Table S1. PCR primers, target loci, and thermal cycles used in this study.
Primer Locu s
PCR cycle References
LCO-1490 COI 3 min 95°, 35 cycles of (1 min 95°, 1 min 40°, 1.5 min 72°), 7 min 72° C
Folmer et al. 1994 16Sar-b 16S 3 min 95°, 35 cycles of (30 sec 94°, 30 sec
49°, 1 min 72°), 5 min 72° C Palumbi 1994 Brandner et al. 2017 16Sbr-H 16S 3 min 95°, 35 cycles of (30 sec 94°, 30 sec
49°, 1 min 72°), 5 min 72° C Palumbi 1994 Brandner et al. 2017 ECOIC COI 3 min 95°, 35 cycles of (30 sec 94°, 30 sec
49°, 1 min 72°), 5 min 72° C
Hrincevich and Foltz 1996 Foltz et al. 2007
18S-R 18S 3 min 95°, 35 cycles of (30 sec 94°, 30 sec 49°, 1 min 72°), 5 min 72° C
Hrincevich and Foltz 1996 Foltz et al. 2007
18S-1F 18S 3 min 95°, 35 cycles of (30 sec 94°, 30 sec 49°, 1 min 72°), 5 min 72° C
Fuchs et al. 2009
Table S2. Number of specimens sequenced per morphotype.
Larval morphotype Primer used No. PCRs No. sequences No. matches
Golfingia elongata LCO-1490 2 1 1
16Sar-b 2 1 0
16Sbr-H 2 2 0
Embryo LCO-1490 2 1 0
16Sar-b 1 1 1
16Sbr-H 1 0 0
Nipponemertes pulchra LCO-1490 1 1 0
16Sar-b 1 0 0
16Sbr-H 2 2 0
Bivalve veliger 16Sar-b 2 1 0
16Sbr-H 5 4 0
Nudibranch veliger LCO-1490 1 1 0
16Sbr-H 2 2 0
Alcyonidium sp. LCO-1490 3 1 1
18S-1F 3 3 0
Cerianthus lloydi LCO-1490 1 1 0
ECOIC 1 1 0
18S-R 1 1 0
Asteroid LCO-1490 1 1 0
ECOIC 2 2 0
18S-R 2 2 0
Parenchymella LCO-1490 1 1 0
16Sbr-H 1 1 0
Polychaete1 LCO-1490 3 0 0
Unknown LCO-1490 1 0 0
Total 44 31 3
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