WT KI WT KI WT KI WT Suppl. Figure 1
Kir2.1
GAPDH
S1. Kir2.1 expression in isolated ventricular cardiomyocytes of WT and KI mice.
A) Original western blot for the analysis of Kir2.1 expression. B) Analysis of mean densitometric values normalized to GADPH show a significant reduction of Kir2.1 expression in KI compared to WT myocytes (0.198±0.014 vs. 0.323±0.024 A.U., N=3 vs 4, * - p=0.0094, unpaired two-tailed Student´s t-test)
A B
Suppl. Figure 2
A B
S2.Take-off-potentials (A) and Time of reactivation (B) of early afterdepolarizations (EAD).
Neither take-off-potentials of early afterdepolarizations (A) (-42.62±1.32 vs -38.13±3.17 vs -46.23±5.93 vs -40.93±2.94 mV; n(cells)=6 vs 7 vs 4 vs 4, WT H2O2 4 min vs WT H2O2 8 min vs KI H2O2 4 min vs KI H2O2 8 min, p=n.s., one- way ANOVA with Holm-Sidak´s post hoc test) nor their times of reactivation (i.e.
EAD delay) (B) (38.22±2.47 vs 38.45±3.99 vs 37.16±3.63 vs 43.71±5.86 ms, n(cells)=6 vs 7 vs 3 vs 4, WT H2O2 4 min vs WT H2O2 8 min vs KI H2O2 4 min vs KI H2O2 8 min, p=n.s., one-way ANOVA with Holm-Sidak´s post hoc test) are different between the genotypes or significantly change within 8 minutes of H2O2 superfusion.
