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Supplementary Table 1 Primer sequences for this manuscript

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Genes Forward primer sequence Reverse primer sequence

GAPDH GGAGCGAGATCCCTCCAAAAT GGCTGTTGTCATACTTCTCATGG OCT4 CCTCACTTCACTGCACTGTA CAGGTTTTCTTTCCCTAGCT NANOG TGAACCTCAGCTACAAACAG TGGTGGTAGGAAGAGTAAAG SOX2 CCCAGCAGACTTCACATGT CCTCCCATTTCCCTCGTTTT SOX1 AATTTTATTTTCGGCGTTGC TGGGCTCTGTCTCTTAAATTTGT PAX6 ATGTGTGAGTAAAATTCTGGGCA GCTTACAACTTCTGGAGTCGCTA FOXG1 GAGCGACGACGTGTTCATC GCCGTTGTAACTCAAAGTGCTG CDH1 GCTGGACCGAGAGAGTTTCC CGACGTTAGCCTCGTTCTCA ZIC1 ACATGAAGGTCCACGAATCCT CTTGTGGTCGGGTTGTCTGT PAX3 AGCTCGGCGGTGTTTTTATCA CTGCACAGGATCTTGGAGACG ERBB3 GGTGATGGGGAACCTTGAGAT CTGTCACTTCTCGAATCCACTG TFAP2A GACTCGGAGACCTCTCGATCC GACGGCATTGCTGTTGGAC FOXD3 AGCCTAGTGAAGCCGCCTTACT TTGTCGAACATGTCCTCGGACT BMP4 CGTCCAAGCTATCTCGAGCC CGGAATGGCTCCATAGGTCC KDR GAGGGGAACTGAAGACAGGC GGCCAAGAGGCTTACCTAGC CXCL12 GCCCTTCAGATTGTAGCCCG GTAAGGGTTCCTCAGGCGTC FOXH1 CCCCCAGAGGCAGAGTCG GACCTGACGGATGATCTGGG KRT8 CTCAAAGGCCAGAGGGCTTC ACTTGGCGTTGGCATCCTTA KRT18 GAGGGCTCAGATCTTCGCAA CCAGCTGCAGTCGTGTGATA KRT19 GTTCACCAGCCGGACTGAA GCAGGTCAGTAACCTCGGAC VEGFA TCACCATGCAGATTATGCGGA TACCGGGATTTCTTGCGCTT CLDN6 TTATCTCCTTCGCAGTGCAGCTC CACGATGCTGTTGCCGATGA OTX2 CAAAGTGAGACCTGCCAAAAAGA TGGACAAGGGATCTGACAGTG EZH2 GCAGGCTGGGGGATTTTTATCA AACGAATTTTGTTACCCTTGCGG DLX5 CAGCCAAAGCTTATGCCGAC CGGTCACTTCTTTCTCTGGCT SOX2-shRNA1# CAGCTCGCAGACCTACATGAA

SOX2-shRNA2# CGCTCATGAAGAAGGATAAGT SOX2-shRNA3# GTACAGTATTTATCGAGATAA SOX2-shRNA4# AGGAGCACCCGGATTATAAAT

Supplementary Table 1 Primer sequences for this manuscript

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Name Company Cat. # Host Dilution

SOX2 R&D mab2018 Mouse 1:1000

PAX6 Biolegend PRB-278P Rabbit 1:1000

FLAG Sigma F1804 Mouse 1:1000

HRP-GAPDH Proteintech HRP-60004 null 1:10000

PAX6-488 BD 561664 Mouse 1:100

NESTIN Millipore ABD69 Rabbit 1:1000

MAP2 CST 8707 Rabbit 1:1000

GFAP Millipore MAB360 Mouse 1:1000

OCT4 SantaCruz SC-5279 Mouse 1:500

TUBB3 GeneTex GTX130245 Rabbit 1:1000

EZH2 CST 5246S Rabbit 1:100

H3K27me3 Diagenode C15410069 Rabbit 1:100

SOX2 R&D AF2018 Goat 1:100

Supplementary Table 2 Antibody information for this manuscript

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Supplementary Table 1 | Primers used in this study.NameForward primer (5'-3')Reverse primer (5'-3')EnzymeCategoryVectorM1TCGGAGTCCAAACAAAAATGCATGCGAAGTCATTGGTCACFine mappingM2ATTGGTTTTGAGCCTTTGATAATAGATACGAAAAAATACTTTACGCACTTNsi

MS1-CDS ttctACGCGTATGACGGGAACACCTGTGG ggctTCTAGACAAATTTAGAAGATTTGATAT Subcellular localization in Nicotiana benthamiana PTF101-GFP

Supplementary Table 1. Primer sequences used in this paper. Conventional RT-PCR Gene name

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