Supplementary Material for: Hyland et al. Stable Carbon and Nitrogen Isotope Variability of Bone Collagen to Determine the Number of Isotopically Distinct Specimens

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Supplementary Material for: Hyland et al. Stable Carbon and Nitrogen Isotope Variability of Bone Collagen to Determine the Number of Isotopically Distinct Specimens

Table S5: Isotopic results and indicators of contamination for additional samples analysed in testing the utility of the NIDS metric.

Sampl

e # Animal Animal

# Bone Bone# wt

%C wt

%N C:N δ¹³C δ¹⁵N

6842 Cow 8 Talus 1 45.3 16.6 3.18 17.04 +7.11

6842 Cow 8 Talus 1 45.3 16.7 3.17 17.14 +7.06

6843 Cow 8 Calcaneus 2 45.5 16.6 3.19 16.53 +7.05

6844 Cow 8 Calcaneus epiphysis 3 45.2 16.6 3.18 15.22 +6.86

6845 Cow 8 Navicular 4 45.7 16.7 3.18 14.46 +6.90

6846 Cow 8 talus-2 - TI, TII, & TIII 5 44.8 16.4 3.19 17.25 +7.00

6847 Cow 8 distal tibia 6 44.9 16.5 3.16 14.37 +6.87

6847 Cow 8 distal tibia 6 44.1 16.3 3.16 14.33 +6.91

6848 Cow 8 talus-1 - Sustentaculum tali 7 44.5 16.4 3.18 15.76 +6.91

6850 Cow 9 Calcaneus 2 44.1 16.2 3.18 19.35 +6.18

6851 Cow 9 Navicular 3 42.2 15.4 3.20 20.38 +6.33

6852 Cow 9 distal tibia 4 43.0 15.7 3.19 20.07 +6.32

6854 Cow 10 Talus 1 42.8 15.7 3.17 20.63 +5.92

6855 Cow 10 Calcaneus 2 46.3 17.0 3.17 20.21 +5.89

6856 Cow 10 Navicular 3 44.6 16.5 3.15 20.36 +6.00

6857 Cow 10 distal tibia 4 45.0 16.6 3.16 20.16 +5.94

6859 Cow 11 Rib 1 42.6 15.5 3.21 9.89 +6.58

6859 Cow 11 Rib 1 40.9 14.9 3.21 9.87 +6.65

6860 Cow 11 Rib 2 42.9 15.4 3.24 10.70 +6.74

6861 Cow 11 Rib 3 41.7 15.0 3.24 9.73 +6.59

6862 Cow 11 Rib 4 41.0 15.0 3.19 19.37 +7.15

6863 Chicken 1 Fibula 1 45.2 16.2 3.25 17.50 +3.90

6864 Chicken 1 Scapula 2 42.4 15.0 3.29 17.54 +3.93

6865 Chicken 1 Scapula 3 46.7 16.9 3.22 17.21 +3.80

6865 Chicken 1 Scapula 3 46.8 16.9 3.22 17.21 +3.88

6866 Chicken 1 Radius 4 51.1 18.1 3.29 17.48 +3.97

6868 Chicken 1 Ulna 6 40.8 14.4 3.30 17.47 +3.86

6868 Chicken 1 Ulna 6 41.2 14.5 3.31 17.44 +3.82

6869 Chicken 1 Ulna 7 43.8 15.8 3.23 17.00 +3.75

6870 Chicken 1 Clavicle 8 45.2 15.9 3.32 17.36 +3.90

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Sampl

e # Animal Animal

# Bone Bone# wt

%C wt

%N C:N δ¹³C δ¹⁵N

6871 Chicken 1 Covacoid 9 44.9 16.3 3.21 16.86 +3.77

6873 Chicken 1 Femur 11 44.2 15.8 3.27 16.99 +3.76

6873 Chicken 1 Femur 11 45.1 16.0 3.28 16.98 +3.77

6874 Chicken 1 Femur 12 45.3 16.1 3.29 16.89 +3.83

6875 Chicken 1 Humerus 13 45.5 16.4 3.23 16.59 +3.78

6876 Chicken 1 Humerus 14 42.0 14.8 3.32 17.14 +3.92

6877 Chicken 1 Tibia 15 44.9 15.9 3.29 17.07 +3.84

6882 Chicken 2 Fibula 4 40.9 14.5 3.30 16.87 +3.48

7449 Chicken 2 Scapula 7 45.5 16.1 3.30 16.42 +3.09

7450 Chicken 2 Tibia 8 45.5 16.1 3.30 16.09 +2.89

7451 Chicken 2 Tibia 9 45.5 16.1 3.29 16.16 +2.91

7452 Chicken 2 Femur 10 44.5 15.9 3.26 16.02 +3.08

7453 Chicken 2 Femur 11 44.6 16.0 3.25 15.98 +2.89

7453 Chicken 2 Femur 11 45.6 16.3 3.26 16.05 +3.00

7454 Chicken 2 Humerus 12 44.4 15.9 3.27 15.97 +2.83

7455 Chicken 2 Humerus 13 42.7 15.2 3.28 16.28 +3.03

7456 Chicken 2 covacoid 14 27.4 9.7 3.30 16.82 +3.25

7458 Chicken 2 Ulna 16 42.1 15.0 3.28 16.41 +3.12

7459 Chicken 2 Ulna 17 44.9 16.0 3.28 16.16 +2.91

7460 Chicken 2 Radius 18 44.4 15.8 3.27 16.33 +2.95

7464 Chicken 2 Os coxae 22 44.6 15.7 3.31 16.56 +3.20

7468 Chicken 2 Tarsometatarsus 26 44.7 15.7 3.31 16.37 +2.85

7474 Chicken 3 Humerus 6 45.0 16.0 3.27 16.93 +3.18

7475 Chicken 3 Humerus 7 45.2 16.2 3.25 16.61 +3.10

7476 Chicken 3 Radius 8 45.1 16.2 3.24 17.05 +3.23

7476 Chicken 3 Radius 8 45.1 16.2 3.25 17.05 +3.16

7477 Chicken 3 Radius 9 44.8 16.1 3.24 17.24 +3.31

7478 Chicken 3 Ulna 10 45.5 16.1 3.30 17.15 +3.16

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Sampl

e # Animal Animal

# Bone Bone# wt

%C wt

%N C:N δ¹³C δ¹⁵N

7479 Chicken 3 Ulna 11 45.8 16.1 3.32 17.15 +3.20

7480 Chicken 3 Tibia 12 45.9 16.4 3.26 17.31 +3.12

7481 Chicken 3 Tibia 13 45.1 16.3 3.23 17.19 +3.26

7482 Chicken 3 Femur 14 45.0 16.3 3.22 16.79 +3.23

7482 Chicken 3 Femur 14 44.9 16.3 3.22 16.79 +3.24

7483 Chicken 3 Femur 15 45.3 16.3 3.24 16.72 +3.30

7484 Cow 12 Rib 1 44.8 16.3 3.20 10.81 +6.63

7485 Cow 12 Rib 2 43.5 16.1 3.15 16.25 +7.55

7486 Cow 12 Rib 3 41.3 15.1 3.18 12.11 +6.88

7487 Cow 12 Rib 4 44.7 16.3 3.20 13.60 +6.78

7488 Chicken 4 Tibia 1 45.2 16.4 3.22 14.76 +3.56

7488 Chicken 4 Tibia 1 45.1 16.4 3.21 14.77 +3.61

7489 Chicken 4 Tibia 2 45.8 16.1 3.31 15.52 +3.78

7490 Chicken 4 Fibula 3 43.5 15.4 3.29 15.18 +3.91

7491 Chicken 4 Scapula 4 44.8 16.4 3.18 14.59 +3.68

7492 Chicken 4 Femur 5 44.8 15.9 3.28 14.98 +3.76

7493 Chicken 4 Femur 6 44.9 15.8 3.31 15.24 +3.86

7494 Chicken 4 Coracoid 7 45.1 16.0 3.28 15.21 +3.73

7495 Chicken 4 Radius 8 46.1 16.7 3.21 14.92 +3.57

7495 Chicken 4 Radius 8 45.7 16.6 3.22 14.89 +3.48

7496 Chicken 4 Radius 9 46.4 16.4 3.29 15.23 +3.58

7595 Chicken 4 Humerus 10 46.1 16.4 3.29 14.65 +3.39

7596 Chicken 4 Humerus 11 46.1 16.4 3.27 14.84 +3.44

7597 Chicken 4 Ulna 12 46.0 16.4 3.28 14.99 +3.66

7598 Chicken 4 Ulna 13 45.2 16.0 3.29 14.93 +3.59

7598 Chicken 4 Ulna 13 45.6 16.0 3.31 14.98 +3.67

7600 Chicken 4 Sternum (keel) 15 46.0 16.3 3.28 17.15 +3.58

7601 Chicken 5 Ulna 1 45.9 16.3 3.29 17.09 +3.19

7603 Chicken 5 Femur 3 46.7 16.5 3.31 17.24 +3.28

7603 Chicken 5 Femur 3 46.1 16.2 3.31 17.20 +3.17

Sampl Animal Animal Bone Bone# wt wt C:N δ¹³C δ¹⁵N

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e # # %C %N

7604 Chicken 5 Femur 4 45.5 16.3 3.26 16.85 +3.23

7605 Chicken 5 Scapula 5 46.2 16.5 3.27 17.12 +3.29

7607 Chicken 5 Radius 7 42.2 14.8 3.32 17.42 +3.29

7608 Chicken 5 Radius 8 42.4 15.1 3.27 17.27 +3.30

7609 Chicken 5 Humerus 9 43.7 15.3 3.32 16.89 +3.08

7610 Chicken 5 Humerus 10 43.8 15.6 3.27 16.92 +3.05

7610 Chicken 5 Humerus 10 43.8 15.6 3.28 16.97 +3.06

7611 Chicken 5 Tibia 11 43.9 15.8 3.25 16.94 +3.11

7612 Chicken 5 Tibia 12 43.9 15.6 3.28 17.05 +3.11

7614 Chicken 6 Fibula 2 42.6 15.4 3.24 15.78 +3.82

7615 Chicken 6 Femur 3 43.4 15.3 3.31 15.55 +3.55

7615 Chicken 6 Femur 3 44.4 15.6 3.32 15.60 +3.55

7980 Chicken 6 Scapula 5 44.8 16.1 3.24 15.08 +3.68

8414 Chicken 6 Scapula 6 43.6 15.9 3.20 15.00 +3.66

8415 Chicken 6 Radius 7 44.1 16.1 3.20 14.95 +3.57

8416 Chicken 6 Radius 8 43.3 15.6 3.23 15.05 +3.51

8416 Chicken 6 Radius 8 43.2 15.6 3.23 15.07 +3.46

8417 Chicken 6 Ulna 9 44.4 15.7 3.30 15.08 +3.36

8418 Chicken 6 Ulna 10 44.7 15.8 3.30 15.06 +3.53

8419 Chicken 6 Tibia 11 44.8 15.9 3.28 15.48 +3.54

8421 Chicken 6 Humerus 13 46.3 16.4 3.29 14.82 +3.30

8426 Chicken 7 Ulna 4 45.3 15.9 3.32 16.56 +2.90

8427 Chicken 7 Scapula 5 45.6 16.1 3.30 17.17 +3.16

8428 Chicken 7 Scapula 6 46.1 16.6 3.24 16.78 +3.14

8429 Chicken 7 Femur 7 46.0 16.3 3.30 16.81 +2.92

8429 Chicken 7 Femur 7 46.3 16.4 3.30 16.83 +2.89

8430 Chicken 7 Femur 8 45.2 16.0 3.29 16.70 +3.07

8491 Chicken 7 Radius 9 45.3 16.3 3.24 16.49 +2.99

8637 Chicken 7 Tibia 12 46.5 16.4 3.30 16.96 +3.13

8637 Chicken 7 Tibia 12 46.2 16.3 3.30 16.99 +3.01

8639 Chicken 7 Coracoid 14 46.9 16.5 3.32 16.98 +2.91

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e # # %C %N

8641 Chicken 7 Humerus 16 46.2 16.4 3.28 16.40 +2.74

8642 Chicken 7 Humerus 17 45.2 16.2 3.25 -16.10 +2.74

8642 Chicken 7 Humerus 17 44.8 16.1 3.25 16.12 +2.71

8643 Cow 13 Talus 1 45.4 16.8 3.15 12.43 +5.24

8644 Cow 13 distal tibia 2 44.0 16.3 3.14 11.22 +5.09

8645 Cow 13 Calcaneous 3 44.1 16.3 3.15 11.63 +5.41

8645 Cow 13 Calcaneous 3 43.9 16.3 3.14 11.56 +5.51

8646 Cow 13 Calcaneous epiphysis 4 46.1 17.0 3.16 11.34 +5.40

8647 Cow 13 Navicular 5 46.0 17.0 3.17 10.68 +5.31

8648 Cow 13 Navicular epiphysis 6 45.7 16.8 3.16 12.36 +5.14

8649 Cow 13 Sustenaculum tali 7 45.3 16.7 3.16 9.97 +5.59

8650 Cow 13 Talus 8 45.8 16.9 3.17 14.67 +6.89

8650 Cow 13 Talus 8 46.3 17.1 3.17 14.63 +6.96

8651 Cow 14 Calcaneus 1 44.7 16.5 3.16 12.69 +7.10

8652 Cow 14 Calcaneus epiphysis 2 45.9 16.9 3.16 15.95 +6.94

8653 Cow 14 Sustenaculum tali 3 45.1 16.6 3.17 12.13 +6.90

8654 Cow 14 navicular 4 44.9 16.6 3.16 15.66 +6.81

8655 Cow 14 Navicular epiphysis 5 46.1 16.9 3.18 16.29 +7.04 8655 Cow 14 Navicular epiphysis 5 45.4 16.7 3.17 16.25 +7.13

8656 Cow 14 distal tibia 6 45.3 16.5 3.20 9.65 +7.06

10240 Cow 15 Calcaneus 1 43.9 16.2 3.15 9.56 +5.02

10241 Cow 15 Distal tibia 2 45.9 16.9 3.17 11.89 +5.42

10242 Cow 15 Navicular 3 46.0 17.0 3.16 10.78 +5.30

10243 Cow 15 Calcaneus epiphysis 4 45.8 16.8 3.18 11.37 +5.39 10243 Cow 15 Calcaneus epiphysis 4 45.3 16.7 3.16 11.34 +5.43 10244 Cow 15 Sustenaculum tali 5 45.3 16.7 3.16 11.30 +5.49

10245 Cow 15 Talus 6 45.7 16.8 3.17 12.74 +5.53

10246 Cow 16 Calcaneus 1 45.7 16.8 3.16 16.76 +6.85

10247 Cow 16 distal tibia 2 43.4 15.9 3.18 20.05 +6.23

10248 Cow 16 Navicular 3 43.4 16.0 3.16 12.48 +6.89

10249 Cow 16 Calcaneus epiphysis 4 44.7 16.4 3.19 12.79 +6.96 10250 Cow 16 Navicular epiphysis 5 44.7 16.4 3.18 16.00 +6.90 Sampl Animal Animal Bone Bone# wt wt C:N δ¹³C δ¹⁵N

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e # # %C %N

10250 Cow 16 Navicular epiphysis 5 43.8 16.1 3.18 15.99 +6.89

10251 Cow 16 Talus 6 45.1 16.6 3.17 14.46 +7.04

10252 Cow 17 Calcaneus 1 43.9 15.8 3.23 11.42 +6.79

10253 Cow 17 distal tibia 2 45.1 16.5 3.19 11.55 +6.39

10254 Cow 17 Calcaneus epiphysis 3 42.2 15.6 3.16 11.81 +6.56 10255 Cow 17 Sustenaculum tali 4 43.5 16.0 3.17 11.62 +6.32 10255 Cow 17 Sustenaculum tali 4 43.0 15.8 3.18 11.71 +6.38

10256 Cow 17 Talus 5 42.4 15.5 3.20 11.30 +6.48

10257 Cow 18 Carpal bone II & III fused 1 44.7 16.4 3.18 12.84 +7.49

10258 Cow 18 Carpal bone IV 2 43.7 16.0 3.19 12.79 +7.23

10259 Cow 18 Ulnar carpal bone 3 43.3 15.9 3.18 12.96 +7.33 10260 Cow 18 Intermediate carpal bone 4 42.7 15.6 3.19 13.09 +7.34 10261 Cow 18 Radial carpal bone 5 42.4 15.6 3.18 12.88 +7.19 10262 Cow 18 Accessary Carpal bone 6 42.5 15.6 3.18 13.08 +7.14

10263 Cow 18 Body of Radius 7 38.1 13.9 3.19 12.43 +7.41

10264 Cow 18 Body of Ulna 8 42.1 15.4 3.18 12.98 +6.96

10266 Racoon 20 Ribs #2 2 45.3 16.7 3.16 13.17 +9.17

10266 Racoon 20 Ribs #2 2 45.6 16.8 3.16 13.18 +9.17

10345 Turkey 21 Humerus 1 43.3 15.7 3.22 16.24 +3.95

10346 Turkey 21 Humerus 2 42.6 15.4 3.23 16.27 +4.03

10347 Turkey 21 Femur 3 42.8 15.4 3.25 16.41 +4.17

10348 Turkey 21 Femur 4 43.3 15.4 3.27 16.40 +4.11

10349 Turkey 21 Tibia 5 42.9 15.4 3.26 16.31 +4.04

10349 Turkey 21 Tibia 5 42.7 15.3 3.26 16.33 +4.14

10350 Turkey 21 Tibia 6 42.8 15.3 3.26 16.26 +4.05

10351 Turkey 21 Fibula 7 42.3 15.0 3.29 16.87 +4.18

10352 Turkey 21 Fibula 8 43.0 15.3 3.28 16.83 +4.17

10353 Turkey 21 Sternum 9 43.3 15.5 3.25 16.57 +4.18

10354 Turkey 21 Coracoid 10 42.4 15.5 3.19 16.25 +4.13

10355 Turkey 21 Coracoid 11 43.0 15.6 3.21 16.49 +3.92

10355 Turkey 21 Coracoid 11 42.9 15.7 3.20 16.51 +3.97

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e # # %C %N

10356 Turkey 21 Ulna 12 42.7 15.5 3.22 16.49 +3.92

10357 Turkey 21 Ulna 13 42.9 15.4 3.25 16.30 +4.00

10358 Turkey 21 Scapula 14 42.6 15.2 3.28 16.63 +4.19

10359 Turkey 21 Scapula 15 42.7 15.2 3.28 16.50 +4.07

10360 Turkey 21 Radius 16 43.1 15.6 3.22 16.36 +3.96

10360 Turkey 21 Radius 16 43.2 15.6 3.22 16.33 +3.92

10361 Turkey 21 Radius 17 42.7 15.5 3.21 16.32 +3.89

10502 Cow 19 Intermediate carpal bone 7 41.4 15.2 3.17 21.49 +6.51 12087 Cow 19 Carpal bone II & III fused 1 42.5 15.6 3.19 20.92 +6.71 12088 Cow 19 Radial carpal bone 2 42.4 15.5 3.19 21.60 +6.80

12091 Cow 19 Body of Ulna 5 40.9 15.1 3.17 22.28 +6.69

12093 Raccoon 20 Femur #2 3 45.1 16.6 3.18 14.90 +9.74

12094 Raccoon 20 Sacrum 4 44.8 16.4 3.18 13.10 +9.45

12095 Raccoon 20 Os coxae #2 5 45.4 16.8 3.16 13.52 +8.91

12096 Raccoon 20 Mandible #2 6 42.8 15.9 3.15 14.12 +9.10