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Supplementary table 1. Oligonucleotides used in this study

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Supplementary table 1. Oligonucleotides used in this study

Oligonucleotide Sequence (5’-3’) Use in this work

CbiA_K153A FWD CACAACCACTTTAAGTCAAGCAAAGGAACGAG CACTAGAATA

Generation of CbiA harboring single amino acid substitutions K153A CbiA_K153A REV GACGCTTTATATATTCTAGTGCTCGTTCCTTTG

CTTGACTTAAAG

Generation of CbiA harboring single amino acid substitutions K153A CbiA_K154A FWD CACAACCACTTTAAGTCAAAAAGCGGAACGAG

CACTAGAATA

Generation of CbiA harboring single amino acid substitutions K154A CbiA_K154A REV GACGCTTTATATATTCTAGTGCTCGTTCCGCTT

TTTGACTTAAAG

Generation of CbiA harboring single amino acid substitutions K154A CbiA_K162A FWD CGAGCACTAGAATATATAGCGCGTCAATTATA

CATTG

Generation of CbiA harboring amino acid substitutions K162A and K154A- K162A

CbiA_K162A REV GGAAATAAAATTCAATGTATAATTGACGCGCT ATATATTC

Generation of CbiA harboring amino acid substitutions K162A and K154A- K162A

CbiA_K188A FWD CTTCCAAAGAACAATAGCACTTTTAGAAACTC AAAG

Generation of CbiA harboring amino acid substitutions K188A and K154A- K162A-K188A

CbiA_K188A REV CAAAAATTAACTTTGAGTTTCTAAAAGTGCTAT TGTTC

Generation of CbiA harboring amino acid substitutions K188A and K154A- K162A-K188A

CbiA_K153A/

K154A FWD

CACAACCACTTTAAGTCAAGCAGCGGAACGAG CACTAGAATA

Generation of CbiA harboring amino acid substitutions K153A- K154A- K162A-K188A

CbiA_K153A/

K154A REV

GCGCTATATATTCTAGTGCTCGTTCCGCTGCTT GACTTAAAGTG

Generation of CbiA harboring amino acid substitutions K153A- K154A- K162A-K188A

CbiA_R145A FWD CTTTTCATCATCAAATTTTGCAGACACAACCAC TTTAAG

Generation of CbiA harboring single amino acid substitutions R145A CbiA_R145A REV CCTTTTTTTGACTTAAAGTGGTTGTGTCTGCAA

AATTTGATG

Generation of CbiA harboring single amino acid substitutions R145A CbiA_R156A FWD CTTTAAGTCAAAAAAAGGAAGCAGCACTAGAA

TATATAAAG

Generation of CbiA harboring single amino acid substitutions R156A CbiA_R156A REV GACGCTTTATATATTCTAGTGCTGCTTCCTTTTT

TTGAC

Generation of CbiA harboring single amino acid substitutions R156A CbiA_R163A FWD CACTAGAATATATAAAGGCACAATTATACATT

GAA

Generation of CbiA harboring single amino acid substitutions R163A CbiA_R163A REV GGAAATAAAATTCAATGTATAATTGTGCCTTT

ATATATTC

Generation of CbiA harboring single amino acid substitutions R163A CbiA_R185A FWD CAGAATTTTTCTTCCAAGCAACAATAAAACTTT

TAG

Generation of CbiA harboring single amino acid substitutions R185A CbiA_R185A REV GAGTTTCTAAAAGTTTTATTGTTGCTTGGAAGA

AAAAT

Generation of CbiA harboring single amino acid substitutions R185A

flaB Fw_PBi TTCAATCAGGTAACGGTACA qRT-PCR

flaB Rv_PBi GAAGCTTGAGATCCTGAAAG qRT-PCR

pQE-FP-30 TTGCTTTGTGAGCGGATAAC Sequencing

pQE-RP CTGAGGTCATTACTGGATCTATC Sequencing

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