Supplementary Table 1: Primer sequences used in the work.
Gene Primer sequence
(5’-3’)
10μM primers, volume (μL), F+R
Annealing Temperature
(°C) NANOG F: TGAACCTCAGCTACAAACAGGTGR: AACTGCATGCAGGACTGCAGAG 0.5 60 OCT3/4 F: CTTGCTGCAGAAGTGGGTGGAGGAAR: CTGCAGTGTGGGTTTCGGGCA 0.4 64 SOX2 F: AGAACCCCAAGATGCACAACR: CGGGGCCGGTATTTATAATC 0.5 60 PAX6 F: AACGATAACATACCAAGCGTGTR: GGTCTGCCCGTTCAACATC 0.5 62 METTL3 F: CAAGCTGCACTTCAGACGAAR: GCTTGGCGTGTGGTCTTT 0.5 60 FTO F: GAAAATCTGCTGGACAGCTCAR: CGAGAGAGTCATCCTCACTT 0.5 60
YTHDF3 Gene expression assay N/A 60
VIRMA Gene expression assay N/A 60
ALKBH5 Gene expression assay N/A 60
WTAP F: TTCCCAAGAAGGTTCGATTGR: TGCAGACTCCTGCTGTTGTT 0.5 60 METTL14 R: TCTTCTTCATATGGCAAATTTTCTTF: AGAAACTTGCAGGGCTTCCT 0.5 60 RAD9 R: CCAAGGGTTCCAGGTAGAGCF: GTGAAGGTGCTCGGCAAGG 0.5 60 GADD45
A
F: AGCAGAAGACCGAAAGGATGG
0.3 60
R: TGACTCAGGGCTTTGCTGAG
GADD45B F: CTGGTCACGAACCCTCACACR: CTTTCTTCGCAGTAGCTGGC 0.5 60
METTL4 Gene expression assay N/A 60
18SrRNA Gene expression assay N/A 60
GUSB R: TGGACCAGGTTGCTGATGTCF: ATGCCATCGTGTGGGTGAAT 0.5 60
