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Supplementary information (SI) 1

to the article entitled

Involvement of the auxin-cytokinin homeostasis in adventitious root formation of rose cuttings as affected by their nodal position in the stock plant

published in Planta

Authors:

Millicent A. Otiende, Klaus Fricke, Julius O.

Nyabundi, Kamau Ngamau, Mohammad R.

Hajirezaei, Uwe Druege

For correspondence:

Uwe Druege, Erfurt Research Centre for Horticultural Crops, University of Applied Sciences Erfurt, 99090 Erfurt, Germany

e-mail: uwe.druege@fh-erfurt.de

(2)

0 2000 4000 6000 8000 10000 12000 14000

-1 0 1 2 3 4 5 6 7 8 9

Top Bottom

NB, ∑CKs

StembaseBud region

Total cytokinins(pmolg-1freshmass)

RP, ∑CKs

DAP (a) (b)

Supplemental Figure S1 Concentration of total cytokinins (∑CKs in the bud region (a, b)and the stem base (c, d) of cuttings of theRosa hybridacultivars

‘Rosa progress‘ (RP, a, c) and ‘Natal briar‘ (NB, b, d) as affected by harvest position of cuttings within the stock plant (top, bottom) and days after planting (DAP). Mean values and SE (n= 4)

0 2000 4000 6000 8000

-1 0 1 2 3 4 5 6 7 8 9

0 1000 2000 3000 4000 5000 6000

-1 0 1 2 3 4 5 6 7 8 9

(c)

0 1000 2000 3000 4000 5000 6000 7000

-1 0 1 2 3 4 5 6 7 8 9

(d)

(3)

0.000 0.010 0.020 0.030 0.040

-1 0 1 2 3 4 5 6 7 8 9

0.00 0.01 0.01 0.02 0.02 0.03

-1 0 1 2 3 4 5 6 7 8 9

Top Bottom

NB, IPR / Z-type CKs

StembaseBud region

Ratio (IPR / Z-type CKs)

RP, IPR / Z-type CKs

DAP (a) (b)

Supplemental Figure S2: Ratio of IPR to Z-type cytokinins (CKs) in the bud region (a, b) and the stem base (c, d) of cuttings of the Rosa hybridacultivars

‘Rosa progress‘ (RP, a, c) and ‘Natal briar‘ (NB, b, d) as affected by harvest position of cuttings within the stock plant (top, bottom) and days after planting (DAP). Mean values and SE (n= 4)

(c) (d)

0.000 0.002 0.004 0.006 0.008 0.010 0.012 0.014

-1 0 1 2 3 4 5 6 7 8 9

0.000 0.003 0.006 0.009 0.012 0.015

-1 0 1 2 3 4 5 6 7 8 9

(4)

0.00 0.02 0.04 0.06 0.08 0.10 0.12 0.14 0.16

-1 0 1 2 3 4 5 6 7 8 9

0.00 0.01 0.02 0.03 0.04 0.05 0.06 0.07

-1 0 1 2 3 4 5 6 7 8 9

Top Bottom

NB, IPR / tZ-type CKs

StembaseBud region

Ratio (IPR / tZ-type CKs)

RP, IPR / tZ-type CKs

DAP (a) (b)

Supplemental Figure S3: Ratio of IPR to trans-Z-type cytokinins (CKs) in the bud region (a, b)and the stem base (c, d) of cuttings of theRosa hybrida cultivars‘Rosa progress‘ (RP, a, c) and ‘Natal briar‘ (NB, b, d) as affected by harvest position of cuttings within the stock plant (top, bottom) and days after planting (DAP). Mean values and SE (n= 4)

(c) (d)

0.00 0.01 0.02

-1 0 1 2 3 4 5 6 7 8 9

0.00 0.01 0.02 0.03 0.04

-1 0 1 2 3 4 5 6 7 8 9

(5)

0.00 0.01 0.02 0.03 0.04

-1 0 1 2 3 4 5 6 7 8 9

0.00 0.01 0.02 0.03 0.04 0.05

-1 0 1 2 3 4 5 6 7 8 9

0.00 0.02 0.04 0.06 0.08

-1 0 1 2 3 4 5 6 7 8 9

Top Bottom

NB, IAA / ∑CKs-type 2

StembaseBud region

Ratio (IAA / ∑Ck-type 2)

RP, IAA / ∑CKs-type 2

DAP (a) (b)

Supplemental Figure S4: Ratio of IAA to total cytokinins (∑CKs) of type 2 (excluding cis ZR) in the bud region (a, b) and the stem base (c, d) of cuttings of the Rosa hybridacultivars ‘Rosa progress‘ (RP, a, c) and ‘Natal briar‘ (NB, b, d) as affected by harvest position of cuttings within the stock plant (top, bottom) and days after planting (DAP). Mean values and SE (n= 4)

(c) (d)

0.00 0.01 0.02 0.03 0.04 0.05 0.06 0.07

-1 0 1 2 3 4 5 6 7 8 9

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