8. Zellbiologische Methoden
8.7 Anzuchtsbedingungen für A. thaliana
A. thaliana wurde im Gewächshaus angezogen (Tab. 22), wobei zur Bildung von Blattrosetten die Pflanzen im Kurztag angezogen wurden, während zur Samenbildung die Pflanzen im Langtag standen. Es wurden A. thaliana Ecotyp Columbia (Col-0), ∆deg7-Mutanten und transgene A. thalina (Tab. 23) angezogen.
Tab. 22: Anzuchtsbedingungen im Gewächshaus
Kurztag Langtag
Lichtperiode 9 h / 21°C 16 h / 23°C Dunkelperiode 15 h / 20°C 8 h / 17°C Photonenflussdichte 100-120 µmol m-2 s-1 150 µmol m-2 s-1
Luftfeuchtigkeit 60% 40%
Tab. 23: Verwendete transgene A. thaliana
Name Protein
pPH49 Überexpression von DEG7α-GFP pPH56 Überexpression von DEG2-GFP pCH06 Überexpression von DEG7β-GFP pUM16 Überexpression VirD2-GFP
pUM18 Überexpression DEG7Met1-Leu243-GFP pUM26 Überexpression FtsH11Met1-Gln245-GFP
Referenzen
Abbas, H. K., T. Tanaka, S. O. Duke, J. K. Porter, E. M. Wray, L. Hodges, A. E. Sessions, E.
Wang, A. H. Merrill, Jr. and R. T. Riley (1994). "Fumonisin- and AAL-Toxin-Induced Disruption of Sphingolipid Metabolism with Accumulation of Free Sphingoid Bases." Plant Physiol 106(3): 1085-1093.
Adam, Z., I. Adamska, K. Nakabayashi, O. Ostersetzer, K. Haussuhl, A. Manuell, B. Zheng, O.
Vallon, S. R. Rodermel, K. Shinozaki and A. K. Clarke (2001). "Chloroplast and mitochondrial proteases in Arabidopsis. A proposed nomenclature." Plant Physiol 125(4): 1912-1918.
Adam, Z., A. Rudella and K. J. van Wijk (2006). "Recent advances in the study of Clp, FtsH and other proteases located in chloroplasts." Curr Opin Plant Biol 9(3): 234-240.
AGI (2000). "Analysis of the genome sequence of the flowering plant Arabidopsis thaliana."
Nature 408: 796-815.
Alba, B. M. and C. A. Gross (2004). "Regulation of the Escherichia coli sigma-dependent envelope stress response." Mol Microbiol 52(3): 613-619.
Alonso, J. M., A. N. Stepanova, T. J. Leisse, C. J. Kim, H. Chen, P. Shinn, D. K. Stevenson, J.
Zimmerman, P. Barajas, R. Cheuk, C. Gadrinab, C. Heller, A. Jeske, E. Koesema, C. C. Meyers, H. Parker, L. Prednis, Y. Ansari, N. Choy, H. Deen, M. Geralt, N. Hazari, E. Hom, M. Karnes, C. Mulholland, R. Ndubaku, I. Schmidt, P. Guzman, L. Aguilar-Henonin, M. Schmid, D.
Weigel, D. E. Carter, T. Marchand, E. Risseeuw, D. Brogden, A. Zeko, W. L. Crosby, C. C.
Berry and J. R. Ecker (2003). "Genome-wide insertional mutagenesis of Arabidopsis thaliana."
Science 301(5633): 653-657.
Arnsburg, K. and J. Kirstein-Miles (2014). "Interrelation between protein synthesis, proteostasis and life span." Curr Genomics 15(1): 66-75.
Baker, B. M. and C. M. Haynes (2011). "Mitochondrial protein quality control during biogenesis and aging." Trends Biochem Sci 36(5): 254-261.
Barchinger, S. E. and S. E. Ades (2013). "Regulated Proteolysis: Control of the Escherichia coli sigma(E)-Dependent Cell Envelope Stress Response." Subcell Biochem 66: 129-160.
Barrett, A. J., N. D. Rawlings and E. A. O'Brien (2001). "The MEROPS database as a protease information system." J Struct Biol 134(2-3): 95-102.
Geier and B. Fahrenkrog (2009). "Nuclear localisation is crucial for the proapoptotic activity of the HtrA-like serine protease Nma111p." J Cell Sci 122(Pt 21): 3931-3941.
Referenzen
98
Bennett, M. D., I. J. Leitch, H. J. Price and J. S. Johnston (2003). "Comparisons with Caenorhabditis (approximately 100 Mb) and Drosophila (approximately 175 Mb) using flow cytometry show genome size in Arabidopsis to be approximately 157 Mb and thus approximately 25% larger than the Arabidopsis genome initiative estimate of approximately 125 Mb." Ann Bot 91(5): 547-557.
Berg, J. M., J. L. Tymoczko and L. Stryer (2013). "Stryer Biochemie." Springer Spektrum 7.
Auflage.
Bozkurt, T. O., S. Schornack, J. Win, T. Shindo, M. Ilyas, R. Oliva, L. M. Cano, A. M. Jones, E. Huitema, R. A. van der Hoorn and S. Kamoun (2011). "Phytophthora infestans effector AVRblb2 prevents secretion of a plant immune protease at the haustorial interface." Proc Natl Acad Sci U S A 108(51): 20832-20837.
Brown, L. A. and A. Baker (2003). "Peroxisome biogenesis and the role of protein import." J Cell Mol Med 7(4): 388-400.
Bui, K. H., A. von Appen, A. L. DiGuilio, A. Ori, L. Sparks, M. T. Mackmull, T. Bock, W.
Hagen, A. Andres-Pons, J. S. Glavy and M. Beck (2013). "Integrated structural analysis of the human nuclear pore complex scaffold." Cell 155(6): 1233-1243.
Bukau, B., J. Weissman and A. Horwich (2006). "Molecular chaperones and protein quality control." Cell 125(3): 443-451.
Carter, C., S. Pan, J. Zouhar, E. L. Avila, T. Girke and N. V. Raikhel (2004). "The vegetative vacuole proteome of Arabidopsis thaliana reveals predicted and unexpected proteins." Plant Cell 16(12): 3285-3303.
Chao, J. R., E. Parganas, K. Boyd, C. Y. Hong, J. T. Opferman and J. N. Ihle (2008). "Hax1-mediated processing of HtrA2 by Parl allows survival of lymphocytes and neurons." Nature 452(7183): 98-102.
Chassin, Y., E. Kapri-Pardes, G. Sinvany, T. Arad and Z. Adam (2002). "Expression and characterization of the thylakoid lumen protease DegP1 from Arabidopsis." Plant Physiol 130(2): 857-864.
Chelsky, D., R. Ralph and G. Jonak (1989). "Sequence requirements for synthetic peptide-mediated translocation to the nucleus." Mol Cell Biol 9(6): 2487-2492.
Chiba, A., H. Ishida, N. K. Nishizawa, A. Makino and T. Mae (2003). "Exclusion of ribulose-1,5-bisphosphate carboxylase/oxygenase from chloroplasts by specific bodies in naturally senescing leaves of wheat." Plant Cell Physiol 44(9): 914-921.
Clausen, T., M. Kaiser, R. Huber and M. Ehrmann (2011). "HTRA proteases: regulated proteolysis in protein quality control." Nat Rev Mol Cell Biol 12(3): 152-162.
Clausen, T., C. Southan and M. Ehrmann (2002). "The HtrA family of proteases: implications for protein composition and cell fate." Mol Cell 10(3): 443-455.
Clough, S. J. and A. F. Bent (1998). "Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana." Plant J 16(6): 735-743.
Dangl, J. L. and J. D. Jones (2001). "Plant pathogens and integrated defence responses to infection." Nature 411(6839): 826-833.
Referenzen DeMartino, G. N., R. J. Proske, C. R. Moomaw, A. A. Strong, X. Song, H. Hisamatsu, K.
Tanaka and C. A. Slaughter (1996). "Identification, purification, and characterization of a PA700-dependent activator of the proteasome." J Biol Chem 271(6): 3112-3118.
Dingwall, C., J. Robbins, S. M. Dilworth, B. Roberts and W. D. Richardson (1988). "The nucleoplasmin nuclear location sequence is larger and more complex than that of SV-40 large T antigen." J Cell Biol 107(3): 841-849.
Earley, K. W., J. R. Haag, O. Pontes, K. Opper, T. Juehne, K. Song and C. S. Pikaard (2006).
"Gateway-compatible vectors for plant functional genomics and proteomics." Plant J 45(4):
616-629.
Ehrmann, M. and T. Clausen (2004). "Proteolysis as a regulatory mechanism." Annu Rev Genet 38: 709-724.
Eleutherio, E. C., P. S. Araujo and A. D. Panek (1993). "Protective role of trehalose during heat stress in Saccharomyces cerevisiae." Cryobiology 30(6): 591-596.
Ellis, R. J. and A. P. Minton (2006). "Protein aggregation in crowded environments." Biol Chem 387(5): 485-497.
Erhardt, M. (2012). "DEG Proteases in Arabidopsis thaliana." Dissertation Universität Konstanz.
Erhardt, M., I. Adamska and O. L. Franco (2010). "Plant nuclear proteomics--inside the cell maestro." FEBS J 277(16): 3295-3307.
Fahrenkrog, B. (2011). "Nma111p, the pro-apoptotic HtrA-like nuclear serine protease in Saccharomyces cerevisiae: a short survey." Biochem Soc Trans 39(5): 1499-1501.
Fahrenkrog, B. and U. Aebi (2002). "The vertebrate nuclear pore complex: from structure to function." Results Probl Cell Differ 35: 25-48.
Fahrenkrog, B., U. Sauder and U. Aebi (2004). "The S. cerevisiae HtrA-like protein Nma111p is a nuclear serine protease that mediates yeast apoptosis." J Cell Sci 117(Pt 1): 115-126.
Fomicheva, A. S., A. I. Tuzhikov, R. E. Beloshistov, S. V. Trusova, R. A. Galiullina, L. V.
Mochalova, N. V. Chichkova and A. B. Vartapetian (2012). "Programmed cell death in plants."
Biochemistry (Mosc) 77(13): 1452-1464.
Funck, D., S. Eckard and G. Muller (2010). "Non-redundant functions of two proline dehydrogenase isoforms in Arabidopsis." BMC Plant Biol 10: 70.
Galetskiy, D., J. N. Lohscheider, A. S. Kononikhin, I. A. Popov, E. N. Nikolaev and I. Adamska (2011). "Mass spectrometric characterization of photooxidative protein modifications in Arabidopsis thaliana thylakoid membranes." Rapid Commun Mass Spectrom 25(1): 184-190.
Garcia-Lorenzo, M., A. Sjodin, S. Jansson and C. Funk (2006). "Protease gene families in Populus and Arabidopsis." BMC Plant Biol 6: 30.
Gasparic, K. (2014). Dissertation Universtität Konstanz (unveröffentlicht).
Giberti, S., D. Funck and G. Forlani (2014). "Delta -pyrroline-5-carboxylate reductase from Arabidopsis thaliana: stimulation or inhibition by chloride ions and feedback regulation by proline depend on whether NADPH or NADH acts as co-substrate." New Phytol.
Referenzen
100
Gietz, R. D. and R. A. Woods (2002). "Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method." Methods Enzymol 350: 87-96.
Gilchrist, D. G., H. Wang and R. M. Bostock (1995). "Sphingosine-related mycotoxins in platn and animal diseases." Can.J.Bot. 73: 459-467.
Glickman, M. H. and A. Ciechanover (2002). "The ubiquitin-proteasome proteolytic pathway:
destruction for the sake of construction." Physiol Rev 82(2): 373-428.
Gorlich, D., S. Kostka, R. Kraft, C. Dingwall, R. A. Laskey, E. Hartmann and S. Prehn (1995).
"Two different subunits of importin cooperate to recognize nuclear localization signals and bind them to the nuclear envelope." Curr Biol 5(4): 383-392.
Gorlich, D., S. Prehn, R. A. Laskey and E. Hartmann (1994). "Isolation of a protein that is essential for the first step of nuclear protein import." Cell 79(5): 767-778.
Gottesman, S. (2003). "Proteolysis in bacterial regulatory circuits." Annu Rev Cell Dev Biol 19:
565-587.
Graveley, B. R. (2000). "Sorting out the complexity of SR protein functions." RNA 6(9): 1197-1211.
Gu, C., M. Shabab, R. Strasser, P. J. Wolters, T. Shindo, M. Niemer, F. Kaschani, L. Mach and R. A. van der Hoorn (2012). "Post-translational regulation and trafficking of the granulin-containing protease RD21 of Arabidopsis thaliana." PLoS One 7(3): e32422.
Hadlaczky, G., G. Bisztray, T. Praznovszky and D. Dudits (1983). "Mass isolation of plant chromosomes and nuclei." Planta 157(3): 278-285.
Hansen, G. and R. Hilgenfeld (2013). "Architecture and regulation of HtrA-family proteins involved in protein quality control and stress response." Cell Mol Life Sci 70(5): 761-775.
Hartl, F. U., A. Bracher and M. Hayer-Hartl (2011). "Molecular chaperones in protein folding and proteostasis." Nature 475(7356): 324-332.
Hasenbein, S., M. Merdanovic and M. Ehrmann (2007). "Determinants of regulated proteolysis in signal transduction." Genes Dev 21(1): 6-10.
Haussühl, K., B. Andersson and I. Adamska (2001). "A chloroplast DegP2 protease performs the primary cleavage of the photodamaged D1 protein in plant photosystem II." EMBO J 20(4):
713-722.
Hayashi, Y., K. Yamada, T. Shimada, R. Matsushima, N. K. Nishizawa, M. Nishimura and I.
Hara-Nishimura (2001). "A proteinase-storing body that prepares for cell death or stresses in the epidermal cells of Arabidopsis." Plant Cell Physiol 42(9): 894-899.
Hellmann, H. and M. Estelle (2002). "Plant development: regulation by protein degradation."
Science 297(5582): 793-797.
Helm, M., C. Luck, J. Prestele, G. Hierl, P. F. Huesgen, T. Frohlich, G. J. Arnold, I. Adamska, A. Gorg, F. Lottspeich and C. Gietl (2007). "Dual specificities of the glyoxysomal/peroxisomal processing protease Deg15 in higher plants." Proc Natl Acad Sci U S A 104(27): 11501-11506.
Hicks, G. R. and N. V. Raikhel (1995). "Protein import into the nucleus: an integrated view."
Annu Rev Cell Dev Biol 11: 155-188.
Referenzen Higashi, K., R. Takasawa, A. Yoshimori, T. Goh, S. Tanuma and K. Kuchitsu (2005).
"Identification of a novel gene family, paralogs of inhibitor of apoptosis proteins present in plants, fungi, and animals." Apoptosis 10(3): 471-480.
Hoekema, A., P. R. Hirsch, P. J. J. Hooykaas and R. A. Schilperoort (1983). "A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid." Nature 303: 179-180.
Hottiger, T., P. Schmutz and A. Wiemken (1987). "Heat-induced accumulation and futile cycling of trehalose in Saccharomyces cerevisiae." J Bacteriol 169(12): 5518-5522.
Huesgen, P. F. (2007). "Functional genomics of Deg and GCP proteases in photosynthetic organisms." Dissertation Universität Konstanz.
Huesgen, P. F. (2007). "Functional genomics of Deg and GCP proteasesin photosynthetic organisms." Dissertation Universtität Konstanz, Germany.
Huesgen, P. F. and C. M. Overall (2012). "N- and C-terminal degradomics: new approaches to reveal biological roles for plant proteases from substrate identification." Physiol Plant 145(1):
5-17.
Huesgen, P. F., H. Schuhmann and I. Adamska (2005). "The family of Deg proteases in cyanobacteria and chloroplasts of
higher plants." PHYSIOLOGIA PLANTARUM 123: 413-420.
Huesgen, P. F., H. Schuhmann and I. Adamska (2006). "Photodamaged D1 protein is degraded in Arabidopsis mutants lacking the Deg2 protease." FEBS Lett 580(30): 6929-6932.
Huesgen, P. F., H. Schuhmann and I. Adamska (2009). "Deg/HtrA proteases as components of a network for photosystem II quality control in chloroplasts and cyanobacteria." Res Microbiol 160: 726-732.
Hund, C. (2010). "Klonierung von Splicevarianten des DEG7-Gens von Arabidopsis thaliana."
Bachelorarbeit: Universität Konstanz.
Isemer, R., M. Mulisch, A. Schafer, S. Kirchner, H. U. Koop and K. Krupinska (2012).
"Recombinant Whirly1 translocates from transplastomic chloroplasts to the nucleus." FEBS Lett 586(1): 85-88.
Itzhaki, H., L. Naveh, M. Lindahl, M. Cook and Z. Adam (1998). "Identification and characterization of DegP, a serine protease associated with the luminal side of the thylakoid membrane." J Biol Chem 273(12): 7094-7098.
Jarvis, P. and C. Robinson (2004). "Mechanisms of protein import and routing in chloroplasts."
Curr Biol 14(24): R1064-1077.
Joyard, J., M. Ferro, C. Masselon, D. Seigneurin-Berny, D. Salvi, J. Garin and N. Rolland (2010). "Chloroplast proteomics highlights the subcellular compartmentation of lipid metabolism." Prog Lipid Res 49(2): 128-158.
Kalderon, D., B. L. Roberts, W. D. Richardson and A. E. Smith (1984). "A short amino acid sequence able to specify nuclear location." Cell 39(3 Pt 2): 499-509.
Kapri-Pardes, E., L. Naveh and Z. Adam (2007). "The thylakoid lumen protease Deg1 is involved in the repair of photosystem II from photoinhibition in Arabidopsis." Plant Cell 19(3):
1039-1047.
Referenzen
102
Karniely, S. and O. Pines (2005). "Single translation--dual destination: mechanisms of dual protein targeting in eukaryotes." EMBO Rep 6(5): 420-425.
Kaschani, F., M. Shabab, T. Bozkurt, T. Shindo, S. Schornack, C. Gu, M. Ilyas, J. Win, S.
Kamoun and R. A. van der Hoorn (2010). "An effector-targeted protease contributes to defense against Phytophthora infestans and is under diversifying selection in natural hosts." Plant Physiol 154(4): 1794-1804.
Kato, N. and J. Jones (2010). "The split luciferase complementation assay." Methods Mol Biol 655: 359-376.
Kelley, L. A. and M. J. Sternberg (2009). "Protein structure prediction on the Web: a case study using the Phyre server." Nat Protoc 4(3): 363-371.
Kerr, J. F., A. H. Wyllie and A. R. Currie (1972). "Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics." Br J Cancer 26(4): 239-257.
Kim, D. Y. and K. K. Kim (2005). "Structure and function of HtrA family proteins, the key players in protein quality control." J Biochem Mol Biol 38(3): 266-274.
Kim, E., A. Goren and G. Ast (2008). "Alternative splicing: current perspectives." Bioessays 30(1): 38-47.
Kim, Y. E., M. S. Hipp, A. Bracher, M. Hayer-Hartl and F. U. Hartl (2013). "Molecular chaperone functions in protein folding and proteostasis." Annu Rev Biochem 82: 323-355.
Kirwin, P. M., P. D. Elderfield, R. S. Williams and C. Robinson (1988). "Transport of proteins into chloroplasts. Organization, orientation, and lateral distribution of the plastocyanin processing peptidase in the thylakoid network." J Biol Chem 263(34): 18128-18132.
Kley, J., B. Schmidt, B. Boyanov, P. C. Stolt-Bergner, R. Kirk, M. Ehrmann, R. R. Knopf, L.
Naveh, Z. Adam and T. Clausen (2011). "Structural adaptation of the plant protease Deg1 to repair photosystem II during light exposure." Nat Struct Mol Biol 18(6): 728-731.
Kosugi, S., M. Hasebe, M. Tomita and H. Yanagawa (2009). "Systematic identification of cell cycle-dependent yeast nucleocytoplasmic shuttling proteins by prediction of composite motifs."
Proc Natl Acad Sci U S A 106(25): 10171-10176.
Krause, K. and K. Krupinska (2009). "Nuclear regulators with a second home in organelles."
Trends Plant Sci 14(4): 194-199.
Krojer, T., M. Garrido-Franco, R. Huber, M. Ehrmann and T. Clausen (2002). "Crystal structure of DegP (HtrA) reveals a new protease-chaperone machine." Nature 416(6879): 455-459.
Krojer, T., J. Sawa, E. Schafer, H. R. Saibil, M. Ehrmann and T. Clausen (2008). "Structural basis for the regulated protease and chaperone function of DegP." Nature 453(7197): 885-890.
Kurokawa, M. and S. Kornbluth (2009). "Caspases and kinases in a death grip." Cell 138(5):
838-854.
Kushnirov, V. V. (2000). "Rapid and reliable protein extraction from yeast." Yeast 16(9): 857-860.
Kwok, E. Y. and M. R. Hanson (2004). "GFP-labelled Rubisco and aspartate aminotransferase are present in plastid stromules and traffic between plastids." J Exp Bot 55(397): 595-604.
Referenzen Laemmli, U. K. (1970). "Cleavage of structural proteins during the assembly of the head of bacteriophage T4." Nature 227(5259): 680-685.
Lam, E., N. Kato and M. Lawton (2001). "Programmed cell death, mitochondria and the plant hypersensitive response." Nature 411(6839): 848-853.
Lange, A., R. E. Mills, C. J. Lange, M. Stewart, S. E. Devine and A. H. Corbett (2007).
"Classical nuclear localization signals: definition, function, and interaction with importin alpha." J Biol Chem 282(8): 5101-5105.
Lanyon-Hogg, T., S. L. Warriner and A. Baker (2010). "Getting a camel through the eye of a needle: the import of folded proteins by peroxisomes." Biol Cell 102(4): 245-263.
Larkindale, J. and E. Vierling (2008). "Core genome responses involved in acclimation to high temperature." Plant Physiol 146(2): 748-761.
Lazarow, P. B. and Y. Fujiki (1985). "Biogenesis of peroxisomes." Annu Rev Cell Biol 1: 489-530.
Lee, S. J., Y. Matsuura, S. M. Liu and M. Stewart (2005). "Structural basis for nuclear import complex dissociation by RanGTP." Nature 435: 693-696.
Lehninger, A. L. (1987). "Prinzipien der Biochemie." Walter de Gruyter.
Liberek, K., A. Lewandowska and S. Zietkiewicz (2008). "Chaperones in control of protein disaggregation." EMBO J 27(2): 328-335.
Lister, R., O. Chew, M. N. Lee, J. L. Heazlewood, R. Clifton, K. L. Parker, A. H. Millar and J.
Whelan (2004). "A transcriptomic and proteomic characterization of the Arabidopsis mitochondrial protein import apparatus and its response to mitochondrial dysfunction." Plant Physiol 134(2): 777-789.
Lucinski, R., L. Misztal, S. Samardakiewicz and G. Jackowski (2011). "Involvement of Deg5 protease in wounding-related disposal of PsbF apoprotein." Plant Physiol Biochem 49(3): 311-320.
Lucinski, R., L. Misztal, S. Samardakiewicz and G. Jackowski (2011). "The thylakoid protease Deg2 is involved in stress-related degradation of the photosystem II light-harvesting protein Lhcb6 in Arabidopsis thaliana." New Phytol 192(1): 74-86.
Mano, S. and M. Nishimura (2005). "Plant peroxisomes." Vitam Horm 72: 111-154.
Martins, L. M., A. Morrison, K. Klupsch, V. Fedele, N. Moisoi, P. Teismann, A. Abuin, E.
Grau, M. Geppert, G. P. Livi, C. L. Creasy, A. Martin, I. Hargreaves, S. J. Heales, H. Okada, S.
Brandner, J. B. Schulz, T. Mak and J. Downward (2004). "Neuroprotective role of the Reaper-related serine protease HtrA2/Omi revealed by targeted deletion in mice." Mol Cell Biol 24(22):
9848-9862.
Matsuura, Y., A. Lange, M. T. Harreman, A. H. Corbett and M. Stewart (2003). "Structural basis for Nup2p function in cargo release and karyopherin recycling in nuclear import." EMBO J 22(20): 5358-5369.
Matsuura, Y. and M. Stewart (2004). "Structural basis for the assembly of a nuclear export complex." Nature 432(7019): 872-877.
Matsuura, Y. and M. Stewart (2005). "Nup50/Npap60 function in nuclear protein import complex disassembly and importin recycling." EMBO J 24: 3681–3689.
Referenzen
104
Meinke, D. W., J. M. Cherry, C. Dean, S. D. Rounsley and M. Koornneef (1998). "Arabidopsis thaliana: a model plant for genome analysis." Science 282(5389): 662, 679-682.
Merrill, A. H., Jr., G. van Echten, E. Wang and K. Sandhoff (1993). "Fumonisin B1 inhibits sphingosine (sphinganine) N-acyltransferase and de novo sphingolipid biosynthesis in cultured neurons in situ." J Biol Chem 268(36): 27299-27306.
Meyerowitz, E. M. (1987). "Arabidopsis thaliana." Annu Rev Genet 21: 93-111.
Ner-Gaon, H., R. Halachmi, S. Savaldi-Goldstein, E. Rubin, R. Ophir and R. Fluhr (2004).
"Intron retention is a major phenomenon in alternative splicing in Arabidopsis." Plant J 39(6):
877-885.
Neupert, W. (1997). "Protein import into mitochondria." Annu Rev Biochem 66: 863-917.
Nicholson, D. W. and N. A. Thornberry (1997). "Caspases: killer proteases." Trends Biochem Sci 22(8): 299-306.
Nickels, J. T. and J. R. Broach (1996). "A ceramide-activated protein phosphatase mediates ceramide-induced G1 arrest of Saccharomyces cerevisiae." Genes Dev 10(4): 382-394.
Ohta, M., M. Sugita and M. Sugiura (1995). "Three types of nuclear genes encoding chloroplast RNA-binding proteins (cp29, cp31 and cp33) are present in Arabidopsis thaliana: presence of cp31 in chloroplasts and its homologue in nuclei/cytoplasms." Plant Mol Biol 27(3): 529-539.
Onder, O., S. Turkarslan, D. Sun and F. Daldal (2008). "Overproduction or absence of the periplasmic protease DegP severely compromises bacterial growth in the absence of the dithiol:
disulfide oxidoreductase DsbA." Mol Cell Proteomics 7(5): 875-890.
Ostergaard, L. and M. F. Yanofsky (2004). "Establishing gene function by mutagenesis in Arabidopsis thaliana." Plant J 39(5): 682-696.
Padmanabhan, N., L. Fichtner, A. Dickmanns, R. Ficner, J. B. Schulz and G. H. Braus (2009).
"The yeast HtrA orthologue Ynm3 is a protease with chaperone activity that aids survival under heat stress." Mol Biol Cell 20(1): 68-77.
Pallen, M. J. and B. W. Wren (1997). "The HtrA family of serine proteases." Mol Microbiol 26(2): 209-221.
Paschen, S. A. and W. Neupert (2001). "Protein import into mitochondria." IUBMB Life 52(3-5): 101-112.
Peltier, J. B., O. Emanuelsson, D. E. Kalume, J. Ytterberg, G. Friso, A. Rudella, D. A. Liberles, L. Soderberg, P. Roepstorff, G. von Heijne and K. J. van Wijk (2002). "Central functions of the lumenal and peripheral thylakoid proteome of Arabidopsis determined by experimentation and genome-wide prediction." Plant Cell 14(1): 211-236.
Pendle, A. F., G. P. Clark, R. Boon, D. Lewandowska, Y. W. Lam, J. Andersen, M. Mann, A. I.
Lamond, J. W. Brown and P. J. Shaw (2005). "Proteomic analysis of the Arabidopsis nucleolus suggests novel nucleolar functions." Mol Biol Cell 16(1): 260-269.
Ponting, C. P. (1997). "Evidence for PDZ domains in bacteria, yeast, and plants." Protein Sci 6(2): 464-468.
Referenzen Pontvianne, F., I. Matia, J. Douet, S. Tourmente, F. J. Medina, M. Echeverria and J. Saez-Vasquez (2007). "Characterization of AtNUC-L1 reveals a central role of nucleolin in nucleolus organization and silencing of AtNUC-L2 gene in Arabidopsis." Mol Biol Cell 18(2): 369-379.
Powers, E. T. and W. E. Balch (2013). "Diversity in the origins of proteostasis networks--a driver for protein function in evolution." Nat Rev Mol Cell Biol 14(4): 237-248.
Rawlings, N. D. and A. J. Barrett (1993). "Evolutionary families of peptidases." Biochem J 290 ( Pt 1): 205-218.
Rawlings, N. D., F. R. Morton and A. J. Barrett (2006). "MEROPS: the peptidase database."
Nucleic Acids Res 34(Database issue): D270-272.
Rawlings, N. D., F. R. Morton, C. Y. Kok, J. Kong and A. J. Barrett (2008). "MEROPS: the peptidase database." Nucleic Acids Res 36(Database issue): D320-325.
Reape, T. J. and P. F. McCabe (2010). "Apoptotic-like regulation of programmed cell death in plants." Apoptosis 15(3): 249-256.
Reape, T. J., E. M. Molony and P. F. McCabe (2008). "Programmed cell death in plants:
distinguishing between different modes." J Exp Bot 59(3): 435-444.
Rhee, S. Y., W. Beavis, T. Z. Berardini, G. Chen, D. Dixon, A. Doyle, M. Garcia-Hernandez, E.
Huala, G. Lander, M. Montoya, N. Miller, L. A. Mueller, S. Mundodi, L. Reiser, J. Tacklind, D.
C. Weems, Y. Wu, I. Xu, D. Yoo, J. Yoon and P. Zhang (2003). "The Arabidopsis Information Resource (TAIR): a model organism database providing a centralized, curated gateway to Arabidopsis biology, research materials and community." Nucleic Acids Res 31(1): 224-228.
Rosso, M. G., Y. Li, N. Strizhov, B. Reiss, K. Dekker and B. Weisshaar (2003). "An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics." Plant Mol Biol 53(1-2): 247-259.
Saez-Vasquez, J., D. Caparros-Ruiz, F. Barneche and M. Echeverria (2004). "A plant snoRNP complex containing snoRNAs, fibrillarin, and nucleolin-like proteins is competent for both rRNA gene binding and pre-rRNA processing in vitro." Mol Cell Biol 24(16): 7284-7297.
Sambrook, J., E. F. Fritsch and T. Maniatis (1989). "Molecular cloning: a laboratory manual."
Cold Spring Harbor Laboratory Press.
Schleiff, E. and T. Becker (2011). "Common ground for protein translocation: access control for mitochondria and chloroplasts." Nat Rev Mol Cell Biol 12(1): 48-59.
Schmid, M., T. S. Davison, S. R. Henz, U. J. Pape, M. Demar, M. Vingron, B. Scholkopf, D.
Weigel and J. U. Lohmann (2005). "A gene expression map of Arabidopsis thaliana development." Nat Genet 37(5): 501-506.
Schubert, M., U. A. Petersson, B. J. Haas, C. Funk, W. P. Schroder and T. Kieselbach (2002).
"Proteome map of the chloroplast lumen of Arabidopsis thaliana." J Biol Chem 277(10): 8354-8365.
Schuhmann, H. (2008). "Deg Proteases in Arabidopsis thaliana " Dissertation Universtität Konstanz.
Schuhmann, H. and I. Adamska (2012). "Deg proteases and their role in protein quality control and processing in different subcellular compartments of the plant cell." Physiol Plant 145(1):
224-234.
Referenzen
106
Schuhmann, H., P. F. Huesgen, C. Gietl and I. Adamska (2008). "The DEG15 serine protease cleaves peroxisomal targeting signal 2-containing proteins in Arabidopsis." Plant Physiol 148(4): 1847-1856.
Schuhmann, H., U. Mogg and I. Adamska (2011). "A new principle of oligomerization of plant DEG7 protease based on interactions of degenerated protease domains." Biochem J 435(1): 167-174.
Seibel, N. M., J. Eljouni, M. M. Nalaskowski and W. Hampe (2007). "Nuclear localization of enhanced green fluorescent protein homomultimers." Anal Biochem 368(1): 95-99.
Seki, M., M. Narusaka, A. Kamiya, J. Ishida, M. Satou, T. Sakurai, M. Nakajima, A. Enju, K.
Akiyama, Y. Oono, M. Muramatsu, Y. Hayashizaki, J. Kawai, P. Carninci, M. Itoh, Y. Ishii, T.
Arakawa, K. Shibata, A. Shinagawa and K. Shinozaki (2002). "Functional annotation of a full-length Arabidopsis cDNA collection." Science 296(5565): 141-145.
Sessions, A., E. Burke, G. Presting, G. Aux, J. McElver, D. Patton, B. Dietrich, P. Ho, J.
Bacwaden, C. Ko, J. D. Clarke, D. Cotton, D. Bullis, J. Snell, T. Miguel, D. Hutchison, B.
Kimmerly, T. Mitzel, F. Katagiri, J. Glazebrook, M. Law and S. A. Goff (2002). "A high-throughput Arabidopsis reverse genetics system." Plant Cell 14(12): 2985-2994.
Shaw, P. J. and J. W. Brown (2004). "Plant nuclear bodies." Curr Opin Plant Biol 7(6): 614-620.
Shi, L. X. and S. M. Theg (2013). "The chloroplast protein import system: from algae to trees."
Biochim Biophys Acta 1833(2): 314-331.
Shieh, M. W., S. R. Wessler and N. V. Raikhel (1993). "Nuclear targeting of the maize R protein requires two nuclear localization sequences." Plant Physiol 101(2): 353-361.
Shindo, T., J. C. Misas-Villamil, A. C. Horger, J. Song and R. A. van der Hoorn (2012). "A role in immunity for Arabidopsis cysteine protease RD21, the ortholog of the tomato immune protease C14." PLoS One 7(1): e29317.
Skorko-Glonek, J., D. Zurawa, E. Kuczwara, M. Wozniak, Z. Wypych and B. Lipinska (1999).
"The Escherichia coli heat shock protease HtrA participates in defense against oxidative stress."
Mol Gen Genet 262(2): 342-350.
Small, I., H. Wintz, K. Akashi and H. Mireau (1998). "Two birds with one stone: genes that encode products targeted to two or more compartments." Plant Mol Biol 38(1-2): 265-277.
Smalle, J. and R. D. Vierstra (2004). "The ubiquitin 26S proteasome proteolytic pathway." Annu Rev Plant Biol 55: 555-590.
Soto, C., L. Estrada and J. Castilla (2006). "Amyloids, prions and the inherent infectious nature of misfolded protein aggregates." Trends Biochem Sci 31(3): 150-155.
Spiess, C., A. Beil and M. Ehrmann (1999). "A temperature-dependent switch from chaperone to protease in a widely conserved heat shock protein." Cell 97(3): 339-347.
Srinivasula, S. M., S. Gupta, P. Datta, Z. Zhang, R. Hegde, N. Cheong, T. Fernandes-Alnemri and E. S. Alnemri (2003). "Inhibitor of apoptosis proteins are substrates for the mitochondrial serine protease Omi/HtrA2." J Biol Chem 278(34): 31469-31472.
Stone, J. M., J. E. Heard, T. Asai and F. M. Ausubel (2000). "Simulation of fungal-mediated cell death by fumonisin B1 and selection of fumonisin B1-resistant (fbr) Arabidopsis mutants."
Plant Cell 12(10): 1811-1822.
Referenzen
Stroher, E. and K. J. Dietz (2008). "The dynamic thiol-disulphide redox proteome of the
Stroher, E. and K. J. Dietz (2008). "The dynamic thiol-disulphide redox proteome of the