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Additional Figure 1 A)B)

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Choroid Plexus Epithelial

Gdf11Ttr Neuroblast

Gdf11Dcx

Endothelial Pecam1

Gdf11

Additional Figure 1

Oligodendrocyte

& OPCs Pdgfra, Olig1

Gdf11

Microglia Itgam Gdf11 Endothelial

Pecam1 Gdf11

A) B)

(2)

A) B)

Additional Figure 2

Dentate Gyrus 0

1 2 3

puncta / cell

Gdf11 Mstn

Gdf11 Mstn

(3)

B) C)

YoungOld

A)

Dentate Gyrus CA1 CA3

Additional Figure 3

Gdf11 Alk4 Alk5 Tgfβ1

0.0 0.5 1.0 1.5 2.0

2-ΔΔCt

Young Old

ns

ns

ns ns

DG CA1 CA3

0 2 4 6 8 10 12 14

Gdf11puncta/cell OldYoung

ns ns

ns

(4)

Controls Gdf11cKO 0

5 10 15 20

Gdf11puncta / cell

Gdf11CAG-Creflox/floxER; Gdf11flox/flox Gdf11cKO

RNAscope

A) B)

qPCR

Additional Figure 4 Controls Gdf11cKO

0.0 0.5 1.0 1.5

2-ΔΔCt

Gdf11flox/flox

CAG-CreER; Gdf11flox/flox Gdf11cKO

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