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Supplementary Figure 1. Positions of primers used in this study

Primer name Primer sequences (5’  3’)

scEDIII-F1 GAAGGATCCATGAAAGGAATGTCTTACGCA scE3_MIGH_F1_J TTTAAAAAGGGTTCCTCAGCTTCATCTACAAAAGTG

scEDIII_R2 CACTTTTGTAGATGAAGCTGAGGAACCCTTTTT

MIGHG2a_B2_Sal GTCGACTTAGTAGCAAGTGCCA

Supplementary Table 1. Primer information

*Underlines indicate the restriction enzyme sites.

Supplementary Table 2. Band intensity in Western blots (as measured by ImageJ 2) of total protein (reducing condition) and polymeric protein (non-reducing

condition)

Sample name Reducing condition Non-reducing condition Positive control 10,556

Sample 1 Day 1 4,824 28,785

Sample 1 Day 3 3,682 17,044

Sample 1 Day 5 2,621 8,734

Sample 2 Day 1 4,662 23,561

Sample 2 Day 3 3,460 9,039

Sample 2 Day 5 2,683 5,108

Reference:

Rueden CT, Schindelin J, Hiner MC, DeZonia BE, Walter AE, Arena ET, Eliceiri KW (2017) ImageJ2: ImageJ for the next generation of scientific image data. BMC Bioinformatics 529.

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