NTF3. Non-staining control data panels for each transduced cell line are included.

CD133+

0.19

singlet 94.8

FSC-A x FSC-H cells 65.3

FSC-A x SSC-A

0 103

104 105 Comp-R670-A :: CD133-APC 0

50K 100K 150K 200K 250K

FSC-A

af100920_GFP Control-2_003.fcs cells/singlet

41452

CD133+

0.11

singlet 93.5

FSC-A x FSC-H cells 71.0

FSC-A x SSC-A

0 103

104 105 Comp-R670-A :: CD133-APC 0

50K 100K 150K 200K 250K

FSC-A

af100920_GFP Control-3_004.fcs cells/singlet

48259

CD133+

0.27

singlet 92.1

FSC-A x FSC-H cells 57.5

FSC-A x SSC-A

0 103

104 105 Comp-R670-A :: CD133-APC 0

50K 100K 150K 200K 250K

FSC-A

af100920_GFP Control-4_005.fcs cells/singlet

35800

CD133+

0.24

singlet 92.8

FSC-A x FSC-H cells 57.4

FSC-A x SSC-A

0 103

104 105 Comp-R670-A :: CD133-APC 0

50K 100K 150K 200K 250K

FSC-A

af100920_GFP NTF3-5_006.fcs cells/singlet 36204

CD133+

0.11

singlet 94.3

FSC-A x FSC-H cells 80.7

FSC-A x SSC-A

0 103

104 105 Comp-R670-A :: CD133-APC 0

50K 100K 150K 200K 250K

FSC-A

af100920_GFP NTF3-6_007.fcs cells/singlet 53448

CD133+

0.20

singlet 93.2

FSC-A x FSC-H cells 56.1

FSC-A x SSC-A

0 103

104 105 Comp-R670-A :: CD133-APC 0

50K 100K 150K 200K 250K

FSC-A

af100920_GFP NTF3-7_008.fcs cells/singlet 35436

CD133+

0.17

singlet 80.2

FSC-A x FSC-H cells 87.0

FSC-A x SSC-A

0 103

104 105 Comp-R670-A :: CD133-APC 0

50K 100K 150K 200K 250K

FSC-A

af100920_GFP Unstained-1_001.fcs cells/singlet

28262

CD133+

0.21

singlet 93.2

FSC-A x FSC-H cells 68.0

FSC-A x SSC-A

0 103

104 105 Comp-R670-A :: CD133-APC 0

50K 100K 150K 200K 250K

FSC-A

af100920_SLITRK3 Control-9_009.fcs cells/singlet

44173

CD133+

0.22

singlet 94.4

FSC-A x FSC-H cells 55.4

FSC-A x SSC-A

0 103

104 105 Comp-R670-A :: CD133-APC 0

50K 100K 150K 200K 250K

FSC-A

af100920_SLITRK3 Control-10_010.fcs cells/singlet

48923

CD133+

0.24

singlet 90.9

FSC-A x FSC-H cells 84.5

FSC-A x SSC-A

0 103

104 105 Comp-R670-A :: CD133-APC 0

50K 100K 150K 200K 250K

FSC-A

af100920_SLITRK3 Control-11_011.fcs cells/singlet

56905

CD133+

0.40

singlet 94.5

FSC-A x FSC-H cells 30.7

FSC-A x SSC-A

0 103

104 105 Comp-R670-A :: CD133-APC 0

50K 100K 150K 200K 250K

FSC-A

af100920_SLITRK3 NTF3-13_012.fcs cells/singlet

25208

CD133+

1.36

singlet 91.8

FSC-A x FSC-H cells 20.0

FSC-A x SSC-A

0 103

104 105 Comp-R670-A :: CD133-APC 0

50K 100K 150K 200K 250K

FSC-A

af100920_SLITRK3 NTF3-14_001_014.fcs cells/singlet

13520

CD133+

1.25

singlet 88.5

FSC-A x FSC-H cells 24.6

FSC-A x SSC-A

0 103

104 105 Comp-R670-A :: CD133-APC 0

50K 100K 150K 200K 250K

FSC-A

af100920_SLITRK3 NTF3-14_013.fcs cells/singlet

33616

CD133+

0.27

singlet 83.4

FSC-A x FSC-H cells 52.5

FSC-A x SSC-A

0 103

104 105 Comp-R670-A :: CD133-APC 0

50K 100K 150K 200K 250K

FSC-A

af100920_SLITRK3 Unstained-8_002.fcs cells/singlet

13145

Supplementary Fig. 1. Density plots from FACS analysis of CD133-positive cell fractions compiled from 3 independent biological replicates

for each of 4 groups: H226 cells transduced with GFP plus and minus NTF3, and H226 cells transduced with SLITRK3 plus and minus

NTF3. Non-staining control data panels for each transduced cell line are included.