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Figure 1: Digestion profile of a fragment (690 base pairs) of the amplified coding region of the mitochondrial gene of subunit 2 of NADH dehydrogenase, using PCR-RFLP. A)

Amplicon digested by restriction enzyme DdeI. Lines 1 and 2: N. rossii. Lines 3 and 4: N.

coriiceps Richardson, 1844. Lines 5 and 6: N. neglecta Nybelin, 1951; B) Amplicon

digested by restriction enzyme HindIII. Lines 1 and 2: N. rossii. Lines 3 and 4: N. coriiceps Richardson, 1844. Lines 5 and 6: N. neglecta Nybelin, 1951; C) Amplicon digested by restriction enzyme RsaI. Lines 1 and 2: N. coriiceps Richardson, 1844. Lines 3 and 4: N.

neglecta Nybelin, 1951. Lines 5 and 6: N. rossii. M: 1kb Plus Ladder (Invitrogen).

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Figure 1

Figure 1: Digestion profile of a fragment (690 base pairs) of the amplified coding region of the mitochondrial gene of subunit 2 of NADH dehydrogenase, using PCR-RFLP. A)..

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