Masse / Ladung
4.3 Anregungen für weitere Untersuchungen
Die 3-Hydroxybenzylsuccinat-Synthase und Benzylsuccinat-Synthase konnten in D.
cetonicum mit vergleichsweise hoher spezifischen Aktivität gemessen werden. Dieser Umstand könnte Studien zum Mechanismus dieser Reaktionen erleichtern, zumal in T.
aromatica aufgrund der labilen Aktivität der Benzylsuccinat-Synthase mechanistische Studien erschwert sind. Das vermutete Vorhandensein einer inaktiven, sauerstoff-unempfindlichen Form der 3-Hydroxybenzylsuccinat-Synthase und evtl. auch Benzylsuccinat-Synthase in D.
cetonicum könnte die Möglichkeit zu Studien zum Reaktionsmechanismus zusätzlich erleichtern. Falls die Reaktion tatsächlich über ein Glycyl- bzw. Cysteinyl-Radikal katalysiert
wird, so könnten sich Untersuchungen zur Generierung eines solchen Radikals ergeben. Somit wäre an eine Reinigung der 3-Hydroxybenzylsuccinat- oder Benzylsuccinat-Synthase aus D.
cetonicum zu denken.
Für den anaeroben p-Kresolabbau in D. cetonicum deutet sich ein ähnlicher Verlauf an wie für den m-Kresolabbau beschrieben. Eine Synthese von 4-Hydroxybenzylsuccinat sollte als nächster Schritt erfolgen. Wenn sich die Annahme der in vitro Bildung von 4-Hydroxybenzylsuccinat bestätigen läßt, so können ähnliche Untersuchungen durchgeführt werden, die zur Aufklärung des m-Kresolabbaus ausgeführt wurden.
Für 3-Hydroxybenzoat kann angenommen werden, daß unter denitrifizierenden Bedingungen ein Abbau über Hydroxyhydrochinon erfolgt. Zur weiteren Absicherung dieser Hypothese könnte versucht werden, Intermediate der in vitro 3-Hydroxybenzoat-Oxidation über GC/MS nachzuweisen. Entsprechende Experimente könnten in H218O durchgeführt werden, um die Herkunft des Sauerstoffs neu eingeführter Hydroxylgruppen eindeutig zu belegen. Zusätzlich sollte untersucht werden, inwieweit die entsprechenden oxidierenden Enzymaktivitäten konstitutiv sind oder beim Abbau von 3-Hydroxybenzoat induziert werden.
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