1 8 0 LYMPHORETICULAR CELLS [ 1 8 ]
[18] E l e c t r o p h o r e t i c S e p a r a t i o n o f L y m p h o i d Cells By ERNIL HANSEN and KURT HANNIG
I n t r o d u c t i o n
The dissection of a complex system into its components, their sepa- rate analysis, and the controlled reconstitution is a scientific principle that has been used quite successfully in biochemistry. Its application in immu- nology calls for techniques for large-scale separation of highly pure and functionally intact lymphocyte subpopulations. Since most lymphocyte functions are mediated through their cell surface, a separation based on differences in cell membrane properties, such as surface charge, seems to be especially promising.
Preparative electrophoresis of cells became possible with the develop- ment of free-flow electrophoresis by K. Hanning. 1-3 The application of this method to the separation of lymphocytes is closely connected with the name of our collegue, the late K. Zeiller. He and a number of workers in other laboratories have demonstrated the potential of free-flow electro- phoresis to efficiently separate murine T and B cells, as well as lympho- cyte subpopulations at different stages of activation or differentiation (reviewed in refs. 4-9). Although most investigations have dealt with lymphocytes from mice and rats, lymphoid cells from humans, 5.10-J2 non-
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Copyright ~) 1984 by Academic Press, Inc.
METHODS IN ENZYMOLOGY. VOL. 108 All rights of reproduction in any form reserved.
ISBN 0- ! 2-182008-4
