10 15 25 40 35
KDa KDa
M = ThermoFischer PageRuler™ Pre-stained Protein Ladder - 10 to 180 kDa (order # 26616) WB = non-transgenic cells
WCL = HA-GiCHMP7 whole cell lysate
F-S = HA-GiCHMP7 fractionated sample enriched for soluble components F-M = HA-GiCHMP7 fractionated sample enriched for membrane components
Western Blot Coomassie Gel
M WB M
WB HA-GiCHMP7WCL
HA-GiCHMP7WCL HA-GiCHMP7F-S
HA-GiCHMP7F-S HA-GiCHMP7F-M
HA-GiCHMP7F-M
55 40 70
35 25
15 10 180130 100
A B
Supplementary Figure 9