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Supplementary materials

Supplementary Figure 1. Faecal samples mixed and resuspended in 1 ml of saline buffer (left) resulted in a better homogenisation of the stool as compared to using AVL buffer (right).

Supplementary Figure 2. CT values obtained with serial dilutions of stool using the N1 or N2 primer sets.

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Supplementary Table 1. Ct values obtained from stool samples spiked with different amounts of viral particles used to extract RNA with or without ultrafiltration.

Without ultrafiltration With ultrafiltration

Spiked viral particles (vp in 100 mg) N1 N1 N2 N2 N1 N1 N2 N2

2900 32.3 31.9 31.4 31.3 nt nt nt nt

1450 32.6 33.6 - - 32.7 32.6 31.3 31.6

725 nt nt nt nt 33.2 32.5 33.5 33.6

nt = not tested

- = Sample showed no Ct value

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