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coli NovaBlue (Novagen) used as general cloning strain was grown in Luria broth (Sambrook, 2001), supplemented with 100 µg/mL apramycin to maintain plasmidsE.
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and TPC) determined with two different techniques: (1) carbon concentration using an elemental analyzer, and (2) calcium concentration using ICP-AES, and results were compared.
On one hand, PIC determination using EA requires an intermediate step of acidification (POC samples are fumed with H 2 SO 4 in order to dissolve inorganic carbon in the samples),
Supporting information.
Pearson product-moment correlation coefficients between all parameters measured on the 13 different Emiliania huxleyi strains cultured under identical environmental conditions..
The values in the upper right of each plot show the number of El Niño winters included in each calculation, and the associated anomaly correlation coefficient
Environmental characteristics (temperature, pH, dissolved oxygen (D.O.), and salinity) of three ballast water (A, B, and C) samples obtained at the initial (int), middle (mid),