KNOCK ‐ OUT IN CELL LINES VIA TALEN TECHNOLOGY
II.12. M ATERIAL , EQUIPMENT , AND SOFTWARE
II.12.9. C OLUMNS , RESINS , AND SPE CARTRIDGES
columns, resins, and SPE cartridges producer / supplier
Bio‐Scale Mini Bio‐Gel P‐6 Desalting cartridge (10 ml) Bio‐Rad Laboratories GmbH Bio‐Scale Mini Profinity IMAC cartridge (1 ml) Bio‐Rad Laboratories GmbH Econo‐Pac Chromatography column Bio‐Rad Laboratories GmbH
HisTrap HP (1 ml) GE Healthcare Life Science GmbH
Kinetex 2.6 μm C18 100 Å 50 mm x 2.1 mm Phenomenex®, Inc.
Kinetex 2.6 μm C18 100 Å 50 mm x 4.6 mm Phenomenex®, Inc.
Luna 5 μm C18(2) 100 Å 125 mm x 4 mm Phenomenex®, Inc.
MACS® Separation Columns Miltenyi Biotec GmbH
Ni Sepharose High Performance resin GE Healthcare Life Science GmbH SecurityGuard Standard C18 4 mm x 2 mm Phenomenex®, Inc.
Synergy 4 μm Fusion‐RP 80 Å 150 mm x 4.6 mm Phenomenex®, Inc.
Strata C18‐E 55 μm reversed phase (100mg/ml) tube Phenomenex®, Inc.
Strata‐X 33 μm polymeric reversed phase (30mg/ml) tube Phenomenex®, Inc.
II.12.10. E
QUIPMENTCH R O M A T O G R A P H Y S Y S T E M S
FPLC system producer
ProfiniaTM Affinity Chromatography Protein Purification System
Bio‐Rad Laboratories GmbH
HPLC‐UV autosampler, pump, degasser, and detector producer
Series 200 Autosampler Perkin Elmer®, Inc.
Series 200 Micro Pump Perkin Elmer®, Inc.
Series 200 Mixer Perkin Elmer®, Inc.
Series 200 UV/Vis Detector Perkin Elmer®, Inc.
Series 200 Vacuum Degasser Perkin Elmer®, Inc.
radioactive HPLC autosampler, pump, degasser, and detector producer
LB 506 D Radioactivity Monitor Berthold GmbH & Co. KG
LC‐502E Autosampler Beckman Coulter, Inc.
MCP‐Z Standard gear pump Ismatec®
System Gold® 125 Solvent Module Beckman Coulter, Inc.
System Gold® 166 Detector Beckman Coulter, Inc.
LC‐MS/MS autosampler, pump, degasser, and detector producer
1260 Infinity degasser Agilent Technologies, Inc.
1260 Infinity binary pump model G1312B Agilent Technologies, Inc.
1260 TCC column oven model G1316A Agilent Technologies, Inc.
HTC‐xt autosampler CTC Analytics AG (PALTM)
QTrap® 5500 mass spectrometer AB SciexTMLtd.
DE T E CT I O N S Y S T E M S
detection system producer / supplier
ABI 3730 DNA Analyzer Applied Biosystems®
AxioImager Z1/ApoTome confocal microscope Cary®50 UV‐Visible spectrophotometer Varian, Inc.
Fusion FX7 Vilber Lourmat GmbH
GloMax®‐Multi Detection System + Dual Injector System
Promega GmbH Promega GmbH
NanoDropTM ND‐1000 PeqLab Biotechnologie GmbH
Odyssey® Infrared Imaging System LI‐COR®Biosciences GmbH
RF‐5000 fluorimeter Shimadzu Scientific Instruments, Inc.
Safire II plate reader Tecan Group Ltd.
Synergy 2 Multi‐Mode Microplate reader BioTek Instruments, Inc.
UV/Vis spectrophotometer DU®530 Beckman Coulter, Inc.
CE N T R I F U G E S, I N C U B A T OR S, S H A K E R S, A N D L A M I N A R F L O W S
centrifuge producer
Avanti® J‐20 + JA‐10, JA‐18, and JS‐4.3 rotors Beckman Coulter, Inc.
Centrifuge 5415 D Eppendorf AG
Concentrator 5301 Eppendorf AG
Hettich Universal 32R Hettich Lab Technology GmbH
Hettich Rotanta 3500 Hettich Lab Technology GmbH
OptimaTMMAX ultracentrifuge + MLA‐80, TLA‐55 rotors Beckman Coulter, Inc.
UniVapo 150 ECH + Vaccucenter vacuum pump UniEquip GmbH
incubator / shaker producer / supplier
CAT RM 5 roller mixer CAT M. Zipperer GmbH
Duomax 1030 Heidolph GmbH & Co. KG
Gyrotory® water bath shaker (model G76) New Brunswick Scientific GmbH Heraeus® Function Line incubator Heraeus Instruments GmbH Innova 4230 refrigerated benchtop incubator shaker New Brunswick Scientific GmbH Innova CO‐170 CO2 incubator New Brunswick Scientific GmbH LD79 test‐tube rotator Labinco laboratory equipment
OT H E R E Q U I P M E N T
device, glassware, resource producer / supplier
accu‐jet® pro Brand®GmbH & Co. KG
Assistant® glass test tubes (100x16 mm) Karl Hecht GmbH & Co KG Barnstead™ E‐Pure™ Ultrapure Water Purification Systems Thermo Fisher Scientific Inc.
Cell Homogenizer with 10 μm clearance Isobiotech
Cellometer Auto T4 Plus PeqLab Biotechnologie GmbH
CoolCell® LX BioCision LLC.
CriterionTM cell Bio‐Rad Laboratories GmbH
DURAN® baffled conical flasks (50‐1000 ml) Schott AG DURAN® beaker glasses (50‐2000 ml) Schott AG DURAN® conical flasks (100‐300 ml) Schott AG DURAN® glass bottles (50‐2000 ml) Schott AG
GelAirTM Drying System Bio‐Rad Laboratories GmbH
GenePulser II Bio‐Rad Laboratories GmbH
Harvard Pump 11 Plus Single Syringe Harvard Apparatus Kimax® baffled conical flasks (250 ml) Kimble Chase LLC.
Luer Lock gas tight syringes (1 ml) Supelco®Co. LLC.
μMACS Separator Miltenyi Biotec GmbH
MagnaRackTM Magnetic Separation Rack InvitrogenTM Microman® pipettes (10 μl, 100 μl, 1000 μl) Gilson®AG
Mini‐PROTEAN® cell Bio‐Rad Laboratories GmbH
Mini‐Sub® Cell GT cell Bio‐Rad Laboratories GmbH
Multipette® Plus dispenser Eppendorf AG
Multipette® Xstream electronic dispenser Eppendorf AG
PERMAQ® Compact 270 reverse osmosis BTW Wassertechnik GmbH Pipetman® Classic pipettes (10 μl, 20 μl, 200 μl, 1000 μl) Gilson®AG
Positive Pressure‐96 Processor + 96‐Well Tabless Tube Holder
Waters GmbH Phenomenex®, Inc.
PowerPacTM 200 Power Supply Bio‐Rad Laboratories GmbH PowerPacTM 300 Power Supply Bio‐Rad Laboratories GmbH PowerPacTM 3000 Power Supply Bio‐Rad Laboratories GmbH Precellys® 24 tissue homogenizer
+ Cryolys temperature controller
Bertin Technologies SAS Bertin Technologies SAS Quartz SUPRASIL cuvette, 10 mm path (3500μl) Hellma®GmbH & Co. KG Quartz SUPRASIL UV/Vis cuvettes, 10 mm path (400μl) Hellma®GmbH & Co. KG RoboCycler® 96 thermal cycler Stratagene®
Scotsman AF80 ice flaker Scotsman Ice Systems Frimont S.P.A.
Sub‐Cell® GT Cell Bio‐Rad Laboratories GmbH
Trans‐Blot® SD semi‐dry transfer cell Bio‐Rad Laboratories GmbH
Ultrasonic cleaner (bath) VWR®LLC.
VacElut 20 Manifold
II.12.11. C
ONSUMABLESconsumable producer / supplier
12 % Criterion TGX precast midi gels Bio‐Rad Laboratories GmbH
96 DeepWellTM PP plates (MS) Nunc®
96‐well plates (PCR) + strips of 8 domed caps Thermo Scientific Capillary Pistons for Microman® (10 μl, 100μl, 1000μl) Gilson®AG
Cellometer counting chambers Nexcelom Bioscience LLC.
CELLSTAR® cell culture flasks (25 cm2, 75 cm2) Greiner Bio‐One Internat. GmbH centrifuge tubes (15 ml, 50 ml) Sarstedt AG & Co.
Combitips / advanced (100 μl – 25 ml) Eppendorf AG
cover slips (22x22 mm, 24x24 mm, 24x60 mm) Carl Roth®GMBH + CO. KG
Crimp μ‐vials (350 μl) Glastechnik Gräfenroda GmbH
CryoTubes (1.8 ml) Nunc®
Diamond® tips TowerpackTM (10 μl, 200μl, 1000μl) Gilson®AG Eppendorf tubes (1.5 ml, 2 ml, 5 ml) Eppendorf AG F96 MicroWellTM white polystyrene plates Nunc®
Fast‐Read 102® counting chambers Biosigma
Gas permeable seals (adhesive plate seal) Thermo Scientific
GelAirTM cellophane Bio‐Rad Laboratories GmbH
Gel Blotting Paper GB003 Whatman®
GenePulser/MicroPulser 0.2 cm gap electroporation cuvettes Bio‐Rad Laboratories GmbH
glass vials (2000 μl) Agilent Technologies, Inc.
Immobilon® FL PVDF membrane Merck Millipore micro vials PP (1.1 ml, 32 x 12 mm) with cap PU Zinsser Analytic GmbH MicroWellTM 96‐Well optical‐bottom plates Nunc®
MN Square‐well blocks (96 wells, 2.1 ml) Macherey‐Nagel GmbH & Co. KG Falcon® MultiwellTM plates (6‐well, 12‐well, 24‐well) BD Biosciences
CELLSTAR® serological pipettes (5 ml, 10 ml, 25 ml, 50 ml) Greiner Bio‐One Internat. GmbH
Parafilm® M Bemis®Inc.
Precellys® ceramic kit 1.4/2.8 mm 2.0 ml tubes PeqLab Biotechnologie GmbH Quick‐Seal polypropylene tube (8 ml) Beckman Coulter, Inc.
semi‐micro disposable PS cuvettes Brand®GmbH & Co. KG Snap‐On Cap polypropylene tubes (1.5 ml) Beckman Coulter, Inc.
SuperFrost Plus microscope slides Thermo Scientific
TipOne filtered tips (10μl, 20 μl, 200 μl, 1000μl) Starlab International GmbH Tissue culture dishes, 96 x 21 mm TPP Techno Plastic Products AG TPP cell scrapers, 240 mm long TPP Techno Plastic Products AG