9. Appendix
9.6. Supplementary tables
Supplementary Table S 4-1. Identified proteins excised from corresponding regions at
Coomassie stained preparative 2D gel with MDC from cow2 and cow 3. ... 85 Supplementary Table S 4-2. Proteins quantified by 2D DIGE and identified by 2D GE
and subsequent MALDI-TOF analysis... 88 Supplementary Table S 4-3. Proteins identified in MDC (97), as detected by GeLC-MS. ... 91 Supplementary Table S 4-4. Proteins identified in CDC (129), as detected by
GeLC-MS. ... 93 Supplementary Table S 4-5. Proteins identified in each MDC and CDC (521),
respectively, as detected by LC-orbitrap-MS/MS... 96 Supplementary Table S 4-6. Proteins solely detected in MDC of both strains (41), as
detected by LC-orbitrap-MS/MS... 99 Supplementary Table S 9-1. Identified proteins excised from corresponding regions at
Coomassie stained preparative 2D gel with MDC from cow2... 148
Supplementary Table S 9-2. Identified proteins excised from corresponding regions at
Coomassie stained preparative 2D gel with MDC from cow3... 150 Supplementary Table S 9-3. Identified proteins excised from corresponding regions at
Coomassie stained preparative 2D gel with MDC from cow3 (technical repeat of 2D GE/2D DIGE from Fig. 2a, b and Table 2). ... 153 Supplementary Table S 9-4. Identified proteins excised from corresponding regions at
Coomassie stained preparative 2D gel with MDC from cow2... 156 Supplementary Table S 9-5. Identified proteins excised from corresponding regions at
Coomassie stained preparative 2D gel with MDC from cow3... 159 Supplementary Table S 9-6. Identified proteins excised from corresponding regions at
Coomassie stained preparative 2D gel with MDC from cow2... 162 Supplementary Table S 9-7. Identified proteins excised from corresponding regions at
Coomassie stained preparative 2D gel with MDC from cow3... 167
Appendix
174 9.7. Complete list of own publications:
Research articles:
Weigoldt, M., Meens, J., Doll, K., Fritsch, I., Möbius, P., Goethe, R. & Gerlach, G. F.
(2011). Differential proteome analysis of Mycobacterium avium subsp.
paratuberculosis grown in vitro and isolated from cases of clinical Johne's disease.
Microbiology 157, 557-565
Oral presentation at scientific meetings and conferences:
Weigoldt, M., Meens, J., Doll, K., Fritsch, I., Möbius, P., Goethe, R. & Gerlach, G. F.
(2010). Proteome analysis of Mycobacterium avium subsp. paratuberculosis grown in vitro and isolated from cases of clinical Johne’s disease.
Jahrestagung Deutsche Veterinämedizinische Gesellschaft (DVG) - Fachgruppe Bakteriologie und Mykologie und des Friedrich-Loeffler Instituts (Jena, Germany)
Poster presentation at scientific meetings and conferences:
Weigoldt, M., Meens, J., Doll, K., Goethe, R. & Gerlach, G. F. (2009). Mycobacterium avium subsp. paratuberculosis metabolome and antigen expression in vivo.
Nationales Symposium für Zoonosenforschung (Berlin, Germany)
Weigoldt, M., Bargen, I., Weiß, S., Goethe, R. & Gerlach, G. F. (2009).The construction of a
"cherry"-red indicator strain of Mycobacterium avium subsp. paratuberculosis.
Veterinary Network of Laboratories Researching into Improved Diagnosis and Epidemiology of Mycobacterial Diseases (VENoMYC) – Final Meeting (Turin, Italy)
Weigoldt, M., Meens, J., Doll, K., Gerlach, G. F. (2009). Differential proteome analysis (in vitro vs. in vivo) of Mycobacterium avium ssp. paratuberculosis.
10th International Colloquium on Paratuberculosis (Minneapolis, Minnesota, USA)
Weigoldt, M., Doll, K., Goethe, R. & Gerlach, G. F. (2010). Differential proteome analysis (in vitro vs. in vivo) of Mycobacterium avium ssp. paratuberculosis.
3rd Joint Conference: German Society for Hygiene and Microbiology 62. Jahrestagung der Deutschen Gesellschaft für Hygiene und Mikrobiologie (DGHM) & Association for General and Applied Microbiology Jahrestagung der Vereinigung für Allgemeine und Angewandte Mikrobiologie (VAAM), (Hannover, Germany)
Acknowledgements
First and foremost, I would like to sincerely thank my advisor Prof. Dr. Gerald-F. Gerlach. He encouraged me to work on this interesting topic and shared his enthusiasm for Mycobacterium avium subsp. paratuberculosis with me. In spite of all responsibilities, he spent lots of time in the lab sharing his practical experiences and providing guidance with limitless amount of patience and optimism. Furthermore, I wish to express my gratitude to Prof. Dr. Ralph Goethe for continuing the excellent supervision and his sustained interest and participation in my work. Both Prof. Dr. Gerald-F. Gerlach and Prof. Dr. Ralph Goethe, contributed equally in supervising me, always leaving their door open and taking time to patiently listen to any of my questions.
I would like to thank my co-supervisors Prof. Dr. Mathias Hornef and Prof. Dr. Andreas Beineke for their interest in my work, their ideas and productive discussions, and the external expert, Dr.Siegfried Weiß, for taking the time to read my thesis.
This work would not have been possible without the expertise and efforts of my collaborators.
I would like to thank Prof. Dr. Klaus Doll from the Justus-Liebig-University in Giessen, for providing me with cows with clinical Johne’s disease and the Department of Pathology in Giessen for preparing intestinal pieces from these animals. For genomic strain typing of the Mycobacterium avium subsp. paratuberculosis isolates, I would like to thank Petra Möbius and Isabel Fritsch from the Friedrich-Loeffler-Institute in Jena. For LC-orbitrap-MS and MALDI-TOF/TOF analysis of remaining, previously “not significant” spot identifications I would like to thank Dr. Jochen Meens and Prof. Dr. Andreas Pich from the MS lab in the Institute for Toxicology at Medical School Hannover.
Special thanks go to Dr. Falk Büttner and Dr. Jochen Meens for the guidance in technical aspects of protein analysis methods and Ute Regul, who provided technical assistance during protein spot processing for MS-analysis. Furthermore, I would like to thank Dr. Tina Basler for the excellent organisation of the ZooMAP meetings and all past and present members of the ZooMAP project for great collaborations.
I would like to thank the head of the Department, Prof. Dr. Peter Valentin-Weigand, for the good atmosphere at the Institute for Microbiology and all the past and present members of the
Institute for Microbiology, for an excellent collegial and productive working atmosphere, subject-related discussions and lots of fun during lunch time and coffee breaks: Dr. Tina Basler, Tanja Bauer, Sabine Baumert, PD Dr. Christoph Baums, Dr. Ibrahim Bendallah, Nadine and Dr. Falk Büttner, Sabrina Diehl, Dr. Karla Dreckmann, Anna Drees, Elke Eckelt, Dr. Marcus Fulde, Sabine Göbel, Dr. Petra Grüning, Nina Janze, Dr. Dennis Kahlisch, Dr.
Jochen Meens, Kristin Laarmann, Thorsten Meißner, Dr. Andreas Mietze, Dr. Franziska Müller, Dr. Shamoon Naseem, Dr. Andreas Nerlich, Dr. Girish Ramachandran, Dr. Thomas Rehm, Dr. Meike Sack, Julian Sander, Franziska Schill, Jana Seele, Dr. Martin Selke, Yenehiwot Berhanu Weldearegay, Dr. Joerg Willenborg and Daniela Willms.
Many thanks go to Jörg Merkel for helping me with all technical questions concerning computer and MS Excel solutions which were very helpful for my data evaluation.
I am grateful for always having the support of my girlfriend Susi.
Finally and most importantly, I would like to thank my parents, brothers and sister and their beloved families, as well as my grandfather for their constant support and encouragement over all these years, and keeping their hearts open for me.