Induction of Hydroxycinnamic Acid Amides and Tryptophan by Jasmonic Acid, Abscisic Acid and Osmotic Stress in Barley Leaves
Yuki Ogura, Atsushi Ishihara* and Hajime Iwamura
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606Ð8502, Japan; and CREST, Japan Science and Technology Corporation (JST), Japan.
Fax: +81-75-753-6408. E-mail: aishiha@kais.kyoto-u.ac.jp
* Author for correspondence and reprint request
Z. Naturforsch.56c,193Ð202 (2001); received October 19/December 11, 2000 Jasmonic Acid, Abscisic Acid, Hydroxycinnamic Acid Amide
The effects of jasmonic acid (JA) and abscisic aid (ABA) on secondary metabolism in barley (Hordeum vulgareL.) were investigated. Treatment with JA at 100µmfor 48 h induced accumulation of four compounds in barley primary leaves. The accumulation of these com- pounds was also observed after treatment with ABA at 100µm. The induced compounds were identified asp-coumaroylputrescine,p-coumaroylagmatine,p-coumaroyl-3-hydroxyagmatine and tryptophan by spectroscopic methods. The profiles of compounds induced by application of JA and ABA were different. JA exhibited stronger inducing activity for hydroxycinnamic acid amides than ABA, while ABA was more active in tryptophan accumulation. The major hydroxycinnamic acid amides in JA- and ABA-treated leaves werep-coumaroylagmatine and p-coumaroyl-3-hydroxyagmatine, respectively. These differences suggested that JA and ABA act in distinct modes. The induction of these compounds was also observed in leaf segments treated with 1msorbitol and glucose. These findings suggested that JA and ABA are involved in accumulation of hydroxycinnamic acid amides and tryptophan in response to osmotic stress in barley.
