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Online Resource 1 Sample size per group

Compound Concentration

(mg/L) Sample Size

Nicotine 0 84

0.3 12

1 27

3 12

Cotinine 0 48

30 12

100 12

300 24

Anatabine 0 29

0.3 12

1 12

3 12

Methylanatabine 0 12

1 12

3 12

10 12

Anabasine 0 12

0.3 12

1 12

3 12

Compound Concentration

(mg/L) Sample Size

Nornicotine 0 20

3 23

10 22

30 22

Metanicotine 0 12

30 12

100 12

300 12

Acetylcholine 0 35

30 12

10 18

300 12

AZD1446 0 22

30 24

100 24

300 24

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