Electronic Supplement 2

Electronic Supplement 2

1 2 3 4 MXID

Diaponta anfibia 0.93 1.23

Diapontia niveovittata 2.41 1.15 2.50

Diapontia uruguayensis 2.48 1.03 0.89 1.24 Diapontia securifera 2.97 4.14 4.00 0.00 0.00

Table S2.1 Summary of the genetic distances of Diapontia species, numbers on the middle diagonal line correspond to the intraspecific variations.

BARCODE Gap analysis

Species Mean Intra-

Sp Max Intra-

Sp Nearest Species Nearest

Neighbour Distance to NN

Diapontia niveovittata 1.15 2,5 Diapontia uruguayensis SPDAR288-13 0

Diapontia securifera 0 0 Diapontia anfibia SPDAR944-15 2,81

Diapontia uruguayensis 0.89 1,24 Diapontia niveovittata SPDAR323-14 0

Diapontia anfibia 0.93 1,23 Diapontia niveovittata SPDAR243-13 1,86

Table S2.2 Genetic divergence values for each species, the mean (MID) and maximum intra-specific values are compared to the nearest neighbor.

Fig. S2.3 A plot of the maximum intraspecific distance compared with distance to

nearest neighbor of all species of the genus Diapontia used in this study.

BOLD Museum code Species Country Provice/Region CORAR057-13 MACN-Ar 30309 Diapontaa niveovittata Argentnn Entre Ríos SPDAR234-13 MACN-Ar 30519 Diapontaa niveovittata Argentnn Entre Ríos SPDAR236-13 MACN-Ar 30520 Diapontaa niveovittata Argentnn Entre Ríos SPDAR243-13 MACN-Ar 28615 Diapontaa niveovittata Argentnn Snntn Fe SPDAR323-14 MACN-Ar 30908 Diapontaa niveovittata Argentnn Snntn Fe SPDAR337-14 MACN-Ar 30903 Diapontaa niveovittata Argentnn Snntn Fe SPDAR339-14 MACN-Ar 30906 Diapontaa niveovittata Argentnn Snntn Fe SPDAR382-14 MACN-Ar 31095 Diapontaa niveovittata Argentnn Corrientes SPDAR814-14 MACN-Ar 32187 Diapontaa niveovittata Argentnn Snntn Fe SPDAR857-14 MACN-Ar 31348 Diapontaa niveovittata Argentnn Snntn Fe MULAD295-16 MACN-Ar 34767 Diapontaa niveovittata Argentnn Snn Junn

SPDAR926-15 MACN-Ar 31188 Diapontaa securifera Chile Aricn y Pnrinncotn SPDAR928-15 MACN-Ar 31190 Diapontaa securifera Chile Aricn y Pnrinncotn SPDAR258-13 MACN-Ar 28610 Diapontaa uruguayen si s Argentnn Buenos Aires SPDAR1437-16 MACN-Ar 35787 Diapontaa uruguayen si s Argentnn Buenos Aires SPDAR288-13 MACN-Ar 30342 Diapontaa uruguayen si s Argentnn Río Negro SPDAR287-13 MACN-Ar 30343 Diapontaa uruguayen si s Argentnn Río Negro SPDAR610-14 MACN-Ar 31393 Diapontaa uruguayen si s Uruguny Mnldonndo

SPDCH354-13 MACN-Ar 28612 Diapontaa anfiia Chile Bío-Bío

SPDCH355-13 MACN-Ar 28611 Diapontaa anfiia Chile Bío-Bío

SPDAR944-15 MACN-Ar 34444 Diapontaa anfiia Argentnn Chubut

SPDAR989-15 MACN-Ar 34761 Diapontaa anfiia Argentnn Rio Negro

Table S2.4 List of specimens of Diapontia used on the BARCODE DNA analysis.

Fig. S2.5 Tree obtained under maximum likelihood analysis, values on the nodes

indicate the boostrap values.