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(1)Additional file 1: Oligonucleotides used for recombinant expression of S

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Additional file 1: Oligonucleotides used for recombinant expression of S. suis antigens in E. coli.

Name Sequence (5’-3’)

0934(-21aa)-F AGGCGGTACCGGTAACCGTGCTTCAAAAAGCA 0934-R AGGCAAGCTTTTAAGGTTTTTCAGGAACTTCTAC 1869(-20aa)-F AGGCGAGCTCCGTCCATCGCAAACCACAGAA 1869-R AGGCGTCGACCTATTTCAGATATTTAACCATCAGT 757Pro-F AGAGGATCCGATGAATTGACAAGCCTTGTAG 757Pro-R GGGGTCGACTTAGACTTCTTCTTTATGACG 1950(-30aa)-F GGCGGTACCGAAACCTATACTGTAAAGTCTGG 1950-R GGCAAGCTTTTAGTACCAACCATTTGCCAAC 1664(-31aa)-F GGCGAGCTCGCAAAACAGGAATTGTCATTCC 1664-R GGCGTCGACTTATTCTGCCACTACACCCTTA 0187-F GGCGAGCTCATGCGCTTTAATCAATTTTCTTTCA 0187(-stop)-R GGCAAGCTTGTGCGGTAAAATTAACTGTGATT

pQE80L-strep-F AGCGCGTGGAGCCATCCGCAGTTTGAAAAATAAAATTAGCTGAGCTTGGACTCCT pQE80L-HindIII-R AAGCTTGGCTGCAGGTCGAC

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