Table S1.

(1)

Table S1. Taxon sampling and gene coverage as indicated by GenBank voucher numbers for phylogenetic analyses, including biogeographic range coding for ancestral state reconstruction in BioGeoBears. See Fig. 2 for which area the coded character represents.

Genus Subgenus species ^voucher

# locality data Biogeo

region COI COII 28S 12S AK H3

Aulono-

gyrus Afrogyrus altern-

atus MB525 see Miller & Bergsten, 2012 A MH029

976 MH029

949 JX4780

08.1 MH030

003 JX4779 27.1 Aulono-

gyrus Afrogyrus ater GTG20 Kenya: Meru Co., Mt. Kenya Nat’l Prk., Sirimon gate, 00.00568°S, 37.24656°E, 2613m, 17.vi.2013, leg. SM Baca, KB Miller, ex. forest stream, SMB170613-A.

A MH029

957 MH029

984 MH029

939 MH029

925 MH029

992 Aulono-

gyrus Afrogyrus bedeli MB493 see Miller & Bergsten, 2012 A JX4780

69.1 JX4781

57.1 MH029

953 JX4779

80.1 MH030

000 JX4779 00.1 Aulono-

gyrus Afrogyrus caffer GTG35 Tanzania: Kagera Reg. ca. 5km E of Bhiaramulo, 02°38.159’S, 31°20.789’E, 1427m, 26.vii.2010, leg. R.W.Sites & A.Mbogho. L-1154.

A JX4781

01.1 MH029

985 MH029

948 JX4780

131.1 MH030

001 MH029

991 Aulono-

gyrus Afrogyrus capen-

sis GTG8 South Africa: Western Cape Prov.

trib. strm. to Keurboom River crossing R339, 33.8612°S, 23.1729°E, 250m, 13.xii.2015, leg.

J.Bergsten, A.Desamore, K.B.Miller, RSA15-21.

A MH029

965 MH029

982 MH029

940 MH029

914 MH030

004 Aulono-

gyrus Afrogyrus Conspi-

cuus GTG18 Kenya: Meru Co., Mt. Kenya Nat’l Prk., Sirimon gate, 00.00568°S, 37.24656°E, 2613m, 17.vi.2013, leg. SM Baca, KB Miller, ex. forest stream, SMB170613-A.

A MH029

958 MH029

975 MH029

942 MH029

920 MH030

002 MH029

993 Aulono-

gyrus Afrogyrus depres-

sus GTG12 South Africa: Western Cape Prov.

Du Toit River under bridge road R45, 33.9482°S, 19.1689°E, 301m, 20.xii.2015, leg. J.Bergsten, A.Desamore, K.B.Miller, RSA15-48.

A MH029

966 MH029

983 MH029

941 MH029

915 MH030

005 Aulono-

gyrus Afrogyrus flavi-

pes GTG14 Tanzania: Kagera Reg. ca. 5km E of Bhiaramulo, 02°38.159’S, 31°20.789’E, 1427m, 26.vii.2010, leg. R.W.Sites & A.Mbogho. L-1154.

A MH029

955 MH029

943 MH029

999 Aulono-

gyrus Afrogyrus graueri GTG39 Kenya: Nyeri Co., SE Mt. Kenya, 00.37492°S, 37.15634°E, 2040m, 19.vi.2013, leg. SM Baca, KB Miller, ex. mountain stream, SMB190613- A.

A MH029

960 MH029

977 MH029

945 MH029

921 MH030

009 MH029

994 Aulono-

gyrus Afrogyrus hypox-

anthus GTG41 Kenya: Nyeri Co., SE Mt. Kenya, 00.37492°S, 37.15634°E, 2040m, 19.vi.2013, leg. SM Baca, KB Miller, ex. mountain stream, SMB190613- A.

A MH029

962 MH029

979 MH029

952 MH029

922 MH030

011 Aulono-

gyrus Afrogyrus knysna-

nus GTG10 South Africa: Western Cape Prov.

Jonkershoek Nat. Res. side of Jonkershoek trail, strm trib. To Eerste river, 33.9816°S, 18.9455°E, 291m, 20.xii.2015, leg. J.Bergsten, A.Desamore, K.B.Miller, RSA15-44.

A MH029

959 MH029

944 MH029

919 MH030

007 Aulono-

gyrus Afrogyrus margin

-atus MB523 see Miller & Bergsten, 2012 A JX4780

95.1 MH029

972 MH029

936 JX4780

06.1 JX4779

25.1 Aulono-

gyrus Afrogyrus navi-

culus GTG4 South Africa: Western Cape Prov.

Groot river, bridge, R102 by Nat.

Val. Restcamp, 33.9687°S, 23.5595°E, 10m, 9.xii.2015, leg.

J.Bergsten, A.Desamore, K.B.Miller, ex. forest river, RSA15-03.

A MH029

963 MH029

980 MH029

947 MH029

916 MH030

008 Aulono-

gyrus Afrogyrus seydeli GTG37 Kenya: Narok Co., Kilgoris-Lolgorian Rd., Migor River, 01.17307°S, 34.83674°E, 1581m, 13.vi.2013, leg. SM Baca, KB Miller, ex. shallow rocky stream, SMB130613-C.

A MH029

961 MH029

978 MH029

951 MH029

923 MH030

010 MH029

990

(2)

Aulono-

gyrus Afrogyrus sp. MB569 see Miller & Bergsten, 2012 A JX4781

06.1 JX4781

93.1 MH029

950 JX4780 18.1 Aulono-

gyrus Afrogyrus varians GTG2 South Africa: Western Cape Prov. Du Toit River under bridge road R45, 33.9482°S, 19.1689°E, 301m, 20.xii.2015, leg. J.Bergsten, A.Desamore, K.B.Miller, RSA15 -48.

A MH029

964 MH029

981 MH029

946 MH029

913 MH030

006 Aulono-

gyrus Aulono-

gyrus anti-

podum GTG65 New Caledonia: River Carénage, 22°16.529'S, 166°49.382'E, 191m, 18.i.2017, leg. N.Charpin, NC-011

O MH029

970 MH029

934 MH029

924 MH029

995 Aulono-

gyrus Aulono-

gyrus striatus MB469 see Miller & Bergsten, 2012 P JX4780

56.1 JX4781

44.1 JX4779

67.1 KX7755

22.1 JX4778 89.1 Aulono-

gyrus Aulono-

gyrus strigo-

sus MB687 see Miller & Bergsten, 2012 O JX4781

43.1 JX4782

23.1 MH029

935 JX4780 54.1 Aulono-

gyrus Lopho-

gyrus carini-

pennis MB604 see Miller & Bergsten, 2012 M JX4781

15.1 JX4782

02.1 JX4780

27.1 MH029

988 Aulono-

gyrus Lopho-

gyrus crista-

tus MB519 see Miller & Bergsten, 2012 M JX4780

91.1 JX4781

79.1 MH029

937 JX4780

02.1 MH029

998 JX4779 21.1 Aulono-

gyrus Paragyrus goudoti MB529 see Miller & Bergsten, 2012 M JX4780

99.1 JX4781

86.1 JX4780

11.1 JX4779

30.1 Aulono-

gyrus Ptery-

gyrus elegant -issi- mus

GTG6 Madagascar, Fianarantsoa, stream E Ranomofana, 21º16.532'S, 47º29.977'E, 13 Nov 2008, K.B.

Miller, leg. KBM13110801

M MH029

954 MH029

986 MH029

938 MH029

917 MH029

997 MH029

989 Dineu-

tus Cyclous sublin-

eatus GenBa nk

see Miller & Bergsten, 2012 - JX4780

85.1 JX4781

73.1 EU677

683.1 JX4779

96.1 KX7755

36.1 JX4779 15.1 Gyrinus Gyrinus maculi-

ventris MB839 see Gustafson & Miller, 2017 - KX775

621.1 KX7756

72.1 MH029

927 KX7755

13.1 KX7755 72.1 Gyrinus Gyrinus plicifer MB495 see Miller & Bergsten, 2012 - JX4780

70.1 JX4781

58.1 JX4779

81.1 JX4779

01.1 Gyrinus Oreo-

gyrinus dimor-

phus MB839 see Gustafson & Miller, 2017 - KX775

622.1 KX7756

73.1 MH029

933 KX7755

14.1 KX7755

73.1 KX7757 19.1 Gyrinus Neo-

gyrinus chaly-

baeus GTG25 Brazil: Mina Gerais, Serra do Caraça, 20.11615°S, 43.47670°W, 1260m, 31.i.2015 leg. M.V.P.

Simões & C. Assis.

- MH029

968 MH029

973 MH029

932 MH029

926 MH030

014 Gyrinus Neo-

gyrinus rozei GTG29 Venezuela: Bolivar, Gran Sabana, S.

Rio Sakaika, Rt 10, 5.57495°S, 61.31205°W, 1100m, 2.viii.2008, leg. A.E.Z.Short, roadside pond. AS- 08-067.

- MH029

969 MH029

974 MH029

930 MH029

912 MH030

012 Gyrinus Neo-

gyrinus ovatus MB492 see Miller & Bergsten, 2012 - JX4780

68.1 JX4781

56.1 MH029

931 JX4779

79.1 MH030 013 Gyrinus Gyrinulus minu-

tus MB526 see Miller & Bergsten, 2012 - JX4780

97.1 JX4781

84.1 JX4780

09.1 JX4779

28.1 Macro-

gyrus Macro-

gyrus oblong-

gus Gen-

Bank

see Gustafson & Miller, 2017 - KX775

600.1 KX7756 52.1 EU677

682.1 KX7754

92.1 KX7755

53.1 KX7757 00.1 Meta-

gyrinus sinen-

sis JLKB1 see Miller & Bergsten, 2012 S JX4781

39.1 JX4782 19.1 Orecto-

chilus dauri-

cus GTG62 Mongolia: Hovsgol Aimag, Mörön Soum, Tunamal Nuur, 5 km W of Arbulag, 49.89999°N, 99.39450°E, 1871m, 06.vii.2006, leg. P.J.Torres.

SRP06070603.

- MH029

971 MH029

928 MH029

911 MH02

9987

Porro-

rhynchus landaisi Gen-

Bank

see Gustafson & Miller, 2017 - KX775

618.1 KX7756

69.1 KX7755

10.1 KX77556

9.1 KX775

717.1 Patrus produc

-tus Gen- Bank

see Miller & Bergsten, 2012 - JX4780

78.1 JX4781

66.1 JX4779

90.1 KX77557

6.1 JX477

909.1

(3)

Table S2. Primers used for amplification and sequencing.

Gene Forward primer (5’-3’) Reverse primer (5’-3’) Reference

COI Jerry, CAACATTTATTTTGATTTTTTGG Pat, TCCAATGCACTAATCTGCCATATTA S IMON et al.

(1994) COII F-leu, TCTAATATGGCAGATTAGTGC R-lys, GAGACCAGTACTTGCTTTCAGTCATC W HITING

(2002) 12S 12Sai, AAACTACGATTAGATACCCTATTAT 12Sbi, AAGAGCGACGGGCGATGTGT

S VENSON &

W HITING (2004) 28S NLF184-21, ACCCGTGAAYTTAAGCATAT LS1041R, TACGGACRTCCATCAGGGTTTCCCCTGATTC

W ILD &

M ADDISON (2008) H3 HexAF, ATGGCTCGTACCAAGCAGACGGC HexAR,, ATATCCTTGGGCATGATGGTGAC C OLGAN et

al. (1998) AK AK183F, GATTCTGGAGTCGGNATYTAYGCNCCYGAYGC AK939R, GCCNCCYTCRGCYTCRGTGTGYTC

W ILD &

M ADDISON (2008) AK GyrAKF1, CAAGAAGACTGACAAGCATCC GyrAKR1, CACCTGCAAGTTGAACTT This study

Internal primers were designed for AK183F and AK939R, these are GyrAKF1 and GyrAKR1. Nested PCR was performed using these primers to amplify Arginine kinase in difficult specimens.

References

C OLGAN D.J., M C L AUCHLAN A., W ILSON G.D.F., L IVINGSTON S.P., E DGECOMBE G.D., M ACARANAS J.,

ET AL . 1998. Histone H3 and U2 snRNA DNA sequences and arthropod molecular evolution. – Australian Journal of Zoology 46: 419–437.

S IMON C., F RATI F., B ECKENBACH A., C RESPI B., L IU H., F LOOK P. 1994. Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. – Annals of the Entomological Society of America 87: 651– 701.

S VENSON G.J., W HITING M.F. 2004. Phylogeny of Mantodea based on molecular data: Evolution of a charismatic predator. – Systematic Entomology 29: 359– 370.

W HITING M.F. 2002. Mecoptera is paraphyletic: multiple genes and phylogeny of Mecoptera and Siphonapter a . – Zoologica Scripta 31: 93– 104.

W ILD A.L., M ADDISON D.R. 2008. Evaluating nuclear protein-coding genes for phylogenetic utility in beetles. – Molecular Phylogenetics and Evolution 48: 877– 891.

Table S3. Dispersal rate multipliers used in BioGeoBEARS ancestral range reconstruction.

A M P S O

1.00 0.50 0.75 0.25 0.10 0.50 1.00 0.10 0.10 0.10 0.75 0.10 1.00 0.75 0.25 0.10 0.10 0.75 1.00 0.75 0.10 0.10 0.25 0.75 1.00

A M P S O

1.00 0.50 0.50 0.10 0.10 0.50 1.00 0.10 0.10 0.10 0.50 0.10 1.00 0.75 0.10 0.10 0.10 0.75 1.00 0.10 0.10 0.10 0.10 0.10 1.00

A M P S O

1.00 0.50 0.25 0.10 0.10

0.50 1.00 0.10 0.75 0.25

0.25 0.10 1.00 0.50 0.10

0.10 0.75 0.50 1.00 0.10

0.10 0.25 0.10 0.10 1.00

(4)

0 . 2

GTG29_Gyrinus_rozei

GTG65_Aulonogyrus_antipodum

GTG37_Aulonogyrus_seydeli

MB604_Aulonogyrus_carinipennis MB839_Gyrinus_dimorphus

MB469_Aulonogyrus_striatus

GTG39_Aulonogyrus_graueri MB492_Gyrinus_ovatus

GTG25_Gyrinus_chalybaeus

MB523_Aulonogyrus_marginatus

GTG41_Aulonogyrus_hypoxanthus

GTG6_Aulonogyrus_elegantissimus

JLKB1_Metagyrinus_sinensis

GTG2_Aulonogyrus_varians Patrus_productus

MB569_Aulonogyrus_sp

GTG4_Aulonogyrus_naviculus Porrorhynchus_landaisi

GTG18_Aulonogyrus_conspicuus MB526_Gyrinus_minutus

GTG10_Aulonogyrus_knysnanus

GTG8_Aulonogyrus_capensis Dineutus_sublineatus

GTG12_Aulonogyrus_depressus GTG35_Aulonogyrus_caffer MB495_Gyrinus_plicifer

MB493_Aulonogyrus_bedeli

MB529_Aulonogyrus_goudoti GTG20_Aulonogyrus_ater

MB519_Aulonogyrus_cristatus

MB525_Aulonogyrus_alternatus GTG62_Orectochilus_dauricus

MB837_Gyrinus_maculiventris Macrogyrus_oblongus

MB687_Aulonogyrus_strigosus

GTG14_Aulonogyrus_flavipes 9 3

6 9

1 0 0 9 9

9 6

1 0 0 3 7

8 0

8 3 8 8

6 1 1 0 0

9 7

1 0 0 9 1

1 0 0 7 6

5 0

1 0 0

6 0 5 9 8 9

1 0 0 1 0 0

1 0 0

1 0 0 9 7

9 1 1 0 0

9 8 9 8

7 2

F i g u r e S 1 . R e s u l t s o f m a x i m u m l i k e l i h o o d

p h y l o g e n e t i c a n a l y s i s u s i n g I Q - T r e e . N u m b e r s a t

n o d e s i n d i c a t e s u p e r f a s t b o o t s t r a p s u p p o r t .

(5)

0 . 2

MB469_Aulonogyrus_striatus

MB687_Aulonogyrus_strigosus GTG25_Gyrinus_chalybaeus

GTG65_Aulonogyrus_antipodum

GTG12_Aulonogyrus_depressus GTG4_Aulonogyrus_naviculus MB495_Gyrinus_plicifer

GTG2_Aulonogyrus_varians MB526_Gyrinus_minutus

MB839_Gyrinus_dimorphus

GTG37_Aulonogyrus_seydeli MB523_Aulonogyrus_marginatus

GTG20_Aulonogyrus_ater GTG29_Gyrinus_rozei

GTG41_Aulonogyrus_hypoxanthus

GTG8_Aulonogyrus_capensis Dineutus_sublineatus

JLKB1_Metagyrinus_sinensis

GTG10_Aulonogyrus_knysnanus

MB569_Aulonogyrus_sp GTG39_Aulonogyrus_graueri

GTG18_Aulonogyrus_conspicuus GTG6_Aulonogyrus_elegantissimus MB837_Gyrinus_maculiventris

MB604_Aulonogyrus_carinipennis

GTG14_Aulonogyrus_flavipes Porrorhynchus_landaisi

MB525_Aulonogyrus_alternatus GTG62_Orectochilus_dauricus

MB529_Aulonogyrus_goudoti GTG35_Aulonogyrus_caffer

Macrogyrus_oblongus

MB493_Aulonogyrus_bedeli

Patrus_productus

MB519_Aulonogyrus_cristatus MB492_Gyrinus_ovatus

7 9 1 0 0 1 0 0

1 0 0

6 8

9 1 8 8

1 0 0

9 9

1 0 0

1 0 0 1 0 0

8 5

9 8

1 0 0

9 9 1 0 0

9 8

1 0 0 6 4 9 9

1 0 0 1 0 0

8 8

1 0 0

5 0

1 0 0 6 2

9 7 1 0 0

1 0 0 1 0 0

1 0 0

F i g u r e S 2 . R e s u l t s o f t i m e - f r e e B a y e s i a n

p h y l o g e n e t i c i n f e r e n c e u s i n g M r B a y e s . N u m b e r s

a t n o d e s i n d i c a t e p o s t e r i o r p r o b a b i l i t y s u p p o r t .

(6)

- 2 5 0 2 0 0 1 5 0 1 0 0 5 0 0

GTG8_Aulonogyrus_capensis GTG29_Gyrinus_rozei Macrogyrus_oblongus

GTG65_Aulonogyrus_antipodum

GTG39_Aulonogyrus_graueri MB839_Gyrinus_dimorphus GTG62_Orectochilus_dauricus

GTG37_Aulonogyrus_seydeli JLKB1_Metagyrinus_sinensis

MB493_Aulonogyrus_bedeli Porrorhynchus_landaisi Dineutus_sublineatus

GTG10_Aulonogyrus_knysnanus

GTG12_Aulonogyrus_depressus Patrus_productus

GTG14_Aulonogyrus_flavipes

GTG20_Aulonogyrus_ater MB837_Gyrinus_maculiventris MB495_Gyrinus_plicifer

GTG41_Aulonogyrus_hypoxanthus MB469_Aulonogyrus_striatus

MB523_Aulonogyrus_marginatus

GTG4_Aulonogyrus_naviculus GTG18_Aulonogyrus_conspicuus

GTG2_Aulonogyrus_varians MB519_Aulonogyrus_cristatus

MB569_Aulonogyrus_sp

MB525_Aulonogyrus_alternatus GTG35_Aulonogyrus_caffer MB687_Aulonogyrus_strigosus

GTG6_Aulonogyrus_elegantissimus

MB529_Aulonogyrus_goudoti MB604_Aulonogyrus_carinipennis MB492_Gyrinus_ovatus

MB526_Gyrinus_minutus

GTG25_Gyrinus_chalybaeus

1 0 0 1 0 0

1 0 0

1 0 0 1 0 0

9 9 7 2

9 1 1 0 0

1 0 0

1 0 0 9 9

7 4

6 1

1 0 0 1 0 0

1 0 0

1 0 0 9 4 6 0

1 0 0 1 0 0

1 0 0

1 0 0 9 9

1 0 0

1 0 0 7 3

7 2 9 6

1 0 0 1 0 0

1 0 0

7 9 9 8

F i g u r e S 3 . R e s u l t s o f t i m e - c a l i b r a t e d

B a y e s i a n p h y l o g e n e t i c i n f e r e n c e u s i n g a

n o d e - c a l i b r a t e d a p p r o a c h u n d e r t h e F B D m o d e l

i n M r B a y e s . N u m b e r s a t n o d e s i n d i c a t e

p o s t e r i o r p r o b a b i l i t y s u p p o r t . C o l o r e d b a r s

s h o w 9 5 % h y p e r p r i o r d i s t r i b u t i o n f o r a g e .

(7)

GTG6_Aulonogyrus_elegantissimu MB604_Aulonogyrus_carinipennis MB519_Aulonogyrus_cristatus GTG14_Aulonogyrus_flavipes MB493_Aulonogyrus_bedeli GTG35_Aulonogyrus_caffer MB529_Aulonogyrus_goudoti GTG20_Aulonogyrus_ater GTG18_Aulonogyrus_conspicuus MB525_Aulonogyrus_alternatus GTG39_Aulonogyrus_graueri MB569_Aulonogyrus_sp GTG37_Aulonogyrus_seydeli GTG41_Aulonogyrus_hypoxanthus GTG4_Aulonogyrus_naviculus GTG2_Aulonogyrus_varians GTG8_Aulonogyrus_capensis GTG12_Aulonogyrus_depressus GTG10_Aulonogyrus_knysnanus MB523_Aulonogyrus_marginatus MB469_Aulonogyrus_striatus GTG65_Aulonogyrus_antipodum MB687_Aulonogyrus_strigosus JLKB1_Metagyrinus_sinensis

70 60 50 40 30 20 10 0

Millions of years ago AS

A

M

M A

A

A A

A A A

A A

A A A

O

M M M A A A M A A A A A A A A A A A A A P O O S BioGeoBEARS DEC on Aulonogyrus M0_unconstrained ancstates: global optim, 3 areas max. d=0.0111; e=0.1657; j=0; LnL=−15.69

S

A

M

M A

A

A A

A

A A

A A A

O

A

M

M A

A

A A

A A A

A A

A A A

O

Figure S4.

(8)

70 60 50 40 30 20 10 0

Millions of years ago

M M M A A A M A A A A A A A A A A A A A P O O S BioGeoBEARS DEC on Aulonogyrus M0_unconstrained ancstates: global optim, 3 areas max. d=0.0111; e=0.1657; j=0; LnL=−15.69

Figure S5.

(9)

70 60 50 40 30 20 10 0

Millions of years ago AS

A

M

M A

A

A A

A A A

A A

A A A

O

M M M A A A M A A A A A A A A A A A A A P O O S BioGeoBEARS DEC+J on Aulonogyrus M0_unconstrained ancstates: global optim, 3 areas max. d=0.0123; e=0.4895; j=0; LnL=−15.01

S

A

M

M A

A

A A

A

A A

A A A

O

A

M

M A

A

A A

A A A

A A

A A A

O

Figure S6.

(10)

70 60 50 40 30 20 10 0

Millions of years ago

M M M A A A M A A A A A A A A A A A A A P O O S BioGeoBEARS DEC+J on Aulonogyrus M0_unconstrained ancstates: global optim, 3 areas max. d=0.0123; e=0.4895; j=0; LnL=−15.01

Figure S7.

(11)

70 60 50 40 30 20 10 0

Millions of years ago ASO

AO

A

M

M A

A

A A

A A A

A A

A A PO

O

M M M A A A M A A A A A A A A A A A A A P O O S BioGeoBEARS DIVALIKE on Aulonogyrus M0_unconstrained ancstates: global optim, 3 areas max. d=1e−04; e=1e−04; j=0.0648; LnL=−16.47

S

PO

A

M

M A

A

A A

A

A A

A A O

O

AO

A

M

M A

A

A A

M A

A A

A A A

A A

A A P

O

Figure S8.

(12)

70 60 50 40 30 20 10 0

Millions of years ago

M M M A A A M A A A A A A A A A A A A A P O O S BioGeoBEARS DIVALIKE on Aulonogyrus M0_unconstrained ancstates: global optim, 3 areas max. d=1e−04; e=1e−04; j=0.0648; LnL=−16.47

Figure S9.

(13)

70 60 50 40 30 20 10 0

Millions of years ago ASO

AO

A

M

M A

A

A A

A A A

A A

A A PO

O

M M M A A A M A A A A A A A A A A A A A P O O S BioGeoBEARS DIVALIKE+J on Aulonogyrus M0_unconstrained ancstates: global optim, 3 areas max. d=0.001; e=1e−04; j=0.0275; LnL=−17.53

S

PO

A

M

M A

A

A A

A

A A

A A O

O

AO

A

M

M A

A

A A

M A

A A

A A A

A A

A A P

O

Figure S10.

(14)

70 60 50 40 30 20 10 0

Millions of years ago

M M M A A A M A A A A A A A A A A A A A P O O S BioGeoBEARS DIVALIKE+J on Aulonogyrus M0_unconstrained ancstates: global optim, 3 areas max. d=0.001; e=1e−04; j=0.0275; LnL=−17.53

Figure S11.

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Millions of years ago ASO

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M M M A A A M A A A A A A A A A A A A A P O O S BioGeoBEARS BAYAREALIKE on Aulonogyrus M0_unconstrained ancstates: global optim, 3 areas max. d=0.0049; e=0.0149; j=1e−04; LnL=−29.20

ASO

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Figure S12.

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Millions of years ago

M M M A A A M A A A A A A A A A A A A A P O O S BioGeoBEARS BAYAREALIKE on Aulonogyrus M0_unconstrained ancstates: global optim, 3 areas max. d=0.0049; e=0.0149; j=1e−04; LnL=−29.20

Figure S13.

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Millions of years ago S

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M M M A A A M A A A A A A A A A A A A A P O O S BioGeoBEARS BAYAREALIKE+J on Aulonogyrus M0_unconstrained ancstates: global optim, 3 areas max. d=0; e=0; j=0.1013; LnL=−21.45

S

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Figure S14.

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Millions of years ago

M M M A A A M A A A A A A A A A A A A A P O O S BioGeoBEARS BAYAREALIKE+J on Aulonogyrus M0_unconstrained ancstates: global optim, 3 areas max. d=0; e=0; j=0.1013; LnL=−21.45

Figure S15.

Table S1.

Table S1. Taxon sampling and gene coverage as indicated by GenBank voucher numbers for phylogenetic analyses, including biogeographic range coding for ancestral state reconstruction in BioGeoBears. See Fig. 2 for which area the coded character represents.

Genus Subgenus species voucher

# locality data Biogeo

region COI COII 28S 12S AK H3

Aulono-

gyrus Afrogyrus altern-

atus MB525 see Miller & Bergsten, 2012 A MH029

976 MH029

949 JX4780

08.1 MH030

003 JX4779 27.1 Aulono-

gyrus Afrogyrus ater GTG20 Kenya: Meru Co., Mt. Kenya Nat’l Prk., Sirimon gate, 00.00568°S, 37.24656°E, 2613m, 17.vi.2013, leg. SM Baca, KB Miller, ex. forest stream, SMB170613-A.

A MH029

957 MH029

984 MH029

939 MH029

925 MH029

992

Aulono-

gyrus Afrogyrus bedeli MB493 see Miller & Bergsten, 2012 A JX4780

69.1 JX4781

57.1 MH029

953 JX4779

80.1 MH030

000 JX4779 00.1 Aulono-

gyrus Afrogyrus caffer GTG35 Tanzania: Kagera Reg. ca. 5km E of Bhiaramulo, 02°38.159’S, 31°20.789’E, 1427m, 26.vii.2010, leg. R.W.Sites & A.Mbogho. L-1154.

A JX4781

01.1 MH029

985 MH029

948 JX4780

131.1 MH030

001 MH029

991

Aulono-

gyrus Afrogyrus capen-

sis GTG8 South Africa: Western Cape Prov.

trib. strm. to Keurboom River crossing R339, 33.8612°S, 23.1729°E, 250m, 13.xii.2015, leg.

J.Bergsten, A.Desamore, K.B.Miller, RSA15-21.

A MH029

965 MH029

982 MH029

940 MH029

914 MH030

004

Aulono-

gyrus Afrogyrus Conspi-

cuus GTG18 Kenya: Meru Co., Mt. Kenya Nat’l Prk., Sirimon gate, 00.00568°S, 37.24656°E, 2613m, 17.vi.2013, leg. SM Baca, KB Miller, ex. forest stream, SMB170613-A.

A MH029

958 MH029

975 MH029

942 MH029

920 MH030

002 MH029

993

Aulono-

gyrus Afrogyrus depres-

sus GTG12 South Africa: Western Cape Prov.

Du Toit River under bridge road R45, 33.9482°S, 19.1689°E, 301m, 20.xii.2015, leg. J.Bergsten, A.Desamore, K.B.Miller, RSA15-48.

A MH029

966 MH029

983 MH029

941 MH029

915 MH030

005

Aulono-

gyrus Afrogyrus flavi-

pes GTG14 Tanzania: Kagera Reg. ca. 5km E of Bhiaramulo, 02°38.159’S, 31°20.789’E, 1427m, 26.vii.2010, leg. R.W.Sites & A.Mbogho. L-1154.

A MH029

955 MH029

943 MH029

999

Aulono-

gyrus Afrogyrus graueri GTG39 Kenya: Nyeri Co., SE Mt. Kenya, 00.37492°S, 37.15634°E, 2040m, 19.vi.2013, leg. SM Baca, KB Miller, ex. mountain stream, SMB190613- A.

A MH029

960 MH029

977 MH029

945 MH029

921 MH030

009 MH029

Genus Subgenus species ^voucher