A
sh-NCsh-circ#1
sh-circ#2 sh-circ#3
DLD-1 LoVo
0.0 0.5 1.0 1.5
Relativeexpressionofcirc
*** *** *** ***
ns ns
DLD-1 LoVo
0.0 0.5 1.0 1.5
Relativeexpression ofGALNT16 mRNA
sh-NC sh-circ#1
sh-circ#2 sh-circ#3
ns ns ns ns ns
ns
B
RKO HCT 116
0.0 0.5 1.0 1.5
vector circ-GALNT16 ns
ns
Relativeexpression ofGALNT16 mRNA
RKO HCT 116
0 2 4 6 8 10
Relativeexpressionofcirc
vector circ-GALNT16
** **
C
0.0 0.5 1.0 1.5
RKO
Percentageoftotal
Cytoplasm Nucleus
U6 GAPDH
18S circ-GALNT16
0.0 0.5 1.0 1.5
HCT 116
Percentageoftotal
Cytoplasm Nucleus
U6 GAPDH
18S circ-GALNT16
E
Days sh-NC sh-circ-GALNT16
0 5 10 15 20 25 0.0
0.5 1.0 1.5 2.0
DLD-1
TumorVolume(cm3) **
vector circ-GALNT16
0 5 10 15 20 25 0.0
0.5 1.0 1.5 2.0
RKO
TumorVolume(cm3)
**
Days
Ki67 Serpine1
sh-NC
sh-circ
vector
circ
C-myc 50μM
DLD-1RKO