Supplementary Table 2
Table of parameters determined by behavioral tests and nociception assays with WT and SPRED2 KO mice aged 2-4 months without visible grooming phenotype and skin lesions.
Behavioral test Parameter WT KO Significance
OF
Groomings (n) 2.3±0.5 2.4±0.5 n.s.
Total distance (m) 20.4±2.8 20.7±2.5 n.s.
Time in center (%) 3.3±1.1 3.9±2.2 n.s.
Rearings (n) 9.7±3.7 12.4±3.6 n.s.
EPM
Time in open arm (%) 15.8±3.6 25.8±12.2 n.s.
Total distance (m) 16.8±1.2 15.1±0.9 n.s.
Rearings (n) 6.0±0.9 5.4±1.5 n.s.
LDB
Time in light area (%) 44.8±12.5 45.4±10.7 n.s.
Latency to cross (s) 118.1±37.2 117.1±34.2 n.s.
Groomings (n) 0.9±0.5 1.0±0.8 n.s.
Nociception assay Parameter WT KO Significance
von Frey hair Mechanical threshold 0.44±0.11 0.32±0.08 n.s.
Hargreaves Thermal latency 10.04±0.9 9.6±0.8 n.s.
OF: Open field, EPM: Elevated plus maze, LDB: Light/dark box. n=7 for WT and KO in behavioral tests, n=8 for WT and KO in nociception assays. Data are mean ± s.e.m; *P < 0.05, **P < 0.01, ***P < 0.001, n.s.=not significant.
