sh-NC si-scramble si-Serpine1
sh-circ-GALNT16 si-scramble si-Serpine1
DLD-1LoVo
A
vector vector Serpine1
circ-GALNT16 vector Serpine1
RKOHCT 116
D
DLD-1 LoVo
C
0.0 0.5 1.0 1.5
RelativeexpressionofSerpine1 si-scamble si-Serpine1
** **
RKO HCT 116
0 2 4 6
RelativeexpressionofSerpine1 vector Serpine1
**
**
si-Serpine1 Vector Serpine1
si-scramble
DLD-1 LoVo RKO HCT-116
GAPDH Serpine1
si-Serpine1
si-scramble Vector Serpine1
36kDa 50kDa
B
E
RKO HCT 116
0 50 100 150 200 250
Cellcounts
vector vector + Serpine1 circ-GALNT16 + vector circ-GALNT16 + Serpine1
*
** ** **
DLD-1 LoVo
0 100 200 300
Cellcounts
sh-NC + si-scramble sh-NC + si-Serpine1 sh-circ-GALNT16 + si-scramble sh-circ-GALNT16 + si-Serpine1
**
**
**
**
sh-NC si-scramble si-Serpine1
sh-circ-GALNT16 si-scramble si-Serpine1
DLD-1LoVo
100μm
vector vector Serpine1
circ-GALNT16 vector Serpine1
RKOHCT 116
F
RKO HCT 116
0 20 40 60 *
** **
* vector vector + Serpine1 circ-GALNT16 + vector circ-GALNT16 + Serpine1
DLD-1 LoVo
0 20 40 60
Thepercentageof EDUpositivecells(%)
**
**
**
**
sh-NC + si-scramble sh-NC + si-Serpine1 sh-circ-GALNT16 + si-scramble sh-circ-GALNT16 + si-Serpine1
G
0 1 2 3 4 5 0.0
0.5 1.0 1.5 2.0 2.5
Days
CCK8(OD450nm)
sh-NC + si-scramble sh-NC + si-Serpine1 sh-circ +si-scramble
DLD-1
sh-circ + si-Serpine1
**
**
0 1 2 3 4 5 0.0
0.5 1.0 1.5 2.0
Days
CCK8(OD450nm)
sh-NC+si-scramble sh -NC+si-Serpine1 sh-circ+si-scramble
LoVo
sh-circ+si-Serpine1
**
**
0 1 2 3 4 5
0.0 0.5 1.0 1.5 2.0
Days
CCK8(OD450nm)
vector HCT-116
circ+vector vector+Serpine1 circ+Serpine1
**
**
vector circ+vector vector+Serpine1 circ+Serpine1
0 1 2 3 4 5
0.0 0.5 1.0 1.5 2.0 2.5
Days
CCK8(OD450nm)
RKO
****
Thepercentageof EDUpositivecells(%)
100μm
