Supplementary Figure 1. Particle counts during culture experiment for A) total particles B) particles ≥ 0.3 μM, C) particles ≥ 0.5 μM, D) particles ≥ 0.7 μM, E) particles ≥ 1.2 μM, E) particles ≥ 2.0 μM, E) particles ≥ 5.0 μM.

Supplementary Figure 1. Particle counts during culture experiment for A) total particles B) particles ≥ 0.3 μM, C) particles ≥ 0.5 μM, D) particles ≥ 0.7 μM, E) particles ≥ 1.2 μM, E) particles ≥ 2.0 μM, E) particles ≥ 5.0 μM.

Supplementary Figure 2. Particle counts during nucleotide experiments for A) total particles B) particles ≥ 0.3 μM, C) particles ≥ 0.5 μM, D) particles ≥ 0.7 μM, E) particles ≥ 1.2 μM, E) particles ≥ 2.0 μM, E) particles ≥ 5.0 μM

Supplementary Figure 3. DNA yield normalized to volume of air sampled shown with samples grouped corresponding to the instrument used to collect samples.

Supplementary Figure 4. Colony-forming units (CFUs) normalized to volume of air sampled shown as bars on the left y-axis and total particle counts shown as lines on the right y-axis, with each bar representing a plate. The colors correspond to the instrument used to collect samples onto each plate.

0.000 0.005 0.010 0.015 0.020 0.025

EP EPA LI MF

Instrument DNA concentration(ug/mLm-3 air)

0 100 200 300

0 5000 10000 15000

Plate ID

Colony Forming Units (CFUs) m-3 air Total Particle Count

Instrument

Electrostatic Precipitator Liquid Impinger

Membrane Filter