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Two Simple Methods for Measuring Isoprene Emission of Leaves by UV-Spectroscopy and GC-MS

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Two Simple Methods for Measuring Isoprene Emission of Leaves by UV-Spectroscopy and GC-MS

Johannes G. Zeidler and Hartmut K. Lichtenthaler

Botanical Institute II, Universityof Karlsruhe, D-76128 Karlsruhe, Germany

Z. Naturforsch.53 c,1087Ð1089 (1998);

received October 5, 1998

Isoprene,Platanus¥acerifolia, Quercus roburL., Robinia pseudoacaciaL., UV-Spectroscopy

Two new headspace methods for the analysis of iso- prene emission from detached plant leaves are de- scribed. The first method is based on the UV-absorption of isoprene emitted byleaves inside quartz cuvettes and can be used for the quantitative spectrophotometric de- termination of isoprene production. The second tech- nique is a micro-extraction method of isoprene from the cuvette air for GC analysis, and is very suitable for the determination of isotope-labeled isoprene byGC-MS.

Reprint requests to Prof. Dr. Dr. h. c. H. K. Lichtenthaler. Fax: +49 721 608 3874.

E-mail: hartmut.lichtenthaler@bio-geo.uni-karlsruhe.de

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