Supplementary information (SI) 1
to the article entitled
Involvement of the auxin-cytokinin homeostasis in adventitious root formation of rose cuttings as affected by their nodal position in the stock plant
published in Planta
Authors:
Millicent A. Otiende, Klaus Fricke, Julius O.
Nyabundi, Kamau Ngamau, Mohammad R.
Hajirezaei, Uwe Druege
For correspondence:
Uwe Druege, Erfurt Research Centre for Horticultural Crops, University of Applied Sciences Erfurt, 99090 Erfurt, Germany
e-mail: uwe.druege@fh-erfurt.de
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NB, ∑CKs
StembaseBud region
Total cytokinins(pmolg-1freshmass)
RP, ∑CKs
DAP (a) (b)
Supplemental Figure S1 Concentration of total cytokinins (∑CKs in the bud region (a, b)and the stem base (c, d) of cuttings of theRosa hybridacultivars
‘Rosa progress‘ (RP, a, c) and ‘Natal briar‘ (NB, b, d) as affected by harvest position of cuttings within the stock plant (top, bottom) and days after planting (DAP). Mean values and SE (n= 4)
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(c)
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(d)
0.000 0.010 0.020 0.030 0.040
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0.00 0.01 0.01 0.02 0.02 0.03
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NB, IPR / Z-type CKs
StembaseBud region
Ratio (IPR / Z-type CKs)
RP, IPR / Z-type CKs
DAP (a) (b)
Supplemental Figure S2: Ratio of IPR to Z-type cytokinins (CKs) in the bud region (a, b) and the stem base (c, d) of cuttings of the Rosa hybridacultivars
‘Rosa progress‘ (RP, a, c) and ‘Natal briar‘ (NB, b, d) as affected by harvest position of cuttings within the stock plant (top, bottom) and days after planting (DAP). Mean values and SE (n= 4)
(c) (d)
0.000 0.002 0.004 0.006 0.008 0.010 0.012 0.014
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0.000 0.003 0.006 0.009 0.012 0.015
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0.00 0.02 0.04 0.06 0.08 0.10 0.12 0.14 0.16
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0.00 0.01 0.02 0.03 0.04 0.05 0.06 0.07
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NB, IPR / tZ-type CKs
StembaseBud region
Ratio (IPR / tZ-type CKs)
RP, IPR / tZ-type CKs
DAP (a) (b)
Supplemental Figure S3: Ratio of IPR to trans-Z-type cytokinins (CKs) in the bud region (a, b)and the stem base (c, d) of cuttings of theRosa hybrida cultivars‘Rosa progress‘ (RP, a, c) and ‘Natal briar‘ (NB, b, d) as affected by harvest position of cuttings within the stock plant (top, bottom) and days after planting (DAP). Mean values and SE (n= 4)
(c) (d)
0.00 0.01 0.02
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0.00 0.01 0.02 0.03 0.04
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0.00 0.01 0.02 0.03 0.04
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0.00 0.01 0.02 0.03 0.04 0.05
-1 0 1 2 3 4 5 6 7 8 9
0.00 0.02 0.04 0.06 0.08
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NB, IAA / ∑CKs-type 2
StembaseBud region
Ratio (IAA / ∑Ck-type 2)
RP, IAA / ∑CKs-type 2
DAP (a) (b)
Supplemental Figure S4: Ratio of IAA to total cytokinins (∑CKs) of type 2 (excluding cis ZR) in the bud region (a, b) and the stem base (c, d) of cuttings of the Rosa hybridacultivars ‘Rosa progress‘ (RP, a, c) and ‘Natal briar‘ (NB, b, d) as affected by harvest position of cuttings within the stock plant (top, bottom) and days after planting (DAP). Mean values and SE (n= 4)
(c) (d)
0.00 0.01 0.02 0.03 0.04 0.05 0.06 0.07
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