Table S1. Haplotype composition (GenBank accession number) for each species. For each site, basin, stream, altitude and geographical coordinates are shown; also site location in MÚRRIA

Table S1. Haplotype composition (GenBank accession number) for each species. For each site, basin, stream, altitude and geographical coordinates are shown; also site location in M

et al. (2013) is given.

Species GenBank accession number Basin Stream Altitude Latitude Longitude Site

(MÚRRIA et al. 2013)

H. solerorum JQ687905, KF255657-KF255659 Guadalhorce Caballos 415 36º40'29"N 04º54'27"W B52

H. siltalai HM134823 Llobregat Llobregat 817 42º14'22"N 01º57'15"E H05

HM134823, HM134824 Llobregat Llobregat 760 42º14'11"N 01º53'46"E H06

HM134823, HM134824 Ter Ges 606 42º04'50"N 02º18'33"E H08

HM134829 Ter Sorreigs 550 42º00'11"N 02º11'46"E H09

HM134824 Llobregat Cardener 336 41º52'47"N 01º42'42"E H11

HM134823, HM134824 Tordera Arbúcies 335 41º49'04"N 02º29'49"E H12

HM134823, HM134824, HM134827, HM134828 Besós Vallcàrquera 376 41º43'41"N 02º16'58"E H13

HM134823, HM134825, HM134824 Besós Cànoves 420 41º42'34"N 02º20'55"E H14

JQ687902 Jara Arquillo 990 38º45'10"N 02º21'45"W T26

JQ687902 Segura Mundo 806 38º30'30"N 02º14'54"W T27

KF255651, KF255652 Genil Juntas 860 37º25'53"N 03º39'08"W B39

KF255651, KF255654 Genil Colomera 780 37º24'08"N 03º43'11"W B40

HM134823, KF255651, KF255652, KF255653 Guadiana Menor Gor 1333 37º20'53"N 02º56'55"W B41

KF255649, KF255650 Miel Miel 220 36º06'42"N 05º30'19"W B53

H. infernalis JQ687904 Genil Genil 1060 37º09'05"N 03º24'50"W B43

JQ687904 Guadiana Menor Tejos 1660 37º07'07"N 03º4'56"W B44

JQ687903 Genil Cerezal 1060 36º55'36"N 03º59'11"W B48

JQ687904 Genil Añales 970 36º55'02"N 03º53'32"W B49

H. fezana HM134821 Laou Laou 560 35º18'42"N 05º21'12"W R56

HM134821 Laou Laou 400 35º14'21"N 05º10'32"W R59

HM134821, HM134822 Laou Laou 905 35º06'48"N 05º11'08"W R61

H. fontinalis JQ687908 Guadiana Menor Guardal 1090 37º53'51"N 02º40'31"W T35

H. cf. spiritoi KF255655 Foix Pontons 540 41º25'12"N 01º30'30"E H16

KF255655 Foix Pontons 660 41º25'01"N 01º31'23"E H17

KF255655, KF255656, JQ687906 Ebre Canaletes 720 40º54'19"N 00º21'32"E H20

H. instabilis JQ687922 Llobregat Llobregat 817 42º14'22"N 01º57'15"E H05

KF255635, KF255637 Ter Ges 606 42º04'50"N 02º18'33"E H08

JQ687922 Tordera Arbúcies 335 41º49'04"N 02º29'49"E H12

KF255637 Tordera Arbúcies 335 41º49'04"N 02º29'49"E H12

JQ687922 Besós Cànoves 420 41º42'34"N 02º20'55"E H14

KF255634, KF255636 Ebre Canaletes 720 40º55'26"N 00º21'29"E H20

JQ687921 Guadalfeo Trevélez 1860 37º00'16"N 03º28'38"W B46

JQ687921 Genil Cerezal 1060 36º55'36"N 03º59'11"W B48

H. cf. spiritoi JQ687906 Llobregat Marganell 321 41º38'21"N 01º47'12"E

JQ687906 Leza/Ebro Tejada 882 42º12'33"N 02º30'17"W

H. spiritoi Hspi1, Hspi2 (KY678901, KY678902) Mandre Villadoro 660 37º42'43"N 14º17'51"E

Hspi3 (KY678903) Argens Bresque 376 43º34'57"N 05º08'60"E

Hspi3 (KY678903) Durance/Rhône Calavon 500 43º57'48"N 05º36'22"E Hspi3 (KY678903) Durance/Rhône Calavon 467 43º56'58"N 05º35'51"E Hspi3 (KY678903) Ouvèze/Rhône Toulourenc 582 44º10'30"N 05º25'60"E

H. ambigua HapC11 (KY656176) Deva Corvera 550 43º14'16"N 04º38'33"W HapC19, HapC42 (KY656184

,

HapC36 (KY656201) Sella Covadonga 137 43º18'53"N 05º04'18"W

HapC39 (KY656204) Cares Duje 855 43º13'36"N 04º45'22"W

H. tenuis HapC33 (KY656198) Ain Hérisson 785 46º36'33"N 05º53'01"E

Table S2. Complete locality information of individuals morphologically described. For each site, basin, stream and/or locality, altitude and geographical coordinates are shown.

Species Basin Stream/Locality Altitude Latitude Longitude

H. solerorum Guadalhorce Caballos/Tolox 415 36º40'31''N 04º54'27''O

Guadalhorce Caballos/Tolox 340 36º40'50''N 04º54'31''O

Guadalhorce Horcajos/Tolox 472 36º41'36''N 04º56'36''O

Guadalhorce Pereilas/Coín 206 36º39'16''N 04º47'15''O

Guadalhorce Corcho/Coín 428 36º36'37''N 04º47'33''O

H. cf. spiritoi Llobregat Coaner/Coaner 630 41º50'04''N 01º43'25''E

Foix Pontons/Pontons 660 41º25'04''N 01º31'22''E

Foix L’Albareda/Fontrubí 488 41º25'21''N 01º35'36''E

Llobregat Cornet/Sant Salvador de Guardiola 240 41º41'35''N 01º47'47''E

Llobregat Vilanova/Avinyó 470 41º54'30''N 01º58'26''E

Llobregat Sant Cugat/Sant Cugat del Racó 310 41º48'06''N 01º48'13''E

Llobregat Can Relat/Sentforas 567 41º54'25''N 02º12'35''E

Llobregat Gaià/Gaià 456 41º55'17''N 01º55'59''E

Llobregat Marganell/Marganell 321 41º38'23''N 01º47'12''E

Aragón/Ebro Bailo 701 42º30'35''N 00º48'46''O

Mijares Fuen Lozana/Mora de Rubielos 1037 40º15'10''N 00º45'17''O Jalón/Ebro Piedra/Monasterio de Piedra 741 41º11'40''N 01º47'13''O

Leza/Ebro Tejada/Jalón de Cameros 882 42º12'36''N 02º30'17''O

Segre Alinyà/Alinyà 931 42º10'48''N 01º25'24''E

H. spiritoi Durance/Rhône Calavon/Vailsaintes 500 43º57'51''N 05º36'23''E

Durance/Rhône Calavon/Simiane la Rotonde 467 43º56'59''N 05º35'51''E Ouvèze/Rhône Toulourenc/Montbrun les Bains 582 44º10'33''N 05º26'00''E