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100 101 102 103 104 105

CD83 6

0

Counts

0

0 3

3 3 0

3 3 3 0 0

100 101 102 103 104 105

HLADR 19

0

0

Counts

0 10 10 10 0

10 10 10 0 0

100 101 102 103 104 105

CD86 9

0

C o u n ts 0

0 5 5 5 0

5 5 5 0 0

100 101 102 103 104 105

9 0

C o u n ts 0

0 5 5 5 0

5 5 5 0 0

Isotype Unprimed DCs

250ng SPAG9 DCs 500ng SPAG9 DCs

750ng SPAG9 DCs 1000ng SPAG9 DCs 96.96%

97.96%

97.5%

95.9%

97.85%

Isotype Unprimed DCs

250ng SPAG9 DCs 500ng SPAG9 DCs

750ng SPAG9 DCs 1000ng SPAG9 DCs 79.91%

87.9%

88.4%

91.6%

86.04%

CD80

Isotype Unprimed DCs

250ng SPAG9 DCs 500ng SPAG9 DCs

750ng SPAG9 DCs 1000ng SPAG9 DCs 92.02%

89.94%

88.56%

91.41%

92.38%

Isotype Unprimed DCs

250ng SPAG9 DCs 500ng SPAG9 DCs

750ng SPAG9 DCs 1000ng SPAG9 DCs

a b

100 101 102 103 104 105

CD40 24

0

0

Counts

0 12 12 12 0

12 12 12

0 0

Isotype Unprimed DCs

250ng SPAG9 DCs 500ng SPAG9 DCs

750ng SPAG9 DCs 1000ng SPAG9 DCs 95.9%

94.93%

94.09%

94.96%

96.95%

c

d e

Supplementary figure 1

84.39%

92.47%

93.65%

93.13%

93.8%

(2)

Supplementary figure 2

1.1x109 2.1x109 3.2x109 4.3x109

S S C

0

4.3x109

3.2x109

2.1x109

1.1x109

FSC

Unstained

Secondary control

SPDCs – 49.27%

a b

100 101 102 103 104 105

C o u n t

0

716

FL8-LOG_Height 537

358 179 0 537 358 179 0 537 358 179

CCR7

P7 P8 P9 P10

% o f ex p re s si o n

0

60

40

20

FoxP3+ FMO

204 153 102 51

0 51 102 153 204 256

SSC-Height

0

FSC-Height 256

192 128 64

100 101 102 103 104 105

SSC-Height

0

CD4-Log_Height Comp 256

102 101

CD25-Log_Height Comp

100

FOXP3-Log_Height Comp 100 101 102 103 104 105 103

104 105

c

204 153 102 51

100 101 102 103 104 105

SSC-Height

0

LVD-Log_Height Comp 256

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