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Table S11. Primers used for mRNA analysis of

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Table S11. Primers used for mRNA analysis of SHANK2 isoforms.

Exon Amplicon name

Amplicon

size (bp) Forward primer (5'-3') Reverse primer (5'-3') Annealing E6-E10 SHANK2E 445 GATGACCGCCCTACACAAAG CAGCTCAAAGTTGCCTGCTA 58°C E6-E7 SHANK2E* 81 AGTTGCCCTGAAGACCCTTT GTGTGATACAGCGGGGTGAG 60°C E1b-

E11 ProSAP1A 305 GAAGTCTTTGTTAAATGCCTTCAC CCAGCCTGTTGAGCGATG 57°C E1c-

E16 ProSAP1 252 GTGATGATGACGGGCTACAA GAAGTCCCCGGTCCTTAGTC 55°C E15-

E17

All isoforms or PDZ

domain 333 TTGAGGAGAAGACGGTGGTC TTTCTTCCTGGCGGTGTCG 58°C E18-

E21 Splicing E19 & E20 154 ACCGACCACAGCCCTCAC TTCCACAGCCATGTTCTCAG 55°C E21-22 AF141901 456 CTGAGAACATGGCTGTGGAA TGGGCTTCAAGATGACAGAA 55°C E21-24 Splicing E23 404 CTGAGAACATGGCTGTGGAA CGTCCCGTAGACTCTTGGAG 55°C

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