A6 Alignment der putativen Kondensationsdomänen C 1 und C 2 der Esyn aus F. scirpi
7.9 A9 Karten der, für die Klonierung des esyn1 Gens in Streptomyces spp., benutzten Vektoren
pSP72
SphI
PstI KpnI
BamHI XhoI
ClaI EcoRV
SstI BamHI/
BglI BglI EcoRV ClaI EcoRI SstI KpnI SmaI
ps p7
2
pI J7 02
pspIJ001 7,9kb
2, 4k
b
melP
tsr
7.10 A10 Listing des Programms „Peptidanalyse“ Dr. F. Mallwitz
PRINT AT(17,4);"Programm zu Berechnung und Vergleich von Aminosäuresequenzen ;-)"
REM Sequenz in Stringfeld SE$ (A) stapeln REM Sequenz besteht aus 3119 AS in 13 Blöcken DIM A$(14)
OPEN "i",#2,"a:\S2.txt"
FOR I = 1 TO 13
REM Sequenz ins Feld einsortieren
DIM SE$ (z,2)
REM Liefert das Feld die Zahl an AS Z und das Feld SE$(z,1) gefüllt mit den As N-Terminal beginnend
REM ***********************einlesen beendet************************
RETURN
INPUT "Anzahl der Paare von Aminosäuren";AS PRINT
PRINT " Falls du dich vertippt hast , gehts mit >RETURN< zurück zur Eingabe "
REM Umsortieren und zusammenfassen der Sequenzen
PRINT "Das sind alle möglichen Sequenzpaare "
REM Berechnen aller Sequenzpaare FOR I = 1 TO max
REM aussortieren der doppelten Sequenzpaare (Spiegelproblem)
FOR I = 1 TO max
DIM AS2(max,50)
PRINT " Ergebnis der Sequenzsuche (Häufigkeit und Position der ersten 10 Treffer) "
PRINT " Eingabe der AS Sequenzen wonach das verwendete Enzym schneidet"
PRINT " ( C- Terminal )"
PRINT " Falls du dich vertippt hast, geht’s mit >RETURN< zurück zur Eingabe "
PRINT INPUT a$
EXIT IF a$ = "Y" OR a$ = "y"
LOOP
FOR I = 1 TO N1
flag1 = 1
PRINT " Ergebnis der Schnittstellensuche (die ersten 12 Schnittstellen werden angezeigt)"
INPUT "Eingabe der AS von der aus die Darstellung begonnen wird";nr2 CLS
E = nr2 + 400 - 1
IF E > ASmax THEN E = ASmax
PRINT AT(2,2);" Darstellung der AS von Nr. ";nr2;" (oben links) bis Nr. ";E;" (unter rechts)"
flag1 = 0
PRINT " Wenn Du davon genug hast versuch ´s mit Y und <RETURN>"
PRINT " Wenn´ s Dir noch gefällt nur <RETURN>"
INPUT a$
EXIT IF a$ = "Y" OR a$ = "y"
LOOP RETURN
PROCEDURE tschau CLS
FOR I = 1 TO 10 PRINT NEXT I
PRINT " Und tschüß ;-) "
PAUSE 50 CLS RETURN
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