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S3 File. Biological activity of biotinylated gephyronic acid methyl ester

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S3 File. Biological activity of biotinylated gephyronic acid methyl ester

(A) (B)

Figure: Growth and translation inhition of biotinylated gephyronic acid methyl esters.

(A) Measuring the concentration dependent metabolic activity of KB-3-1 cell cultures that were incubated with 4a and 4b by an MTT assay showed that the compounds still had a high inhibiting capacity. 4b was the more active one. It was almost as cytotoxic to KB-3-1 cells as the parent compound 1. The IC50 was 0.06 µM. Assays were run in duplicates. Error bars show S.D. (B) 4b also inhibited in vitro translation (IC50 6 µM) in a rabbit reticulocyte lysate system supplemented with mRNA and amino acids. Experiment was run in triplicates. Error bars show S.D.

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4b

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Luminescence / %

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