1 S3 Table. Primers used in this study.
1
Primer/Vector Sequence* Amplified DNA fragment
CspAB31pfp/pQE30Xa accGGATCCgcaccttttagc cspAB31p CspAB31prp/pQE30Xa tttcGAATTCttagtaaaaggcaggtttta
aag
CspAZQ1pfp/pQE30Xa cgGGATCCgttgataaaattgatcca cspAZQ1p CspAZQ1prp/pQE30Xa cgGGATCCttaatagtcaagatttaa
CspAB31L246Dpfp/pQE 30Xa
accGGATCCgcaccttttagc cspAB31L246Dp
CspAB31L246Dprp/pQE 30Xa
tcGTCGACttagtaaaaggcaggtttatca gtatc
pcspAB31fp/pBSV2G cgGCATGCttacagctacaagaaaag pcspAB31
pcspAB31rp/pBSV2G cgGTCGACaatactctccttataaat
CspAB31fp/pBSV2G cgGTCGACttgaaaaaagccaaacta cspAB31
CspAB31rp/pBSV2G cgGGATCCttagtaaaaggcaggttt
CspAPKofp/pBSV2G cgGTCGACttgacaaaaaccaacctg cspAPKo
CspAPKorp/pBSV2G cgGGATCCctaattaatgggattaaa
CspAZQ1fp/pBSV2G cgGTCGACttgaaaaaaactaaactt cspAZQ1
CspAZQ1rp/pBSV2G cgGGATCCctaattaatgggattaaa CspAB31L246Dfp/pBS
V2G
cgGTCGACttgaaaaaagccaaacta cspAB31L246D
CspAB31L246Drp/pBS V2G
tcGGATCCttagtaaaaggcaggtttatca gtatc
BBCspAfp cttaaacgaaactcttaaagtttacaatca BBcspAB31
BBCspArp ttagtaaaaggcaggttttaaagtatcaaa
BBFlaBfp gcagctaatgttgcaaatcttttc BBflab BBFlaBrp gcaggtgctggctgttga
BBRecAfp gtggatctattgtattagatgaggctctcg BBrecA BBRecArp gccaaagttctgcaacattaacacctaaag
2 ColE1fp ctacatacctcgctctgctaatc BBcolE1
ColE1rp cgaaacccgacaggactataaa
mNidfp ccagccacagaatcccatcc mNidogen
mNidrp ggacatactctgctgccatc
Kanfp atgagccatattcaacgggaa Kanamycin
Kanrp ttagaaaaactcatcgagcat
Genfp atgttacgcagcagcaac Gentamycin
Genrp ttaggtggcggtacttgg
Strfp caggatgacgcctaacaa Streptomycin
Strrp ccaccttcaacagatcgc
* Restriction sites used are shown in capital letters.
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