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Table S1 Clinical and pulmonary function characteristics of all subjects

Non-smokers (healthy) Smokers with COPD

Subject (n) 10 10

Age (years) 60.8 ± 4.2 64.5 ± 3.8

Sex (male/female) 7/3 9/1

Smoking history (pack-years) 0 61.5 ± 5.2

Smoking years 0 46.6  ±  2.8*

FEV1 (% predicted) 86.5 ±  4.4 32.8 ±  3.9*

FEV1/FVC (%) 81.7  ±  6.2 47.1  ±  6.6*

GOLD stage

1 - 1

2 - 7

3 - 2

4 - 0

Note: * p < 0.05 vs. non-smokers. COPD, chronic obstructive pulmonary disease.

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Table S2 Silent sequence

Primers Target sequence (5’-3’)

sh-NC CCTAAGGTTAAGTCGCCCTCG

sh-p38 MAPK-1 GGACCTCCTTATAGACGAATG

sh-p38 MAPK-2 GGCACACTGATGACGAAATGA

Note: NC, negative control; p38 MAPK, p38 mitogen-activated protein kinase.

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Table S3 Primer sequences for RT-qPCR

Genes Primer sequences (5’-3’)

VWF F: GACTTCAAAGCCCCTGGACA

R: CCATACAAACAGGGGCCGTA

p38 MAPK F: ATAATGCGTCTGACGGGGAC

R: GGGTCGTGGTACTGAGCAAA

GAPDH F: AGTGCCAGCCTCGTCTCATA

R: ACCAGCTTCCCATTCTCAGC

Note: RT-qPCR, reverse transcription quantitative polymerase chain reaction; VWF, von Willebrand factor; p38 MAPK, p38 mitogen-activated protein kinase; GAPDH, glyceraldehyde-3- phosphate dehydrogenase.

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