Nucleotide sequence
1 ATGGAGCTGA TTGTGCTGTT GATCCTCCTG AATCCCTATA CCTTCGTTCT GGGCGACCGG 61 ATTTGCATTG GATATCAGGC TAACCAGAAC AATCAGACCG TCAACACACT GCTGGAGCAA 121 AATGTCCCTG TGACCGGGGC TCAAGAGATC CTGGAGACAA ACCACAATGG GAAATTGTGT 181 AGTCTCAACG GCGTTCCTCC ACTGGATCTG CAGTCCTGTA CTCTTGCAGG CTGGTTGCTC 241 GGAAATCCCA ACTGTGACTC CTTGCTGGAG GCAGAAGAGT GGAGCTACAT CAAGATCAAC 301 GAGTCTGCCC CTGACGATCT GTGTTTTCCT GGCAATTTTG AAAACCTCCA AGATCTGTTG 361 CTGGAAATGT CTGGCGTCCA GAATTTCACA AAGGTAAAGC TGTTCAATCC ACAGAGTATG 421 ACCGGCGTGA CAACCAATAA CGTGGACCAA ACATGTCCCT TTGAGGGTAA ACCCAGCTTT 481 TATCGGAATC TGAATTGGAT TCAGGGTAAC AGCGGACTTC CCTTCAATAT CGAAATCAAG 541 AATCCAACGT CCAATCCCCT GCTCCTGCTT TGGGGTATTC ACAACACAAA GGACGCGGCT 601 CAACAACGGA ATCTGTATGG GAACGACTAC AGCTATACGA TTTTCAACTT TGGGGAGAAG 661 AGCGAGGAGT TCAGACCAGA AATTGGCCAG AGAGATGAAG TGAAAGCCCA TCAGGACAGG 721 ATCGATTACT ACTGGGGTAG CCTGCCGGCC CAATCTACTC TCAGGATCGA AAGCACTGGC 781 AACCTGATTG CGCCTGAGTA CGGGTTCTAC TACAAACGTA AGGAAGGCAA AGGAGGGCTT 841 ATGAAAAGTA AGTTGCCCAT ATCAGATTGC TCAACTAAGT GTCAGACACC ACTCGGGGCC 901 CTGAACTCCA CACTGCCTTT TCAGAATGTG CATCAGCAGA CCATTGGAAA CTGCCCGAAA 961 TACGTCAAAG CCACATCTCT TATGCTTGCT ACTGGACTGA GGAACAATCC ACAGATGGAG 1021 GGTAGAGGCC TGTTTGGGGC AATTGCCGGT TTCATAGAAG GTGGGTGGCA GGGCATGATC 1081 GATGGGTGGT ATGGATATCA CCATGAGAAC CAAGAAGGAT CAGGATATGC AGCCGATAAA 1141 GAGGCGACTC AGAAGGCTGT TGATGCCATC ACCAACAAGG TGAACAGCAT CATAGACAAG 1201 ATGAATAGCC AGTTCGAATC CAACATAAAA GAGTTTAACC GCTTGGAACT GCGCATTCAG 1261 CACCTTTCAG ATCGCGTAGA CGATGCCTTG CTGGATATCT GGAGCTATAA TACCGAGCTG 1321 CTGGTACTCC TCGAAAACGA ACGAACTCTG GACTTTCATG ACGCAAACGT TAAGAATCTG 1381 TTCGAGAAAG TGAAGGCTCA GCTGAAAGAT AACGCCATTG ACGAAGGGAA TGGCTGCTTC
1441 CTCCTCCTCC ACAAGTGCAA CAATTCATGC ATGGACGATA TCAAGAATGG CACCTACAAG 1501 TATATGGACT ATAGAGAGGA GAGTCACATA GAGAAGCAGA AAATCGACGG AGTGAAGCTC 1561 ACCGACTACA GTCGATACTA CATCTTAATT AAGTCTAGAG TCGACTGTTT AAACCTGCAG 1621 GCATGCAAGC TTGATATCAT CGAAGGTCGT GGTGGTGGTG GTGGTGATCC CAAATCTTGT 1681 GACAAACCTC ACACATGCCC ACTGTGCCCA GCACCTGAAC TCCTGGGGGG ACCGTCAGTC 1741 TTCCTCTTCC CCCCAAAACC CAAGGACACC CTCATGATCT CCCGGACCCC TGAGGTCACA 1801 TGCGTGGTGG TGGACGTGAG CCACGAAGAC CCTGAGGTCA AGTTCAACTG GTACGTGGAC 1861 GGCGTGGAGG TGCATAATGC CAAGACAAAG CCGCGGGAGG AGCAGTACAA CAGCACGTAC 1921 CGTGTGGTCA GCGTCCTCAC CGTCCTGCAC CAGGACTGGC TGAATGGCAA GGAGTACAAG 1981 TGCAAGGTCT CCAACAAAGC CCTCCCAGCC CCCATCGAGA AAACCATCTC CAAAGCCAAA 2041 GGGCAGCCCC GAGAACCACA GGTGTACACC CTGCCCCCAT CCCGGGATGA GCTGACCAAG 2101 AACCAGGTCA GCCTGACCTG CCTAGTCAAA GGCTTCTATC CCAGCGACAT CGCCGTGGAG 2161 TGGGAGAGCA ATGGGCAGCC GGAGAACAAC TACAAGGCCA CGCCTCCCGT GCTGGACTCC 2221 GACGGCTCCT TCTTCCTCTA CAGCAAGCTC ACCGTGGACA AGAGCAGGTG GCAGCAGGGG 2281 AACGTCTTCT CATGCTCCGT GATGCATGAG GCTCTGCACA ACCACTACAC GCAGAAGAGC 2341 CTCTCCCTGT CTCCGGGTAA ATGA
Amino acid sequence
1 MELIVLLILL NPYTFVLGDR ICIGYQANQN NQTVNTLLEQ NVPVTGAQEI LETNHNGKLC 61 SLNGVPPLDL QSCTLAGWLL GNPNCDSLLE AEEWSYIKIN ESAPDDLCFP GNFENLQDLL 121 LEMSGVQNFT KVKLFNPQSM TGVTTNNVDQ TCPFEGKPSF YRNLNWIQGN SGLPFNIEIK 181 NPTSNPLLLL WGIHNTKDAA QQRNLYGNDY SYTIFNFGEK SEEFRPEIGQ RDEVKAHQDR 241 IDYYWGSLPA QSTLRIESTG NLIAPEYGFY YKRKEGKGGL MKSKLPISDC STKCQTPLGA 301 LNSTLPFQNV HQQTIGNCPK YVKATSLMLA TGLRNNPQME GRGLFGAIAG FIEGGWQGMI 361 DGWYGYHHEN QEGSGYAADK EATQKAVDAI TNKVNSIIDK MNSQFESNIK EFNRLELRIQ 421 HLSDRVDDAL LDIWSYNTEL LVLLENERTL DFHDANVKNL FEKVKAQLKD NAIDEGNGCF 481 LLLHKCNNSC MDDIKNGTYK YMDYREESHI EKQKIDGVKL TDYSRYYILI KSRVDCLNLQ
541 ACKLDIIEGR GGGGGDPKSC DKPHTCPLCP APELLGGPSV FLFPPKPKDT LMISRTPEVT 601 CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK 661 CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE 721 WESNGQPENN YKATPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS 781
LSLSPGK-Table 8.1: Abbreviations of amino acids
A Ala Alanine
C Cys Cysteine
D Asp Aspartic acid
E Glu Glutamic acid
F Phe Phenylalanine
G Gly Glycine
H His Histidine
I Ile Isoleucine
K Lys Lysine
L Leu Leucine
M Met Methionine
N Asn Asparagine
P Pro Proline
Q Gln Glutamine
R Arg Arginine
S Ser Serine
T Thr Threonine
V Val Valine
W Trp Tryptophane
Y Tyr Tyrosine
149
I herewith declare that I autonomously carried out the PhD-thesis entitled
"Analysis of the sialic acid binding activity of the hemagglutinins of influenza viruses and its role in host tropism"
No third party assistance has been used. I did not receive any assistance in return for payment by consulting agencies or any other person. No one received any kind of payment for direct or indirect assistance in correlation to the content of the submitted thesis.
I conducted the project at the following institution:
Institute of Virology, University of Veterinary Medicine Hannover.
The thesis has not been submitted elsewhere for an exam, as thesis or for evaluation in a similar context.
I hereby affirm the above statements to be complete and true to the best of my knowl-edge.
21.02.2013, Anne-Kathrin Sauer
150
I am particulary grateful to Prof. Dr. Georg Herrler for providing this very interesting topic. Thank you for the helpful and inspiring discussions, the support throughout the years and for the opportunity to realize my own ideas.
Prof. Dr. Silke Rauentschlein and Prof. Dr. Rita Gerardy-Schahn I would like to thank for interesting discussions and helpful remarks during our meetings and for evaluating this thesis.
Furthermore I thank Prof. Dr. Wolfgang Garten for external review of this work.
I thank Prof. Dr. Jürgen Stech for providing the H9 cDNA, Prof. Dr. Ben Peeters for providing the cDNA of H7 and H5 and Prof. Dr. Thorsten Wolff who provided the cDNA of the 1918 H1, 2009 H1 and the porcine H1.
Dr. Chi-Hui James Liang I would like to thank for the glycan array work.
I am very grateful to Dr. Maren Bohm and Dr. Meike Erdt who have performed the cloning of H7Fc and H1_2009Fc/H1_1918Fc respectively and who had always an open ear for my problems. We had a great time and I am happy that we became friends even outside the lab.
151
Markus Hoffmann I would like to thank for much appreciated ideas and help but espe-cially for the fun we had, that made the time spent in the lab so much better.
I would also like to thank recent and former members of our lab for the nice working environment and fun inside and outside of the lab, on conferences and courses. In particular I thank Anna, Ann-Kathrin, Christine, Fandan and Yang Wei, Jana, Katarina, Karim, Markus, Martina, Moni, Nadine, Nicole, Sabine, Sarah, Sandra B, Sandra S, Trust and Tim.
I also want to thank my fiancé Mathias for being always there for me through ups and downs and for his patience especially in the last year. I can always rely on you.
In the end my utmost gratitude belongs to my parents without whom I could not have done this. Without your support and constant faith in me I would not be where and who I am today. Thank you!