Supplementary material
Table S1 - List of primers used for amplification and sequencing and PCR amplification conditions.
Marker Direction Primer Sequence PCR SEQ PCR conditions Primers reference COI Forward LCO1490-JJ 5'-
CHACWAAYCATAAAGATAT YGG-3'
X X
3min at 94°C, 36x (20s at 94°C,
45s at 50°C, 1min at 72°C),
2min at 72°C
Astrin and Stüben (2008)
COI Reverse HCO2198-JJ 5'-
AWACTTCVGGRTGVCCAA ARAATCA-3'
X X Astrin and Stüben (2008)
28S Forward Niph 15 5′-
CAAGTACCGTGAGGGAAA GTT-3′
X
3min at 94°C, 36x (20s at 94°C,
45s at 45°C, 1min at 72°C)
Verovnik et al. (2005)
28S Forward Niph 15i 5'-AGAGTCAAAAGA CCGTGAAACC-3'
X Weber et al. (2021)
28S Reverse Niph 16 5′-
AGGGAAACTTCGGAGGGA ACC-3′
X Verovnik et al. (2005)
28S Forward Niph 20 5′-
AAACACGGGCCAAGGAGT AT-3′
X Flot et al. (2010)
28S Reverse Niph 21 5′-
TATACTCCTTGGCCCGTGT T-3′
X Flot et al. (2010)
Table S2 – Species (alphabetical order) and GenBank accession numbers of the 28S sequences (GB 28S) used in the phylogenetic analysis. Outgroup genera and species used in the analysis are reported in bold.
Species name GB 28S Species name GB 28S
Carinurella paradoxa FS_16.003 OK030552 Niphargus lessiniensis EF617300 Chaetoniphargus lubuskensis MN914030 Niphargus liburnicus KT007477
Haploginglymus geos KY441086 Niphargus likanus KY617383
Haploginglymus morenoi KY441079 Niphargus lindbergi MN114033 Microniphargus leruthi KX379004 Niphargus longicaudatus EF617239 Niphargellus glenniei KC315617 Niphargus longidactylus EF617266 Niphargobates orophobata KR905879 Niphargus longiflagellum MN914004
Niphargopsis casparyi EU693291 Niphargus lourensis EU693312
Niphargus aberrans EF617260 Niphargus luchoffmani KX379014
Niphargus aggtelekiensis MN914031 Niphargus lunaris EU693313
Niphargus aitolosi EU693310 Niphargus malagorae KY617384
Niphargus alisadri KF581049 Niphargus maximus EF617279
Niphargus alpheus KY617132 Niphargus microcerberus MN114023
Niphargus altagahizi KF581059 Niphargus miljeticus KR905878
Niphargus ambulator KJ566699 Niphargus minor MN114028
Niphargus anchialinus KR905881 Niphargus mirocensis KR827047
Niphargus andropus KF218725 Niphargus molnari KP967555
Niphargus antipodes KY617097 Niphargus montanarius GU973419
Niphargus aquilex A1 KC315604 Niphargus montellianus KT878856 Niphargus aquilex A2 JF420874 Niphargus multipennatus KJ566700
Niphargus aquilex B KC315605 Niphargus muotae KX379024
Niphargus aquilex C KC315602 Niphargus murimali KX379026
Niphargus aquilex D KC315603 Niphargus novomestanus KY617364
Niphargus aquilex E KC315606 Niphargus ohridanus MN114029
Niphargus aquilex F KC315607 Niphargus orcinus EU693315
Niphargus arbiter KY617099 Niphargus pachypus KC733825
Niphargus arethusa EF617285 Niphargus pachytelson EU693316
Niphargus auerbachi EU693292 Niphargus parenzani MN913997
Niphargus aulicus MN914026 Niphargus pasquinii EF617244
Niphargus bajuvaricus EF617259 Niphargus patrizii MN914011
Niphargus balcanicus EF617280 Niphargus pectencoronatae MN914010 Niphargus bihorensis KF218726 Niphargus pectinicauda EF617258
Niphargus bilecanus JQ815550 Niphargus persicus KF581036
Niphargus bisitunicus KF581050 Niphargus pincinovae KY617139
Niphargus borisi KF581037 Niphargus plateaui GU973468
Niphargus boskovici KR827043 Niphargus podgoricensis KR905875 Niphargus brachytelson EU693293 Niphargus podpecanus KY617374
Niphargus brevicuspis MN914028 Niphargus poianoi KX379006
Niphargus brevirostris MN914008 Niphargus polymorphus EF617282
Niphargus brixianus EF617299 Niphargus pontoruffoi KF290027
Niphargus bureschi MN114020 Niphargus pretneri EF617294
Niphargus buturovici MN914022 Niphargus pupetta EU693318
Niphargus carniolicus EF617252 Niphargus puteanus EF617302 Niphargus carpathicus MN114019 Niphargus racovitzai KF290065
Niphargus cf. gallicus KF290033 Niphargus rejici EF617283
Niphargus cf. stefanellii MN914000 Niphargus rhenorhodanensis ABC EF025814 Niphargus cf. stenopus MN914009 Niphargus rhenorhodanensis DE EF025801 Niphargus cf. stygius 2 KX379016 Niphargus rhenorhodanensis DE EF025831 Niphargus cf. thienemanni MH172406 Niphargus rhenorhodanensis FG KX379033 Niphargus chagankae JQ815441 Niphargus rhenorhodanensis H KX379013 Niphargus cornicolanus MN914003 Niphargus rhenorhodanensis I EF025841 Niphargus costozzae EU693294 Niphargus rhenorhodanensis JK EF025810
Niphargus croaticus KT007482 Niphargus romuleus KX379010
Niphargus cvajcki KY617371 Niphargus salernianus MN914014
Niphargus dabarensis JQ815442 Niphargus salonitanus KY617147 Niphargus dalmatinus EF617296 Niphargus salzburgensis KJ566697
Niphargus dancaui KF290030 Niphargus sanctinaumi EU693320
Niphargus daniali KF581033 Niphargus schellenbergi 1 EF617267 Niphargus darvishi KF581041 Niphargus schellenbergi 2 KC315620
Niphargus decui KF290034 Niphargus scopicauda EF617261
Niphargus delamarei EU693295 Niphargus sharifi KF581048
Niphargus dimorphopus EU693296 Niphargus slovenicus EU693322 Niphargus dimorphus MN914035 Niphargus sohrevardensis KF581034
Niphargus dobati EF617247 Niphargus sp. Arkadi MN114032
Niphargus dobrogicus KF290076 Niphargus sp. BIH1 EF617268
Niphargus dolenianensis EF617269 Niphargus sp. HudaLuknja EF617246
Niphargus doli KY617137 Niphargus sp. Iran9 KF581040
Niphargus dolichopus EU693297 Niphargus sp. Iskavas EF617291
Niphargus elegans EF617297 Niphargus sp. ItalyA KJ566701
Niphargus enslini DW170805-029 OK030553 Niphargus sp. Jelovica MN114022 Niphargus enslini DW170827-001 OK030554 Niphargus sp. Croatia 1 MN914012
Niphargus factor EU693298 Niphargus sp. Croatia 2 MN914023
Niphargus fiseri MK911610 Niphargus sp. Meskla MN114031
Niphargus fjakae EF617290 Niphargus sp. Montenegro EF617278
Niphargus fongi MN914037 Niphargus sp. Neraidosplilios MN114030
Niphargus fontanus A1 KC315608 Niphargus sp. Podutik EF617251
Niphargus fontanus A2 EF617304 Niphargus sp. Prepadna EF617249
Niphargus fontanus C KC315609 Niphargus sp. Ro1 KF218728
Niphargus forelii A KC315615 Niphargus sp. Ro2 KF218724
Niphargus forelii B KC315616 Niphargus sp. Ro4 KF218732
Niphargus frasassianus GU973411 Niphargus sp. Ro5 KJ566693
Niphargus frontalis GU973423 Niphargus sp. Spain1 KY441088
Niphargus gammariformis MN114013 Niphargus sp. Spain2 KY441083
Niphargus gebhardti KP967556 Niphargus sp. Spain3 KY441081
Niphargus goricae KY617447 Niphargus sphagnicolus EF617270
Niphargus gottscheeanensis KY617394 Niphargus spinulifemur B EU693323
Niphargus grandii EU693300 Niphargus spoeckeri EU693324
Niphargus hadzii EU693301 Niphargus stefanellii MN913999
Niphargus hebereri MN913992 Niphargus stenopus EF617284
Niphargus hercegovinensis JQ815549 Niphargus steueri KT007489
Niphargus hosseiniei KF581055 Niphargus stochi JQ815551
Niphargus hrabei MN914029 Niphargus strouhali alpinus EF617254
Niphargus hvarensis EF617272 Niphargus stygius EU693325
Niphargus ictus GU973415 Niphargus styx KX379023
Niphargus ilamensis KF581039 Niphargus subtypicus EU693326
Niphargus illidzensis EU693304 Niphargus tatrensis EF617263
Niphargus irlandicus KC315618 Niphargus tauri EF617245
Niphargus iskae KY617382 Niphargus tauricus KF719274
Niphargus jovanovici MN114014 Niphargus thienemanni EF617301
Niphargus kapelanus KY617387 Niphargus thuringius KJ566695
Niphargus karamani EU693305 Niphargus timavi MN914034
Niphargus karkabounasi KP133156 Niphargus tonywhitteni KX379045
Niphargus kenki KY617370 Niphargus transsylvanicus KF218716
Niphargus khayyami JX535353 Niphargus tridentinus KT878857
Niphargus khwarizmi KF581056 Niphargus trullipes EF617281
Niphargus kochianus A KC315610 Niphargus urmiensis MK911608
Niphargus kochianus B KC315611 Niphargus vadimi KF719275
Niphargus kochianus C KC315612 Niphargus vinodolensis EF617298
Niphargus kolombatovici JQ815553 Niphargus virei A DQ119309
Niphargus kordunensis KY617386 Niphargus virei B KX379035
Niphargus koukourasi EF617277 Niphargus virei C EF617237
Niphargus krameri A EF617274 Niphargus vjetrenicensis EU693329
Niphargus krameri B MN914013 Niphargus wolfi A EF617250
Niphargus kusceri JQ815443 Niphargus zagorae KR827044
Niphargus labacensis EF617257 Niphargus zagrebensis EF617295
Niphargus ladmiraulti GU973463 Pseudoniphargus gorbeanus KY441101
Niphargus laisi EU693309 Pseudoniphargus portosancti KY441102
Niphargus laticaudatus KF218717 Pseudoniphargus sp. KY441100
Table S3 – Species, voucher codes, sites, geographical coordinates (WGS84), collectors and date of collection and GenBank accession numbers of the COI sequences used in the haplotype network.
Species name Voucher code Sampling site Admin. unit Country Latitude Longitude Collector Date COI GB Reference Niphargus enslini DW170827-001 Blätterteighöhle Baden-Württemberg Germany 47.8464 8.8590 B. Hugger, R. Grimm,
D. Weber 27/08/17 OK021662 This study Niphargus enslini DW170827-002 Blätterteighöhle Baden-Württemberg Germany 47.8464 8.8590 B. Hugger, R. Grimm,
D. Weber
27/08/17 OK021663 This study Niphargus enslini DW170827-003 Blätterteighöhle Baden-Württemberg Germany 47.8464 8.8590 B. Hugger, R. Grimm,
D. Weber
27/08/17 OK021664 This study Niphargus virei C Be_HT1 Water well in meadow in
Aubel
Liège Belgium 50.7119 5.8383 NA NA KC315707 McInerney et al. 2013
Niphargus virei C Be_HT2 Water well in Prés de l'Abreux
Liège Belgium 50.7294 5.6464 NA NA KC315708 McInerney et al. 2013
Niphargus virei B NC111 Grotte des Rutelins Val-de-Travers Switzerland 46.9107 6.5434 L. d'Hautefeuille 18/05/14 KX379087 Fišer et al. 2016 Niphargus virei B NC058 Grotte de Môttiers Val-de-Travers Switzerland 46.9051 6.6205 Vuilleumier, Farine 09/11/14 KX379098 Fišer et al. 2016 Niphargus virei B DW170430-071 Grotte des Faux
Monnayeurs Doubs France 47.0290 6.2881 J. Lips 30/04/17 OK021665 This study
Niphargus virei B DW170704-038 Tunnel de la Source Bleu Doubs France 46.8095 6.3241 D. Weber 04/07/17 OK021666 This study Niphargus virei C DW170318-020 Grotte de la
Cailleverdière
Saône-et-Loire France 46.4600 4.8000 L. Barriquand 18/03/17 OK021667 This study Niphargus virei C DW170713-020 Source du Château de
Fan
Gard France 44.1565 4.3587 V. Weber, D. Weber 13/07/17 OK021668 This study Niphargus virei C DW170719-001 Grotte de la Dragonière
de Labastide
Ardèche France 44.3555 4.4485 V. Weber, D. Weber 19/07/17 OK021669 This study
Table S4 – Morphometric data of the male of Niphargus enslini collected in the Blätterteighöhle (measures in mm).
Body length 19.60 Pereopod III Coxal plate length 1.82
Head leng 1.42 Coxal plate width 1.25
Antenna I Peduncle length 3.67 Basipodite length 2.60
Flagellum length 8.00 Ischiopodite length 0.39
Art. 1 accessory flagellum length 0.27 Meropodite length 1.60
Antenna II Peduncle length 3.47 Carpopodite length 1.09
Flagellum length 1.80 Propodite length 1.23
Mandibular palp Art. 1 length 0.35 Dactylopodite length 0.34
Art. 2 length 0.65 Dactylopodite claw length 0.27
Art. 3 length 0.76 Pereopod IV Coxal plate length 1.91
Gnathopod I Coxal plate width 1.54 Coxal plate width 1.91
Coxal plate heigth 1.12 Basipodite length 2.25
Basipodite length 1.83 Ischiopodite length 0.39
Basipodite width 0.70 Meropodite length 1.44
Carpopodite length 1.00 Carpopodite length 1.02
Propodite length 1.38 Propodite length 1.18
Propodite width 1.35 Dactylopodite length 0.32
Dactylopodite length 1.11 Dactylopodite claw length 0.25
Dactylopodite claw length 0.45 Pereopod V Basipodite length 2.33
Gnathopod II Coxal plate width 1.62 Basipodite width 1.60
Coxal plate heigth 1.23 Ischiopodite length 0.42
Basipodite length 2.12 Meropodite length 1.42
Basipodite width 0.65 Carpopodite length 1.68
Carpopodite length 1.05 Propodite length 2.00
Propodite length 1.64 Dactylopodite length 0.39
Propodite width 1.56 Dactylopodite claw length 0.27
Dactylopodite length 1.37 Pereopod VI Basipodite length 2.51
Dactylopodite claw length 0.52 Basipodite width 1.63
Uropod I Basipodite length 2.38 Ischiopodite length 0.59
Endopodite length 1.61 Meropodite length 1.85
Exopodite length 1.59 Carpopodite length 2.44
Uropod II Basipodite length 1.26 Propodite length 3.01
Endopodite length 1.10 Dactylopodite length 0.53
Exopodite length 0.90 Dactylopodite claw length 0.30
Uropod III Basipodite length 1.11 Pereopod VII Basipodite length 2.43
Endopodite length 0.60 Basipodite width 1.68
Exopodite art 1 length 4.57 Ischiopodite length 0.53
Exopodite art 2 length 2.90 Meropodite length 1.66
Telson Cleft length 0.75 Carpopodite length 2.20
Telson length 1.05 Propodite length 3.11
Telson width 0.80 Dactylopodite length 0.61
Longest spine length 0.30 Dactylopodite claw length 0.31
Figure S1 - Phylogenetic tree which allocate Niphargus enslini within the wider phylogeny of the genus Niphargus, reconstructed using Maximum Likelihood (IQTree2), based on 28S rRNA.
Ultrafast bootstrap supports are shown on nodes. The N. virei clade which includes N. enslini (100% support) is reported in red. Four Pseudoniphargidae were used as outgroups.
0.1 Niphargus_persicus_KF581036
Niphargus_rhenorhodanensis_DE_EF025831 Niphargus_pasquinii_EF617244
Niphargus_ladmiraulti_GU973463
Niphargus_fongi_MN914037
Niphargus_sp_Italy_A_KJ566701 Niphargus_likanus_KY617383
Niphargus_zagorae_KR827044 Niphargus_lindbergi_MN114033
Niphargus_sp_Prepadna_EF617249 Niphargus_krameri_B_MN914013
Niphargus_lunaris_EU693313
Niphargus_kochianus_A_KC315610
Niphargus_aberrans_EF617260 Niphargus_auerbachi_EU693292
Niphargus_forelii_B_KC315616 Niphargus_stefanellii_MN913999
Niphargus_sp_Montenegro_EF617278
Niphargus_luchoffmani_KX379014
Niphargus_boskovici_KR827043
Niphargus_virei_B_KX379035 Niphargus_tridentinus_KT878857
Niphargus_factor_EU693298
Niphargus_carpathicus_MN114019 Niphargus_aquilex_A1_KC315604
Niphargus_altagahizi_KF581059 Niphargus_thuringius_KJ566695
Niphargus_salonitanus_KY617147
Niphargus_karkabounasi_KP133156 Niphargus_khayyami_JX535353
Niphargus_schellenbergi_2_KC315620 Niphargus_rhenorhodanensis_H_KX379013
Niphargus_patrizii_MN914011 Niphargus_darvishi_KF581041
Niphargus_ambulator_KJ566699 Niphargus_trullipes_EF617281
Niphargus_pincinovae__KY617139 Niphargus_sohrevardensis_KF581034
Niphargus_rhenorhodanensis_DE_EF025801
Niphargus_sp_Spain2_KY441083
Niphargus_dobati_EF617247 Niphargus_longiflagellum_MN914004
Niphargus_hrabei_MN914029
Niphargus_salzburgensis_KJ566697
Niphargus_cf_gallicus_KF290033
Niphargus_sp_Ro2_KF218724 Niphargus_sanctinaumi_EU693320
Niphargus_jovanovici_MN114014
Niphargus_sp_Meskla_MN114031 Niphargus_timavi_MN914034
Niphargus_spinulifemur_B_EU693323
Niphargus_murimali_KX379026 Niphargus_zagrebensis_EF617295
Niphargus_enslini_DW170805-029
Niphargus_virei_A_DQ119309 Niphargus_orcinus_EU693315
Niphargus_schellenbergi_1_EF617267 Niphargus_sphagnicolus_EF617270
Microniphargus_leruthi_KX379004
Niphargus_kochianus_B_KC315611
Niphargus_sp_Arkadi_MN114032 Niphargus_thienemanni_EF617301
Niphargus_multipennatus_KJ566700 Niphargus_costozzae_EU693294
Niphargus_sp_Jelovica_MN114022 Niphargus_antipodes__KY617097
Niphargus_tauri_EF617245 Niphargus_miljeticus_KR905878
Niphargus_mirocensis_KR827047
Carinurella_paradoxa_FS_16.003 Niphargus_kenki_KY617370
Niphargus_dabarensis_JQ815442 Niphargus_sp_BIH1_EF617268
Niphargus_hosseiniei_KF581055
Niphargus_arbiter_KY617099
Niphargus_delamarei_EU693295
Pseudoniphargus_gorbeanus_KY441101 Niphargus_kordunensis_KY617386
Niphargus_elegans_EF617297
Niphargus_urmiensis_MK911608
Niphargus_dimorphopus_EU693296 Niphargus_vinodolensis_EF617298
Niphargus_hvarensis_EF617272
Niphargus_decui_KF290034 Niphargus_aulicus_MN914026
Niphargus_ilamensis_KF581039 Niphargus_montellianus_KT878856
Niphargus_sp_Ro5_KJ566693
Niphargus_hebereri_MN913992
Niphargus_sp_Spain1_KY441088 Niphargus_frasassianus_GU973411
Niphargus_enslini_DW170827-001 Chaetoniphargus_lubuskensis_MN914030
Niphargopsis_casparyi_EU693291
Niphargus_kusceri_JQ815443
Niphargus_aggtelekiensis_MN914031 Niphargus_rhenorhodanensis_ABC_EF025814
Niphargus_salernianus_MN914014 Niphargus_laticaudatus_KF218717
Niphargus_gammariformis_MN114013 Niphargus_borisi_KF581037
Niphargus_hercegovinensis_JQ815549 Niphargus_illidzensis_EU693304
Pseudoniphargus_sp_KY441100 Niphargus_sp_Ro1_KF218728
Niphargus_cf_stefanellii_MN914000
Niphargus_grandii_EU693300 Niphargus_poianoi_KX379006
Niphargus_stenopus_EF617284
Niphargus_brevicuspis_MN914028
Niphargus_sp_Ro4_KF218732 Niphargus_scopicauda_EF617261
Niphargus_maximus_EF617279
Niphargus_stygius_EU693325 Niphargus_sp_Iran_9_KF581040
Niphargus_croaticus_KT007482 Niphargus_sp_Iskavas_EF617291
Niphargus_brixianus_EF617299
Niphargellus_glenniei_KC315617
Niphargus_rhenorhodanensis_JK_EF025810
Haploginglymus_geos_KY441086 Niphargus_aitolosi_EU693310
Niphargus_podpecanus_KY617374
Niphargus_fontanus_A1_KC315608
Niphargus_labacensis_EF617257 Niphargus_laisi_EU693309
Niphargus_gebhardti_KP967556
Niphargus_bihorensis_KF218726 Niphargus_vjetrenicensis_EU693329
Niphargus_gottscheeanensis_KY617394
Niphargus_sp_Spain3_KY441081
Niphargus_irlandicus_KC315618
Niphargus_wolfi_A_EF617250 Niphargus_parenzani_MN913997
Niphargus_dobrogicus_KF290076 Niphargus_lourensis_EU693312
Niphargus_longicaudatus_EF617239
Niphargus_pectencoronatae_MN914010
Haploginglymus_morenoi_KY441079 Niphargus_tauricus_KF719274
Niphargus_ohridanus_MN114029
Niphargus_fontanus_A2_EF617304 Niphargus_lessiniensis_EF617300
Niphargus_muotae_KX379024
Niphargus_karamani_EU693305 Niphargus_chagankae_JQ815441
Niphargus_kolombatovici_JQ815553 Niphargus_brachytelson_EU693293
Niphargus_buturovici_MN914022
Niphargus_pachypus_KC733825
Niphargus_andropus_KF218725 Niphargus_aquilex_B_KC315605 Niphargus_sharifi_KF581048
Niphargus_cornicolanus_MN914003 Niphargus_plateaui_GU973468 Niphargus_iskae_KY617382
Niphargus_rhenorhodanensis_FG_KX379033 Niphargus_dalmatinus_EF617296
Niphargus_puteanus_EF617302 Niphargus_malagorae_KY617384
Niphargus_bisitunicus_KF581050
Niphargus_rejici_EF617283
Niphargus_polymorphus_EF617282 Niphargus_sp_MN914023
Niphargus_microcerberus_MN114023 Niphargus_sp_Neraidosplilios_MN114030
Niphargus_pupetta_EU693318 Niphargus_alpheus__KY617132
Niphargus_vadimi_KF719275 Niphargus_cvajcki_KY617371
Niphargobates_orophobata_KR905879
Niphargus_virei_C_EF617237 Niphargus_alisadri_KF581049
Niphargus_koukourasi_EF617277
Niphargus_cf_thienemanni_MH172406
Niphargus_krameri_A_EF617274
Niphargus_bajuvaricus_EF617259 Niphargus_montanarius_GU973419
Niphargus_dancaui_KF290030
Niphargus_aquilex_A2_JF420874
Niphargus_dimorphus_MN914035 Niphargus_stochi_JQ815551
Niphargus_tatrensis_EF617263 Niphargus_cf_stygius_2_KX379016
Niphargus_aquilex_D_KC315603 Niphargus_bureschi_MN114020
Niphargus_sp_Podutik_EF617251 Niphargus_arethusa_EF617285
Niphargus_slovenicus_EU693322
Niphargus_styx_KX379023
Niphargus_fontanus_C_KC315609 Niphargus_pachytelson_EU693316
Niphargus_podgoricensis_KR905875 Niphargus_dolenianensis_EF617269
Niphargus_daniali_KF581033 Niphargus_sp_HudaLuknja_EF617246
Niphargus_carniolicus_EF617252 Niphargus_fiseri_MK911610
Niphargus_bilecanus_JQ815550 Niphargus_hadzii_EU693301
Niphargus_aquilex_C_KC315602
Niphargus_pontoruffoi_KF290027 Niphargus_sp_MN914012
Niphargus_frontalis_GU973423
Niphargus_racovitzai_KF290065 Niphargus_spoeckeri_EU693324
Niphargus_aquilex_E_KC315606 Niphargus_longidactylus_EF617266 Niphargus_novomestanus_KY617364
Niphargus_ictus_GU973415
Niphargus_minor_MN114028 Niphargus_goricae_KY617447
Niphargus_brevirostris_MN914008
Niphargus_anchialinus_KR905881
Niphargus_balcanicus_EF617280 Niphargus_tonywhitteni_KX379045
Niphargus_khwarizmi_KF581056 Niphargus_pretneri_EF617294
Pseudoniphargus_portosancti_KY441102 Niphargus_forelii_A_KC315615
Niphargus_dolichopus_EU693297
Niphargus_transsylvanicus_KF218716 Niphargus_cf_stenopus_MN914009
Niphargus_liburnicus_KT007477
Niphargus_rhenorhodanensis_I_EF025841 Niphargus_kapelanus_KY617387
Niphargus_strouhali_alpinus_EF617254 Niphargus_molnari_KP967555 Niphargus_fjakae_EF617290
Niphargus_aquilex_F_KC315607 Niphargus_doli_KY617137
Niphargus_kochianus_C_KC315612 Niphargus_subtypicus_EU693326
Niphargus_steueri_KT007489
Niphargus_pectinicauda_EF617258 Niphargus_romuleus_KX379010
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