WintHUVHPHVWHU/22 QR Code / Link dieser Vorlesung:

WintHUVHPHVWHU/22 QR Code / Link dieser Vorlesung:

www.klichi.uni-muenster.de/folien

'UUHUQDW+DUWPXW6FKPLGW

&HQWUXPIU/DERUDWRULXPVPHGL]LQ

± =HQWUDOODERUDWRULXP±

8QLYHUVLWlWVNOLQLNXP0QVWHU

$OEHUW6FKZHLW]HU&DPSXV '0QVWHU 7HO )D[

]ODEOHKUHXQLPXHQVWHUGH +DUWPXW6FKPLGW=/#XNPXHQVWHUGH

Klinische Chemie und Laboratoriumsdiagnostik

Vorlesung: Molekulare Diagnostik

6RPPHUVHPHVWHU QR Code / Link dieser Vorlesung:

www.klichi.uni-muenster.de/folien

'UUHUQDW+DUWPXW6FKPLGW

&HQWUXPIU/DERUDWRULXPVPHGL]LQ

± =HQWUDOODERUDWRULXP±

8QLYHUVLWlWVNOLQLNXP0QVWHU

$OEHUW6FKZHLW]HU&DPSXV '0QVWHU 7HO )D[

]ODEOHKUHXQLPXHQVWHUGH +DUWPXW6FKPLGW=/#XNPXHQVWHUGH

Klinische Chemie und Laboratoriumsdiagnostik

Vorlesung: Molekulare Diagnostik

VWDWLVFKH*U|‰H G\QDPLVFKH*U|‰HQ

2PLFV$QVDW]GLHPROHNXODUH%LRORJLHGHU=HOOH

9DULDWLRQ

*HQH '1$(UEVXEVWDQ]

P51$

*HQRW\SLQJ

*HQRP *HQH3URILOLQJ

7UDQVNULSWRP ^3URWHRPLFV 3URWHRP 0HWDEROLF3URILOLQJ 0HWDERORP

*HQ$NWLYLWlW 3URWHLQYDULDQWH

0HWDEROLWH

$XINOlUXQJGHU'1$'RSSHOKHOL[6WUXNWXU

'LH5|QWJHQEHXJXQJVGLDJUDPPHGHU'1$GXUFK)UDQNOLQXQG :LONLQVXQGGHUHQPDWKHPDWLVFKH$QDO\VHWUXJHQZHVHQWOLFK ]XU$XINOlUXQJGHU'1$'RSSHOKHOL[VWUXNWXUEHLXQGEHVWlWLJWHQ GDVWKHRUHWLVFKH0RGHOOYRQ-DPHV:DWVRQXQG)UDQFLV&ULFN

1REHOSUHLV

9HQWHU&ROOLQV

&HOHUD'LDJQRVWLFV 'LHSRVWJHQRPLVFKH=HLW

'HUKXPDQHJHQHWLVFKH&RGHLVWHQGOLFKHQWVFKOVVHOW

hEHUGHU0LOOLDUGHQ%SVLQGQXQEHNDQQW

'LH5RKGDWHQDXVGHP+XPDQJHQRPSURMHNW VLQGIUHLYHUIJEDU

³7RGD\ZH>SOHGJH@WROHDGDJOREDOHIIRUWWR PDNHWKHUDZGDWDIURP'1$VHTXHQFLQJ DYDLODEOHWRVFLHQWLVWVHYHU\ZKHUHWREHQHILW SHRSOHHYHU\ZKHUH´

Ä3DUDGLVHORVW³QLFKWVRXQWHUVFKLHGOLFKZLH ZLUHVJHUQHKlWWHQ

Caenorhabitis elegans +RPRORJLH

GHV3URWHRPV

Drosophila melanogaster +RPRORJLH

GHV3URWHRPV

*OHLFKKHLW]XP6FKLPSDQVHQDXI '1$(EHQHHWZD

3UREHQPDWHULDOIUGLH0ROHNXODUGLDJQRVWLN

Geeignet

‡ 9ROOEOXW

‡ 3ODVPD

‡ 6HUXP

‡ buffy FRDW

‡ .QRFKHQPDUN

‡ 3XQNWDWHQ

‡ /\PSKR]\WHQ

‡ =HOONXOWXUHQ

‡ *HZHEHQ

‡ IRUHQVLVFKH3UREHQ

‡ &HUHEURVSLQDOIOVVLJNHLW

Nicht geeignet +HSDULQ%OXW Bedingt geeignet

*HWURFNQHWHV0DWHULDO

$XIO|VXQJLQ3%6

$XVEHXWH—O%OXW—J'1$

aQJ—O

'1$,VRODWLRQ6lXOHQV\VWHP '1$,VRODWLRQPDJQHWLFEHDGV9HUIDKUHQ

$XWRPDWLVLHUWH'1$,VROLHUXQJ

7HFDQ

0D[ZHOO

'DV3&5/DERU

'LH3RO\PHUDVH.HWWHQ5HDNWLRQ=XJSIHUG GHU0ROHNXODUELRORJLH

1REHOSUHLV IU&KHPLH

'1$

'RSSHOKHOL[ 'HQDWXULHUXQJ $QKHIWHQ

GHU3ULPHU .HWWHQ

YHUOlQJHUXQJ 9HUGRSSOXQJ GHU'1$

ƒ&

ƒ&

ƒ&

ƒ&

ƒ&

3&5=\NOXV

:LHGHUKROXQJHQ

3RO\PHUDVH.HWWHQ5HDNWLRQ3&5

0 QJ QJ SJ

ES

$JDURVH*HOHOHNWURSKRUHVH

5HDOWLPH3&5PLW

6<%5*UHHQ

ƒ&

ƒ&

ƒ&

ƒ&

ƒ&

3&5=\NOXV[

ƒ&

6FKPHO]NXUYHQ$QDO\VH

/LJKW&\FOHU0XWDWLRQVDQDO\VH 3&5XQG5HDOWLPH3&5 (LQH9LHO]DKOYRQ$QZHQGXQJHQ

,QIHNWLRQVGLDJQRVWLN9LUHQ3LO]H%DNWHULHQ

‡ 9LUXVGLDJQRVWLN+,9+%9+&9%.9(%9&09+699=9

‡ 6XEW\SHQ%HVWLPPXQJ± ]%,QIOXHQ]D$%Ä6FKZHLQHJULSSH³

‡ ,Q.XOWXUODQJVDPZDFKVHQGH(UUHJHU]%&KODP\GLHQ

%.9

.HLPHLQKDXVPO%OXW

9RUJHIHUWLJWH.LWV

3&5HLQH9LHO]DKOYRQ$QZHQGXQJHQ

*HQHWLVFKHU)LQJHUDEGUXFN

‡ %S ODQJHSRO\PRUSKH UHSHWLWLYHWDQGHPDUWLJH'1$6HTXHQ]HQ

‡ 6KRUWWDQGHP UHSHDWV 675 8QWHUVFKHLGXQJQDKHU9HUZDQGWH

‡ /HEHQVPLWWHOEHUZDFKXQJ ]%1DFKZHLVYRQ3IHUGHIOHLVFK

‡ 9DWHU RGHU0XWWHUVFKDIW

0 0XWWHU . .LQG ) 9DWHU

*HQHWLVFKH6W|UXQJHQGHV0HQVFKHQ

‡ +XPDQJHQHWLN(UENUDQNKHLWHQ

‡ 7XPRUJHQHWLN

‡ ,PPXQJHQHWLN+/$7\SLVLHUXQJ

‡ 3UlGLVSRVWLRQVGLDJQRVWLN7KURPERVH.+.5LVLNR

‡ 3KDUPDNRJHQHWLN

3&5XQG5HDOWLPH3&5 (LQH9LHO]DKOYRQ$QZHQGXQJHQ

$EVROXWH4XDQWLIL]LHUXQJ(UPLWWOXQJGHU*HQNRSLHQ]DKO

6RQGHQVSH]LILVFKH (FKW]HLW3&5PLW 6FKPHO]NXUYHQDQDO\VH

/LJKW&\FOHU 7DT0DQ

$%,3ULVP 4XDQWLWDWLYH (FKW]HLW3&5 µ1XFOHDVH$VVD\

'1$6HTXHQ]LHUXQJ

.DSLOODUVHTXHQ]LHUHU

$%,3ULVP

*HUlWHXQG0HWKRGHQLQGHU0ROHNXODUHQ'LDJQRVWLN WƌŝŶnjŝƉĚĞƌ^ĞƋƵĞŶnjŝĞƌƵŶŐŶĂĐŚĚĞŵ<ĞƚƚĞŶĂďďƌƵĐŚƉƌŝŶnjŝƉŶĂĐŚ^ĂŶŐĞƌ

'1$6HTXHQ]LHUXQJ

.DSLOODUHOHNWURSKRUHVH%LRLQIRUPDWLN+HXWH*ROGVWDQGDUG

¾ +RKH=XYHUOlVVLJNHLW

¾ /DQJH6HTXHQ]DEIROJHQOHVEDU

¾ $XWRPDWLVLHUEDU

)DPLOLlUHV0LWWHOPHHUILHEHUXQGDQGHUHYHUHUEOLFKHSHULRGLVFKH )LHEHUV\QGURPH

0ROHNXODUJHQHWLVFKH'LIIHUHQ]LDOGLDJQRVHVHLW0lU]LP=HQWUDOODERUHWDEOLHUW 'LH6\PSWRPHVLQG

+RKHVSHULRGLVFKDXIWUHWHQGHV)LHEHU(U\WKHPHXDDXFK%DXFK XQGRGHU7KRUD[VFKPHU]HQ 'LH7KHUDSLHLVWKlXILJDEKlQJLJYRQGHPEHWURIIHQHQ*HQE]ZGHU(UNUDQNXQJ 6SlWIROJHQ]%$P\ORLGRVH

&ƵũŝŬƵƌĂ͕<͗͘DŽůĞĐƵůĂƌ 'ĞŶĞƚŝĐƐ Θ'ĞŶŽŵŝĐ DĞĚŝĐŝŶĞ ϮϬϭϱ͖ϯ;ϰͿ͗ϮϳϮʹϮϴϮ

&ĂŵŝůŝćƌĞƐDŝƚƚĞůŵĞĞƌĨŝĞďĞƌ͕ŐĞŶĞƚŝƐĐŚĞsĂƌŝĂŶƚĞŶ;^EWƐͿƵŶĚDƵƚĂƚŝŽŶĞŶŝŵD&sͲ'ĞŶ

Dϲϵϰs ,ŽŵŽnjLJŐŽƚ Đ͘ϮϬϴϬх' ƌƐϲϭϳϱϮϳϭϳ ƉĂƚŚŽŐĞŶ

<ϲϵϱZ ,ŽŵŽnjLJŐŽƚ Đ͘ϮϬϴϰх' ƌƐϭϬϰϴϵϱϭϮϵ ƉĂƚŚŽŐĞŶ DƵƚĂƚŝŽŶƐŶĂĐŚǁĞŝƐŝŵD&sͲ'ĞŶŵŝƚƚĞůƐ^ĂŶŐĞƌ^ĞƋƵĞŶnjŝĞƌƵŶŐ

ƌďŐĂŶŐ͗ĂƵƚŽƐŽŵĂůƌĞnjĞƐƐŝǀ

([RQ

ůƚĞƌĞ^ĞƋƵĞŶnjŝĞƌĞƌǀĞƌƐĐŚŝĞĚĞŶĞƌ,ĞƌƐƚĞůůĞƌ

*HQRPVHTXHQ]LHUXQJPLWGHP

ϭ͘^ĐŚƌŝƚƚ

Ϯ͘^ĐŚƌŝƚƚ

ϯ͘^ĐŚƌŝƚƚ

ϰ͘^ĐŚƌŝƚƚ

0RQDWHIUGLH$QDO\VHGHV:DWVRQ*HQRPV 6WDUWGHV[KXPDQH*HQRPSURMHNW

7KH3ULPH0LQLVWHU8.KDVSOHGJHGWKDWWKH8.ZLOOPDSKXPDQ

JHQRPHVE\9HUDQVFKODJWH,QYHVWLWLRQVNRVWHQ…

1H[W1H[W*HQHUDWLRQ6HTXHQFLQJ6WDQG2NWREHU

*HQSDQHO ([RP ([RP*HQRP *HQRPÄ3RSXODWLRQVJHQRPH³

)ORZ&HOO 0L6HT

%ULGJHG3&51*6

1*6LQGHU5RXWLQH'LDJQRVWLNLP&I/VHLW0lU]P|JOLFK

%HVFKOXVVYRP0lU]

,P(LQKHLWOLFKHQ%HZHUWXQJVPD‰VWDE(%0GHU*HEKUHQRUGQXQJZLUG]XP-XOLGLH

$EUHFKHQEDUNHLWYRQ1*6HUP|JOLFKW

ŶǁĞŶĚƵŶŐĞŶĚĞƌE'^ͲDĞƚŚŽĚŝŬŝŶĚĞƌŵŽůĞŬƵůĂƌĞŶZŽƵƚŝŶĞĚŝĂŐŶŽƐƚŝŬ ϭͣ͘>ŽŶŐZĂŶŐĞWZ͞ďŝƐĐĂ͘ϴϬ<

ůůĞůƐƉĞnjŝĨŝƐĐŚĞ ,>dLJƉŝƐŝĞƌƵŶŐŵĞŚƌĞƌĞƌWĂƚŝĞŶƚĞŶŝŶĞŝŶĞƌŶĂůLJƐĞ;ŵĂdž͘ϯϴϰWĂƚŝĞŶƚĞŶͿ Ϯ͘ŶĂůLJƐĞǀŽŶsŝƌƵƐͲ ƵŶĚĂŬƚĞƌŝĞŶŐĞŶŽŵĞŶ͗

,LJŐŝĞŶĞEĂĐŚǁĞŝƐǀŽŶƌĞƐŝƐƚĞŶƚĞŶ<ƌĂŶŬĞŶŚĂƵƐŬĞŝŵĞŶĂƵƐǀŝĞůĞŶǀĞƌƐĐŚŝĞĚĞŶĞŶWƌŽďĞŶ ,/sͲ'ĞŶŽƚLJƉŝƐŝĞƌƵŶŐ ͣĞĞƉ ^ĞƋƵĞŶĐŝŶŐ͞ĨƌƺŚĞƌŬĞŶŶƵŶŐŶĞƵĞƌƌĞƐŝƐƚĞŶƚĞƌ^ƚćŵŵĞ ϯ͘ WĂŶĞůĚŝĂŐŶŽƐƚŝŬ

EĞƵƌŽŐĞŶĞƚŝŬ͗ϭϮWĂƚŝĞŶƚĞŶdžϮϬϬ'ĞŶĞƉƌŽZƵŶ;Dŝ^ĞƋͿ ,ƵŵĂŶŐĞŶĞƚŝŬ͗ϰϬ'ĞŶĞĚĞƐŚĞƌĞĚŝƚćƌĞŶDĂŵŵĂŬĂƌnjŝŶŽŵƐ DŽůĞŬƵůĂƌƉĂƚŚŽůŽŐŝĞ͗ͣĂŶĐĞƌWĂŶĞů͞ƚŚĞƌĂƉŝĞƌĞůĞǀĂŶƚĞ'ĞŶĞ ϰ͘džŽŵƐĞƋƵĞŶnjŝĞƌƵŶŐ

,ƵŵĂŶŐĞŶĞƚŝŬ͗DƵƚĂƚŝŽŶƐĂŶĂůLJƐĞďĞŝƵŶďĞŬĂŶŶƚĞƌsĞƌĚĂĐŚƚƐĚŝĂŐŶŽƐĞ ϱ͘'ĞŶŽŵƐĞƋƵĞŶnjŝĞƌƵŶŐ

,ƵŵĂŶŐĞŶĞƚŝŬ͗DƵƚĂƚŝŽŶƐĂŶĂůLJƐĞďĞŝƵŶďĞŬĂŶŶƚĞƌsĞƌĚĂĐŚƚƐĚŝĂŐŶŽƐĞ

džŽŵ͗ĐĂ͘ϭйĚĞƐ'ĞŶŽŵƐʹ ƵŵĨĂƐƐƚĂůůĞŬŽĚŝĞƌĞŶĚĞ'ĞŶĂďƐĐŚŶŝƚƚĞ

ĞĞƉƐĞƋƵĞŶĐŝŶŐ͗EĂĐŚǁĞŝƐǀŽŶDƵƚĂƚŝŽŶĞŶƵŶĚŐĞŶĞƚŝƐĐŚĞŶsĂƌŝĂŶƚĞŶŝŶǁĞŶŝŐĞŶĞůůĞŶŽĚĞƌĞůůƉŽƉƵůĂƚŝŽŶĞŶ

ƵƐǁĞƌƚƵŶŐƵŶĚĞĨƵŶĚĞƌƐƚĞůůƵŶŐŵŝƚ,ŝůĨĞǀŽŶ͗

Ͳ ĂƚĞŶďĂŶŬĞŶ;E/͕,'D͕/ŶĨĞǀĞƌƐͿ Ͳ sŽƌŚĞƌƐĂŐĞƉƌŽŐƌĂŵŵĞŶ;DƵƚĂƚŝŽŶdĂƐƚĞƌ͕WŽůLJƉŚĞŶ ϮͿ Ͳ ŝŶƐĐŚůćŐŝŐĞƌ&ĂĐŚůŝƚĞƌĂƚƵƌ

<ůĂƐƐŝĨŝŬĂƚŝŽŶĚĞƌŶĂĐŚŐĞǁŝĞƐĞŶĞŶ^ĞƋƵĞŶnjǀĂƌŝĂŶƚĞŶ

ŶĂĐŚZ/,Z^ĞƚĂů;ϮϬϭϱͿD'^ƚĂŶĚĂƌĚƐĂŶĚ 'ƵŝĚĞůŝŶĞƐ͕/ŶƚĞƌƉƌĞƚĂƚŝŽŶŽĨ ƐĞƋƵĞŶĐĞ ǀĂƌŝĂŶƚƐ

ϭ͘ EŝĐŚƚƉĂƚŚŽŐĞŶŽĚĞƌŬĞŝŶĞŬůŝŶŝƐĐŚĞ^ŝŐŶŝĨŝŬĂŶnj

^EWƐŽŚŶĞĨĨĞŬƚĂƵĨĚŝĞ&ƵŶŬƚŝŽŶĂůŝƚćƚĚĞƐWƌŽƚĞŝŶƐ

Ϯ͘ tĂŚƌƐĐŚĞŝŶůŝĐŚŶŝĐŚƚƉĂƚŚŽŐĞŶŽĚĞƌǀŽŶŐĞƌŝŶŐĞƌŬůŝŶŝƐĐŚĞƌ^ŝŐŶŝĨŝŬĂŶnj

^EWŽĚĞƌŚćƵĨŝŐĞDƵƚĂƚŝŽŶŵŝƚŵŝŶŽƐćƵƌĞĂƵƐƚĂƵƐĐŚŵŝƚĨƌĂŐůŝĐŚĞƌƵƐǁŝƌŬƵŶŐĂƵĨĚŝĞ&ƵŶŬƚŝŽŶĂůŝƚćƚ ϯ͘ DƵƚĂƚŝŽŶĞŶƵŶŬůĂƌĞƌŬůŝŶŝƐĐŚĞƌ^ŝŐŶŝĨŝŬĂŶnj

DƵƚĂƚŝŽŶĞŶŵŝƚŐĞƌŝŶŐĞƌƵƐǁŝƌŬƵŶŐĂƵĨĚŝĞ&ƵŶŬƚŝŽŶĂůŝƚćƚ͕ŚćƵĨŝŐͣ>ŽǁWĞŶĞƚƌĂŶĐĞ DƵƚĂƚŝŽŶĞŶ͞

ϰ͘ DƵƚĂƚŝŽŶƐŶĂĐŚǁĞŝƐŵŝƚŚŽŚĞƌŬůŝŶŝƐĐŚĞƌ^ŝŐŶŝĨŝŬĂŶnj

ǁĂŚƌƐĐŚĞŝŶůŝĐŚĨƺƌĚŝĞƌŬƌĂŶŬƵŶŐƵƌƐćĐŚůŝĐŚĞDƵƚĂƚŝŽŶͬĞŶŵŝƚsĞƌćŶĚĞƌƵŶŐĚĞƌ&ƵŶŬƚŝŽŶĂůŝƚćƚĚĞƐWƌŽƚĞŝŶƐ ϱ͘ DƵƚĂƚŝŽŶƐŶĂĐŚǁĞŝƐŵŝƚŬůĂƌĞƌŬůŝŶŝƐĐŚĞƌ^ŝŐŶŝĨŝŬĂŶnj

ƵƌƐćĐŚůŝĐŚĞDƵƚĂƚŝŽŶͬĞŶ͕ŵŝƚƐƚĂƌŬĞƌƵƐǁŝƌŬƵŶŐĂƵĨĚŝĞ&ƵŶŬƚŝŽŶŽĚĞƌ&ƵŶŬƚŝŽŶƐǀĞƌůƵƐƚĚĞƐWƌŽƚĞŝŶƐ Ͳ tŝůĚƚLJƉ ͗ŶĞŐĂƚŝǀ;<ĂƚĞŐŽƌŝĞϭǁŝƌĚŝŶĚĞƌZĞŐĞůnjƵŵtŝůĚƚLJƉ ŐĞƌĞĐŚŶĞƚͿ

Ͳ &ƌĂŐůŝĐŚ͗ <ĂƚĞŐŽƌŝĞϮƵŶĚϯ Ͳ WŽƐŝƚŝǀ͗ <ĂƚĞŐŽƌŝĞϰƵŶĚϱ

^ŝŶŐůĞEƵĐůĞŽƚŝĚĞWŽůLJŵŽƌƉŚŝƐŵ ;^EWͿ͗D&хϭй͕DƵƚĂƚŝŽŶ͗D&фϭй

$OOH0HQVFKHQVLQGHQJPLWHLQDQGHUYHUZDQGW

8QWHUVFKLHG]ZLVFKHQ GLHVHQEHLGHQ 3HUVRQHQYRQ

%DVHQ

'DVPHQVFKOLFKH*HQRP*%DVHQKDW FD0LOOLRQHQ(LQ]HOSROLPRUSKLVPHQ613V

FD0LOOLRQHQNXU]H,QVHUWLRQHQXQG'HOHWLRQHQVRZLHJU|‰HUH'HOHWLRQHQ FDGHUJHQHWLVFKHQ8QWHUVFKLHGH]ZLVFKHQ0HQVFKHQZHUGHQGXUFKFD613VLQ NRGLHUHQGHQ5HJLRQHQYHUXUVDFKW

:DVLVWHLQ*HQ&KLS"

ϮϬђŵ

Ύ Ύ Ύ Ύ

Ύ

*HQH&KLSŠ 3URFHVV)ORZ

:DVK 6WDLQ +\EULGL]H

KRXUV

)OXLGLFV6WDWLRQ

6FDQ $QDO\VLV

'DWD$QDO\VLV, DQG

4XDOLW\&RQWURO 'DWD$QDO\VLV,, +\EULGL]DWLRQ2YHQ

6FDQQHU

)LJWWHVWF RPSDULVRQV ZLWKFRQILGHQFHOLPLWV YDUHT 758(SDGMXVW%+GDWD.HPPLQJVLJQDOVFDOLQJ,45

*URXS7$77$7'(/'(/1(21(23030

*URXS6.6.6.

BDW BDW BDW BDW BDW BDW BDW BDW BDW BDW BDW BDW BDW BDW BDW BDW BDW BDW BDW BDW BDW BDW BDW BJBDW BDW BUBDW BIBDW BDW BDW BDW

3,*&KUSS/36LQGXFHG71)DOSKDIDFWRU /&3&KUTO\PSKRF \WHF\ WRVROLFSURWHLQ/SODV

$32'&KUTTWHUDSROLSRSURWHLQ'

$.5%&KUTDOGRNHWRUHGXFWDVHIDPLO\ PHPEHU 3.,$&KUTSURWHLQNLQDVHF$03GHSHQGHQWF DWDO 3).0&KUTSKRVSKRIUXFWRNLQDVHPXVFOH

&5,3&KUTF\VWHLQHULFKSURWHLQ

=1)&KUTTWHU]LQF ILQJHUSURWHLQ /*$/6&KUTTOHF WLQJDODFWRVLGHELQGLQJV ROXE

&36&KUTFDUEDPR\OSKRVSKDWHV\ QWKHWDVHPLWR /53&KUTORZGHQV LW\OLSRSURWHLQUHFHSWRUUHOD 571&KUTUHWLFXORQ 15*1&KUTQHXURJUDQLQSURWHLQNLQDVH&VXEVWUD (,)*&KUSHXNDU\RWLFWUDQV ODWLRQLQLWLDWLRQIDFWRU 73'&KUTWXPRUSURWHLQ' 63,17&KUTVHULQHSURWHDV HLQKLELWRU.XQLW]W\SH

$6$+/&KUT1DF\OVSKLQJRV LQHDPLGRK\ GURODVH .,$$&KUT.,$$SURWHLQ )%/1&KUSSILEXOLQ .,$$&KUS.,$$SURWHLQ +RPRVDSLHQVP51$F'1$'.)=S%IURPF 67$7&KUTVLJQDOWUDQVGXF HUDQGDF WLYDWRURIWUDQV 6&$5%&KUTVF DYHQJHUUHFHSWRUFODVV%PHP 0$*('&KU;SPHODQRPDDQWLJHQIDPLO\' 0$*('&KU;SPHODQRPDDQWLJHQIDPLO\' 0$*('&KU;SPHODQRPDDQWLJHQIDPLO\' 0$*('&KU;SPHODQRPDDQWLJHQIDPLO\' 0$*('&KU;SPHODQRPDDQWLJHQIDPLO\' :,5(&KUT:,5(SURWHLQ

$&$$&KUSSDFHW\ O&RHQ]\ PH$DF \OWUDQVIHUDV

SYDOXH [EDU\EDUGLIIUDWLR)SY DOXHGHVFULSWLRQFXWRIIWS GLII! )S UDWLR! SDJHRIQ RIDYYDOXHLQJURXSDYYDOXHLQJURXS

0XOWLVWDJH'HVLJQRIWKH:KROH*HQRPH

$VVRFLDWLRQ6WXG\

DQG6135HGXFWLRQ

&DVHV FRQWUROV

6WDJH , 6FUHHQLQJ

6WDJH ,, 5HSOLFDWLRQ

6WDJH ,,, 9HULILFDWLRQ

613V

IURP352&$0FRKRUW DWKLJKRULQWHUPHGLDWHULVN

DWORZULVN )ROORZXS0,VDW

6DPSOHV 0HQGHO

.

0HJ

7DT 0DQ

5LVN $OJRULWKP

7KHULVNFDOFXODWRU

9LVXDO%DVLF DSSOLFDWLRQ

&203$4 +DQGKHOG

$SSOLFDWLRQ

,QGLNDWLRQ *HQ $QDO\VH

+\SHUFKROHVWHULQlPLH $SR%$SR( 6RQGHQVSH]LILVFKH (FKW]HLW3&5 +\SHUFKROHVWHULQlPLH /'/53&6. 6HTXHQ]LHUXQJ

+lPRFKURPDWRVH +)( 6HTXHQ]LHUXQJ

0RUEXV:LOVRQ $73% 6HTXHQ]LHUXQJ

)DPLOLlUHDGHQRPDW|VH 3RO\SRVLV

$3& 6HTXHQ]LHUXQJ

6ROLGH 7XPRUH ]%S%5$).5$6 6HTXHQ]LHUXQJ1*6

)DPLOLlUHV0DPPD&DUFLQRP %5&$%5&$ 6HTXHQ]LHUXQJ1*6 0(1$0(1%IDPLOlUHV

6FKLOGGUVHQNDU]LQRP

5(7 6HTXHQ]LHUXQJ

3KDUPDNRJHQHWLN &\WRFKURPH 3

1$77307 6RQGHQVSH]LILVFKH(FKW]HLW 3&56HTXHQ]LHUXQJ

+,9,QIHNWLRQ +,951$ 6HTXHQ]LHUXQJ1*6

=ZLQJHQGQRWZHQGLJVHLWLQ.UDIWWUHWHQGHV

*HQGLDJQRVWLNJHVHW]HVDP