: Dimethylsulfoxide
DAPI: 4´, 6‐diamidino‐2‐phenylindole DMEM: Dulbecco‘s Modified Eagle Serum DNA: deoxyribonucleic acid
dNTP: Desoxynucleotide DTT: dithiothreitol E. coli: Escherichia coli
ECL: enhanced chemiluminescence EDTA: ethylene‐dinitrilo‐tetraacetic acid EGTA: ethylene‐gycol‐tetraacetic acid engl.: english
FACS: fluorescence activated cell sorter FCS: fetal calf serum
fl full‐lenght
GFP: green fluorescent protein gl2 GL2‐Luciferase‐siRNA
GMPCPP: guanylyl‐(α, β)‐methylene‐diphosphonate GST: glutathione S‐transferase
GTP: guanosine triphosphate IF: immunofluorescence h: hour
HCl: hydrochloric acid
Hepes: N‐2‐Hydroxyethypiperazine‐N’‐2‐ethane sulfonic acid
IgG: Immunoglobulin G
IP: immunoprecipitation
IPTG: isopropyl‐beta‐D‐thiogalactopyranoside k: kinetochore
kb: kilobasepairs KCl: potassiumchlorid kDa: kilo Dalton
Kif: Kinesin superfamily protein Kif18A: Kinesin‐family member 18A l: Liter
LB: Luria Bertani M: milli
M: Molar min: minute(s)
Mps1: monopolar spindle protein 1 MT: microtubule
MW: molecular weight μ: micro
n: nano
NaCl: sodiumchloride Noc: nocodazole OA: ocadaic acid OD: optical density ORF: open reading frame
PAGE: Polyarcylamid‐gel‐elektrophoresis PBS: Phosphate‐buffered saline
PCR: Polymerase chain reaction
PIPES: 1,4‐Piperazinediethanesulfonic acid Plk1: Polo‐like kinase 1
PMSF: phenylmethylsulfonyl fluoride RNA: Ribonucleidacid
RNAi: small interference ribonucleic acid rpm: rounds per minute
RT: room temperature S674: serine at position 674
SAC: spindle assembly checkpoint SAP: Shrimp alkaline phosphatase
SDS‐PAGE: Sodium dodecylsulfate polyacrylamid gelelectrophoresis
siRNA: short interfering RNA TEMED: Tetramethylethylendiamine U: Units
V: Volt
IB: immunoblotting
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Acknowledgements
I would like to express my deep gratitude to my supervisor Prof. Thomas U. Mayer for giving me the opportunity to work on the fascinating and exiting Kif18A project.
He was the one who taught me patiently the different techniques and introduced me into the field of mitosis and motorproteins, always open to discuss, explain and help, thereby greatly influencing my current way of performing experiments and approaching problems. His wide knowledge and his logical way of thinking have been of great value for me. He gave me an excellent feeling in the lab by choosing again and again people who with respect to their characters fit well in the lab thereby creating a wonderful atmosphere in the lab with respect to both working and personal relationships.
I am grateful to Prof. Martin Scheffner and Prof. Christof Hauck for agreeing to be the second referees for my thesis and for their time.
I would like to thank my labmembers Johanna, Stefan, Julia, Eva, Thommy, Mario, Anna, Martina, Lucia and the former labmembers Hümmelmann, Jenny, Andi und Nadine for fruitful discussions and for making me laugh everyday. I really enjoyed working with you and it was always a big pleasure to be with you.
I am grateful to Lucia for her friendship and the various non‐lab activities, making me discover Konstanz and to enjoy life outside the lab. I deeply appreciated and enjoyed the time we spent together.
I am grateful to Evelyn for joining me at the walks around the MPI with Packa and Taylor, for her “Brotzeit” in the late evenings and her computer help.
I wish to thank my friends in Munich, for their endurance through all the years of friendship even though I had barely time to meet them.
My deepest gratitude goes to Mütterchensus, Papa und Berni, for their enduring support, for beliving in me and for their patience over the last years.
I owe my loving thanks to my friend Stefan, for his encouragement and
I owe my loving thanks to my friend Stefan, for his encouragement and