Development of analytical methods for the simultaneous determination of the Fusarium toxins zearalenone, deoxynivalenol and their metabolites in physiological
samples of dairy cows and verification through a dose-response feeding study
Von der Fakultät für Lebenswissenschaften der Technischen Universität Carolo-Wilhelmina
zu Braunschweig zur Erlangung des Grades einer Doktorin der Naturwissenschaften
(Dr. rer. nat.) genehmigte D i s s e r t a t i o n
Kumulative Arbeit
von Janine Winkler
aus Chemnitz
Table of Contents
Danksagung... I List of Tables... II List of Figures... Ill Abbreviations... IV
Summary...1
P a r t i ... 3
1.1 Introduction...4
1.1.1 Fusarium mycotoxins... 5
1.1.2 Characteristics and mode of action of ZEN and D O N ...6
1.1.3 Metabolism and kinetics of ZEN and DON in ruminants... 8
1.1.4 Effects of ZEN and DON on health and performance...11
11.5 Analytical M ethods...】4
1.2 Scope of the thesis...18
P a r t2 ...19
Paper I: Residues of zearalenone (ZEN), deoxynivalenol (DON) and their metabolites in plasma of dairy cows fed Fusarium contaminated maize and their relationships to performance parameters... 20
Paper II: Simultaneous determination of zearalenone, deoxynivalenol and their metabolites in bovine urine as biomarkers of exposure 47 Paper III: Diagnostic opportunities for evaluation of the exposure of dairy cows to the mycotoxins deoxynivalenol (DON) and zearalenone (ZEN): Reliability 0Î blood plasma，bile and follicular fluid as indicators... 72
Paper IV: Development of a multi-toxin method for investigating the carryover of zearalenone, deoxynivalenol and their metabolites into milk of dairy cows 90
P a rt3 ...110
General Discussion 111
