5. Chapter: General discussion
5.5. Conclusions and Outlook
Technological advances in the field of proteomic research such as high-throughput analysis of proteins using LC-MS/MS enabled us to provide large data sets of proteins expressed by MAP during clinical stages of disease in naturally infected cows. Moreover, proteins significantly higher expressed in two mucosa-derived MAP strains compared to their respective culture-derived counterparts are assumed to provide molecular hints to the physiological adaptation during the intracellular life cycle of these mycobacteria. Based on the results we hypothesized that the metabolic activity in the host is based of a “shared principle type” using a large variety of substrates available.
Despite the progress, we are still at the beginning of understanding the complex interplay between host factors inducing bacterial protein expression and their contribution to pathogenesis of mycobacterial infections. Nevertheless, this study provides strong the confirmatory evidence for many aspects of in vivo-metabolism of MAP and other pathogenic mycobacteria, especially M. tuberculosis, previously only obtained from cell culture and mouse infection experiments. Also, we could confirm and expand data obtained by other
groups investigating gene and protein expression of MAP during natural infection or exposure to host-related stressors such as hypoxia, oxidative or nitrosative burst and nutrient starvation.
Furthermore, this study provides novel targets for future work in diagnostics and vaccination.
In the future additional studies have do be performed in order to prove our hypothesis of a
“shared principle type” metabolism of MAP during growth inside the host, and the potential diagnostic value of the antigens has to be investigated.
Chapter 5 - General discussion
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Chapter 6
Summary
6. Chapter: Summary