During their life cycle, enteropathogenic Yersiniae are exposed to different environments inside and outside host organisms. Therefore, they have to adapt their gene expression depending on the condition in order to persist.
The overall goal of this study was to investigate the influence of environmental conditions on the transcriptional landscape of enteropathogenic Yersinia species. To address this question, RNA-seq should be applied. This approach allows the investigation of global changes in gene expression in response to environmental signals as well as the role of non-coding RNAs (ncRNAs) for Yersinia virulence.
For Y. pseudotuberculosis this approach has been used in a previous study (Nuss et al., 2015). This has gained a deep insight into the gene expression profile and has lead to the discovery of new ncRNAs. Here, tissue-dual RNA-sequencing of Y. pseudotuberculosis in mouse Peyer’s Patches was applied to investigate changes between in vitro and in vivo conditions. One aim of this study was the analysis of a target set of genes and ncRNAs that were found to be upregulated in vivo. These targets should be investigated further to identify their role during the infection process. For this purpose, deletion mutants should be created and analyzed.
Another aim of this study was to compare the transcription profile of two Y. enterocolitica serotypes in response to temperature and nutrient availability. Most cases of Yersiniosis in the EU and the USA are caused the bioserotype 4/O:3 (YeO:3) (Bottone, 1999; Bucher et al., 2008; Rosner et al., 2010). However, the bioserotype 1B/O:8 (YeO:8), highly mouse virulent and commonly used in laboratory experiments, is responsible for only 1% of the Yersiniosis cases in Germany (Rosner et al., 2010). Previous studies have shown that both, YeO:3 and YeO:8 are able to colonize mice. But while the YeO:3 infected mice stay clinically healthy, the ones infected with YeO:8 show severe pathological changes (Schaake et al., 2014). On the other hand, YeO:3 was shown to be able to colonize pigs, in contrast to YeO:8 (Schaake et al., 2014). This work aims to investigate the differences of both serotypes on the transcriptional level to gain insights into the mechanisms underlying the different colonization phenotypes. To address this, RNA-seq of Y. enterocolitica at different in vitro conditions should be established. The results should be used to analyze changes in the transcriptional profile on a global level. Gene expression and the presence of ncRNAs should be investigated and a global map of transcriptional start sites for Y. enterocolitica should be created.
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