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Supplementary Material Impacts of ocean acidification on the development of a subtropical zooplankton community during oligotrophic conditions and simulated upwelling

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Supplementary Material

Impacts of ocean acidification on the development of a subtropical zooplankton community during oligotrophic conditions and simulated upwelling

María Algueró-Muñiz

*, Henriette G. Horn

, Santiago Alvarez-Fernandez

1

, Carsten Spisla

1,2

, Nicole Aberle

4

, Lennart T. Bach

2

, Wanchun Guan

3

, Eric P. Achterberg

2

, Ulf Riebesell

2

, Maarten Boersma

1,5

*Corresponding author: maria.alguero@awi.de

(please see below)

(2)

Supplementary Table 1: Biomass conversion factors. Only common species (species that represent >0.5% total catch (i.e. > 3540 ind), t1-t55) were considered for mesozooplankton biomass estimation. Carbon content was estimated for Doliolum sp. and Oncaea sp. from last sampling day (t56) samples.

MESOZOOPLANKTON Conversion factor

(µg C ind-1) Reference Clausocalanus

spp./Paracalanus spp. 0.339 averaged for Paracalanus sp. after Uye (2014)

Copepoda nauplii 0.04 length-carbon relationship for Oithona similis, from Sabatini & Kiørboe (1994)

Doliolum sp. 2.18 this study

Foraminifera 0.75 average for Elphidium (Moodley 2000)

Microsetella spp. 0.268 averaged for Microsetella norvegica after Uye (2014)

Nannocalanus minor 0.339 based on Clausocalanus spp./Paracalanus spp.

Oikopleura dioica;

juveniles 1.178 averaged for juveniles ~500 µm trunk length

after King (1980)

Oithona spp. 0.58 Kiørboe & Sabatini (1994)

Oncaea spp. 2.7 this study

MICROZOOPLANKTON Conversion factor

Ciliates 0.76 V0.819 pg C cell-1 Menden-Deuer & Lessart (2000) Dinoflagellates 0.19 pg C mL-1 Putt & Stoecker (1989)

PHYTOPLANKTON Conversion factor (pg C cell-1)

Dinoflagellates 0.76 V0.819 Menden-Deuer & Lessart (2000) Diatoms 0.288 V0.811 Menden-Deuer & Lessart (2000) Diverse 0.216 V0.939 Menden-Deuer & Lessart (2000)

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