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S. 1. Primers used in the study

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S. 1. Primers used in the study

Primer name Sequence 5’  3’ Application

mtlD_up_fwd GTTAGCGGCCGCAACAGCTTGAGGATATGCAGAG

Deletion of mtlD in ATCC 19606 mtlD_up_rev GCCGGGATCCCTTTGGACATTAAGTCGAAC

mtlD_down_fwd CTGCGGATCCCATCCATATCGAAGATAGCTC mtlD_down_rev GTTGCTGCAGGTACTACGCTTCTACCAAACC mtlD_ctr_fwd GCTGTACCGCAGTCAATCAC

mtlD_ctr_rev GTGTCGTTCTGGTGCCAGAAG

mtlD_fwd GCCGCATATGGTGCTTATTTTTCATGGAAAACC

MtlD purification mtlD_rev ATGGCGCGGCCGCAACATATTGAGTTGATGTAGTTTTG

mtlD_up_gusA_fwd GGCGTCTAGAGCCGAAATTTATGAAGGGAAGC Reporter gene assay mtlD_up_gusA_rev CGCGCCATGGGTTTTTACCTCTTTTTTGATCATG

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