Steffen Backert
PUBLICATIONS (until 12. 01. 2008)
I Dissertation (PhD thesis)
"Structure and replication of the mitochondrial genome of Chenopodium album"
at the Institute for Biology at Humboldt University Berlin/Germany Supervisor: Prof. Dr. T. Börner;
Grade: Magna cum laude ("Very Good") II Habilitation thesis
"Function of the Helicobacter pylori CagA protein in signal transduction"
Institute for Medical Microbiology, Otto-von-Guericke-University Magdeburg
Director: Prof. Dr. Wolfgang König
III Original papers and reviews in peer-reviewed journals
(listed according to Impact Factor, http://portal.isiknowledge.com/portal.cgi/)
1.) Kwok, T., Zabler, D., Urman, S., Rohde, M., Hartig, R., Wessler, S., Misselwitz, R., Berger, J., Sewald, N., König, W., Backert, S. (2007) Helicobacter pylori exploits integrin for type IV secretion and kinase activation. Nature 449, 862-866. (Impact: 29.274)
2.) Backert, S., Feller, S.M., Wessler, S. (2008) Role of Abl family tyrosine kinases in microbial pathogenesis. Trends in Biochemical Sciences (in press). (Impact: 13.863)
3.) Tammer, I., Brandt, S., Hartig, R., König, W., Backert, S. (2007) Activation of c-Abl kinase by Helicobacter pylori: a novel kinase of the CagA protein and crucial mediator of host cell scattering. Gastroenterology 132, 1309-1319. (Impact: 13.092)
4.) Selbach, M., Moese, S., Hauck, C.R., Meyer, T.F., Backert, S. (2003) The H. pylori CagA protein induces cortactin dephosphorylation and actin cytoskeletal rearrangements by inactivation of c-Src. EMBO Journal 22: 515-528. (Impact: 10.493)
5.) Backert, S. (2002) R-loop dependent rolling-circle replication and a new model for DNA concatemer resolution by mitochondrial plasmid mp1. EMBO Journal 21: 3128-3136.
(Impact: 10,493) 6.) Brandt, S., Kwok, T., Hartig, R., König, W., Backert, S. (2005) NF-κB-activation and potentiation of proinflammatory responses by the Helicobacter pylori CagA protein.
Proceedings of the National Academy of Sciences USA 102: 9200-9205.
(Impact: 10.452)
7.) Backert, S., Nielsen, B.L., Börner, T. (1997) The mystery of the rings: structure and replication of the mitochondrial genome in higher plants. Trends in Plant Science 2: 477- 483. (Impact: 9.701)
8.) Dossumbekova, A., Prinz, C., Gerhard, M., Brenner, L., Backert, S., Kusters, J.G., Schmid, R.M., Rad, R. (2006) Helicobacter pylori outer membrane proteins and gastric inflammation. Gut 55: 1360-1361. (Impact: 9.002)
9.) Selbach, M., Backert, S. (2005) Cortactin: an Achilles´ heel of the actin cytoskeleton.
Trends in Microbiology 13: 181-189. (Impact: 8.335)
10.) Backert, S., Selbach, M. (2005) Tyrosine-phosphorylated microbial effector proteins:
the enemies within. Trends in Microbiology 13: 476-484. (Impact: 8.335)
11.) Backert, S., Meyer, T.F. (2006) Type IV secretion systems and their effector proteins in bacterial pathogenesis. Current Opinion in Microbiology 9: 207-217. (Impact: 8.005)
12.) Backert, S., Dörfel, P., Lurz, R., Börner, T. (1996) Rolling circle replication in the mitochondria of the higher plant Chenopodium album (L.). Molecular and Cellular Biology 16: 6285-6294. (Impact: 7.093)
13.) Gandhi, M., Hammett, L., Scissum-Gunn, K., Backert, S., Nielsen, B.L.
(1998)Analysis of genome structure and gene copy number in Brassica campestris mitochondrial DNA. FASEB Journal 12: 261-262. (Impact: 7.064)
14.) Krause, M., Rohde, M., Hartig, R., Genth, H., Miller, W.G., Schmidt, G., König, W., M.E. Konkel, Backert, S. (2007) Role of the small Rho GTPases Rac1 and Cdc42 in host cell invasion of Campylobacter jejuni. Cellular Microbiology 9,2431-2444.
(Impact: 6.333)
15.) Moese, S., Selbach, M., Brinkmann, V., Karlas, A., Haimovich, B., Backert, S., Meyer, T.F. (2007) The H. pylori CagA protein disrupts matrix adhesion of gastric epithelial cells by dephosphorylation of vinculin. Cellular Microbiology 9, 1148-1161.
(Impact: 6.333)
16.) Backert, S., Ziska, E., Brinkmann, V., Zimny-Arndt, U., Fauconnier, A., Jungblut, P.R., Naumann, M., Meyer, T.F. (2000) Translocation of the Helicobacter pylori CagA protein in gastric epithelial cells by a type IV secretion apparatus. Cellular Microbiology 2:
155-164. (Impact: 6.333)
17.) Backert, S., Meißner, K., Börner, T. (1997) Unique features of a mitochondrial rolling circle-plasmid mp1 from the higher plant Chenopodium album (L.), Nucleic Acids Research 25: 582-590. (Impact: 6.317)
18.) Backert, S., Moese, S., Selbach, M., Meyer, T.F. (2001) Phosphorylation of tyrosine 972 of the H. pylori CagA protein is essential for induction of a scattering phenotype in gastric epithelial cells. Molecular Microbiology 42: 631-644. (Impact: 6.203)
19.) Ramarao, N., Gray-Owen, S.D., Backert, S., Meyer, T.F. (2000) Helicobacter pylori inhibits phagocytosis by professional phagocytes involving type IV secretion components.
Molecular Microbiology 37: 1389-1404. (Impact: 6.203)
20.) Backert, S., von Nickisch-Rosenegk, E., Meyer, T.F. (1998) Potential role of two H.
pylori relaxases in DNA transfer? Molecular Microbiology 30: 673-674. (Impact: 6.203)
21.) Backert, S., Müller, E.-C., Jungblut, P.R., Meyer, T.F. (2001) Tyrosine phosphorylation patterns and size modification of the Helicobacter pylori CagA protein after translocation in gastric epithelial cells. Proteomics 1: 608-617. (Impact: 6.088)
22.) Moese, S., Selbach, M., Zimny-Arndt, U., Jungblut, P.R., Meyer, T.F., Backert, S.
(2001) Identification of a tyrosine-phosphorylated 35 kDa C-terminal fragment (p35CagA) of the H. pylori CagA protein in phagocytic cells. Proteomics 1: 618-629. (Impact: 6.088)
23.) Backert, S., Gressmann, H., Kwok, T., König, W., Jungblut, P.R.; Meyer, T.F.
(2005) Gene expression and protein profiling of AGS gastric epithelial cells upon infection with Helicobacter pylori. Proteomics 5: 3902-3918. (Impact: 6.088)
24.) Backert, S., Zimny-Arndt, U., Schmid, M., Kwok, T., Peek, R., König, W., Meyer, T.F., Jungblut, P.R. (2005) Subproteomes of soluble and structure-bound Helicobacter pylori proteins analyzed by two-dimensional gel electrophoresis and mass spectrometry.
Proteomics 5: 1331-1345. (Impact: 6.088)
25.) Selbach, M., Moese, S., Backert, S., Jungblut, P.R., Meyer, T.F.(2004) The Helicobacter pylori CagA protein induces tyrosine dephosphorylation of ezrin. Proteomics 4, 2961-2968. (Impact: 6.088)
26.) Backert, S., Churin, Meyer, T.F. (2002) Helicobacter pylori type IV secretion, host cell signalling and vaccine development. Keio Journal of Medicine 51 Suppl. 2: 6-14.
(Impact: 5.850)
27.) Selbach, M., Moese, S., Hauck, C.R., Meyer, T.F., Backert, S. (2002) Src is the kinase of the Helicobacter pylori CagA protein in vivo and in vitro. Journal of Biological Chemistry 277: 6775-6778. (Impact: 5.808)
28.) Dossumbekova, A., Prinz, C., Mages, J., Lang, R., Kusters, J.G., vanVliet, A.H., Reindl, W.,Backert, S., Aur, D., Schmid, R.M., Rad, R. (2006) Helicobacter pylori HopH (OipA) and bacterial pathogenicity: genetic and functional genomic analysis of hopH gene polymorphisms. Journal of Infectious Diseases 194:1346-1355. (Impact: 5.363)
29.) Zawilak, A., M.C. Durrant, P. Jakimowicz, S. Backert, J. Zakrzewska-Czerwińska (2003) DNA binding specificity of the replication initiator protein, DnaA from Helicobacter pylori. Journal of Molecular Biology 334: 933-947. (Impact: 5.229)
30.) Backert, S., Kunnimalaiyaan, M., Börner, T., Nielsen, B.L. (1998) In vitro replication
of mitochondrial plasmid mp1 from the higher plant Chenopodium album (L.): A remnant of bacterial rolling circle and conjugative plasmids? Journal of Molecular Biology 284:
1005-1015. (Impact: 5.229)
31.) Gelos, M., Backert, S., Klussmann, E., Gratchev, A., Kobalz, U., Mann, B., Moyer, M.P.,Böhm, C., Riecken, E.O., Hanski, C. (1998) Detection of differential gene expression in colorectal cancer cell lines: comparison of three methods. Journal of Molecular Medicine 76: 13. (Impact: 5.157)
32.) Backert, S., Gelos, M., Kobalz, U., Hanski, M.L., Böhm, C., Mann, B., Lövin, N., Gratchev, A., Mansmann, U., Moyer, M.P., Riecken, E.O., Hanski, C. (1999) Differential gene expression in colon carcinoma cells and tissues detected with a cDNA array.
International Journal of Cancer 82: 868-874. (Impact: 4.693)
33.) Blumenthal, B., Hoffmann, C., Aktories, K., Backert, S., Schmidt, G. (2007) The Cytotoxic Necrotizing Factors from Yersinia pseudotuberculosis (CNFY) and from Escherichia coli (CNF1) Bind to Different Cellular Receptors but Take the Same Route to the Cytosol. Infection & Immunity 75, 3344-3353. (Impact: 4.004)
34.) Backert, S.; Schwarz, T., Sommer, Ch., Kirsch, C., Kwok, T., Goebel, U., Lehn, N., Miehlke, S., König, W., Meyer, T.F. (2004) Function of the cag pathogenicity island in Helicobacter pylori isolated from patients with gastritis, peptic ulcer and gastric cancer.
Infection & Immunity 72:1043-1056. (Impact: 4.004)
35.) Moese, S., Selbach, M. Kwok, T., König, W., Meyer, T.F. Backert, S. (2004) Helicobacter pylori induces AGS cell motility and elongation via independent pathways.
Infection & Immunity 72:3646-3649. (Impact: 4.004)
36.) Kwok, T., Backert, S., Schwarz, H., Berger, J., Meyer, T.F. (2002) Specific entry of Helicobacter pylori into cultured gastric epithelial cells via a zipper-like mechanism.
Infection & Immunity 70, 2108-2120. (Impact: 4.004)
37.) Selbach, M., Moese, S., Meyer, T.F., Backert, S. (2002) Functional analysis of Helicobacter pylori cag pathogenicity island reveal VirD4/CagA-dependent and VirD4/CagA-independent mechanisms. Infection & Immunity 70: 665-671. (Impact: 4.004)
38.) Moese, S., Selbach, M., Meyer, T.F., Backert, S. (2002) cag+ Helicobacter pylori induce a homotopic aggregation phenotype in macrophage-like cells by recruitment of ICAM-1 to the cell surface. Infection & Immunity 70: 4687-4691. (Impact: 4.004)
39.) Enarsson, K., Innocenti-Brisslert, M., Backert, S., Quiding-Järbrink, M. (2005) Helicobacter pylori induce transendothelial migration of activated memory T cells.
Infection & Immunity 73: 761-769. (Impact: 4.004)
40.) Backert, S., Lurz, R., Oyarzabal, O.A., Börner, T. (1997) High content, size and distribution of single-stranded DNA in the mitochondria of Chenopodium album (L.), Plant Molecular Biology 33: 1037-1050. (Impact: 3.577)
41.) Bohr, U. R. M., Selgrad, M., Ochman, C., Backert, S. , König, W., Fenske, A., Wex, T., Malfertheimer, P. (2006) Prevalence and spread of enterohepatic Helicobacter in mice reared in a specific pathogen-free facility. J. Clin. Microbiol. 44, 738-742. (Impact: 3.445)
42.) Oyarzabal, O.A., Rad, R., Backert, S. (2007) Conjugative Transfer of a Chromosomally-Encoded Antibiotic Resistance from Helicobacter pylori into Campylobacter jejuni. Journal of Clinical Microbiology 45, 402-408. (Impact: 3.445)
43.) Gieseler, S., König, B., König, W., Backert, S. (2005) Strain-specific expression profiles of virulence genes in Helicobacter pylori during infection of gastric epithelial cells and granulocytes. Microbes & Infection 7: 437-447. (Impact: 3.145)
44.) Backert, S., König, W. (2005) Interplay of bacterial toxins with host defence:
molecular mechanisms of immunomodulatory signaling. International Journal of Medical Microbiology 295: 519-530. (Impact: 2.760)
45.) Oyarzabal, O.A., Backert, S., Nagaraj, M., Miller, R.S., Hussain, K.S., Oyarzabal, E.A. (2007) Efficacy of Supplemented Buffered Peptone Water for the Isolation of Campylobacter jejuni and C. coli from Broiler Retail Products. Journal of Microbiological Methods 69, 129-136. (Impact: 2.442)
46.) Brandt, S., Shafikhani, S., Balachandran, P., Jin, S., Hartig, R., König, W., Backert, S.(2007) Use of a novel co-infection system reveals a role for Rac, Ras, and CrkII phosphorylation in Helicobacter pylori-induced host cell actin cytoskeletal rearrangements.
FEMS Immunology and Med. Microbiology 50, 190-205. (Impact: 2.371)
47.) Backert, S., Börner, T. (2000) Intermediates of replication and recombination in the mitochondria of the higher plant Chenopodium album (L.). Current Genetics 37: 304-314.
(Impact: 2.346)
48.) Backert, S., Lurz, R., Börner, T. (1996) Electron microscopic investigation of mitochondrial DNA from Chenopodium album (L.). Current Genetics 29: 427-436.
(Impact: 2.346)
49.) Backert, S., Dörfel, P., Börner, T. (1995) Investigation of plant organellar DNA by pulsed-field gel electrophoresis. Current Genetics 28: 390-399. (Impact: 2.346)
50.) Krause, M., van Alphen, L., Oyarzabal, O.A., Alter, T. Hänel, I., Schliephake, A., König, W., van Putten, J., Konkel, M., Backert, S. (2007) Expression Patterns and Role of CadF in Campylobacter jejuni and C. coli. FEMS Microbiology Letters 274, 9-16.
(Impact: 2.086)
51.) Brisslert, M., Enarsson, K., Lundin, B.S., Karlsson, A., Kusters J., Svennerholm, A.- M., Backert, S., Quiding-Järbrink, M. (2005) Helicobacter pylori induce neutrophil transendothelial migration: role of the bacterial HP-NAP. FEMS Microbiology Letters 249: 95-103. (Impact: 2.086)
52.) Backert, S. (2000) Strand-switching during rolling circle replication of plasmid-like DNA minicircles in the mitochondria of the higher plant Chenopodium album (L.). Plasmid 43: 166- 170. (Impact: 1.956)
53.) Gratchev, A., Böhm, C., Riede, E., Foss H.D., Hummel, M., Mann, B., Backert, S., Buhr, H.J., Stein, H., Riecken, E.-O., Hanski, C. (1998) Regulation of mucin MUC2 gene expression during colon carcinogenesis. Annals of the New York Academy of Sciences 859:
180-183. (Impact: 1.930)
54.) Potturi-Venkata, L.P., Backert, S., Vieira, S.L., Oyarzabal, O.A. (2007) Evaluation of logistic processing to reduce cross-contamination of commercial broiler carcasses with Campylobacter spp. Journal Food Protection 70, 2549–2554. (Impact: 1.921)
55.) Potturi-Venkata, L.P., Backert, S., Lastovica, A.J., Vieira, S.L., Norton, R.A., Miller, R.S. Pierce, S., Oyarzabal, O.A. (2007) Evaluation of plate media for direct cultivation of Campylobacter species from live broilers. Poultry Science 86, 1304-1311.
(Impact: 1.747)
56.) Oyarzabal, O.A., Wesley, L.V., Harmon, K., Lauerman, L.H., Barbaree, J. M., Backert, S., Tucker, L., Conner, D.E. (1996) Genome variation in Arcobacter subspecies determined by pulsed-field gel electrophoresis. Poultry Science 77. (Impact: 1.747)
57.) Oyarzabal, O.A., Wesley, L.V., Harmon, K., Lauerman, L.H., Barbaree, J. M., Tucker, L., Backert, S., Conner, D.E. (1997) Specific identification of Campylobacter fetus by PCR targeting variable regions of the 16S rRNA, Journal of Veterinary Microbiology 58: 61-71. (Impact: 1.356)
58.) Scissum-Gunn, D.K., Gandhi, M., Backert, S., Nielsen, B.L. (1998) Higher plant mitochondrial DNA can be resolved into distinct populations by field inversion gel electrophoresis. Plant Molecular Biology Reporter 16: 219-229. (Impact: 1.029)
59.) Backert, S., Kwok, T., König, W. (2005) Conjugative plasmid DNA transfer in Helicobacter pylori mediated by chromosomally encoded relaxase and TraG-like proteins.
Microbiology 151(Pt 11): 3493-3503. (Impact: 0.543)
IV Books and Book Chapters
60.) König, W., H. Lauf, U. Arnold, I. Tammer, B. Ghebremedhin, A. Clarici, F.L. Thies, A. Drynda, A. Schmalcz, T. Kwok, R. Arnold, S. Backert, H. Werchau und B. König (2004) Natürliche und adaptive Immunität des Respirationstraktes – Bedeutung mikrobieller Factoren für Infektabwehr und Sensibilisierung, Atemwegs & Lungenkrankungen, 30, Teil 1 Nr. 11/2004, S. 551–568;
Teil2 Nr. 12: 605-620.
61.) König, W., H. Lauf, U. Arnold, I. Tammer, B. Ghebremedhin, A. Clarici, F.L. Thies, A. Drynda, A. Schmalcz, T. Kwok, R. Arnold, S. Backert, H. Werchau und B. König (2004) Natürliche und adaptive Immunität des Respirationstraktes – Bedeutung mikrobieller Factoren für Infektabwehr und Sensibilisierung, Atemwegs &
Lungenkrankungen 30, Teil2 Nr. 12: 605-620.
62.) König, W., H. Lauf, U. Arnold, I. Tammer, B. Ghebremedhin, A. Clarici, F.L. Thies, A. Drynda, A. Schmalcz, T. Kwok, R. Arnold, S. Backert, H. Werchau und B. König
(2005) Natürliche und adaptive Immunität des Respirationstraktes – Bedeutung mikrobieller Factoren für Infektabwehr und Sensibilisierung, Atemwegs &
Lungenkrankungen 31, Teil3 Nr. 4: 188-203.
63.) Backert, S., Fischer, W. (2007) Transport von Proteinen und DNA: Typ IV- Sekretion bei Gram-negativen Bakterien. Naturwissenschaftliche Rundschau 60: 10-17.
64.) Backert, S., Dobrindt, U. (2008) Clinical presentations and pathogenicity of foodborne diseases. In “Food Safety Concepts”, Springer Verlag, edited by O.A.
Oyarzabal, in preparation.
65.) Backert, S., König, W., Arnold, R., König, B. (2006) Induction and modulation of inflammatory networks by bacterial protein toxins. In “The Sourcebook of Bacterial Protein Toxins”, edited by J. E. Alouf, M. R. Popoff, Academic Press, New York. Section V, Chapter 54 : 887-918.
66.) Backert, S., Moyer, P., Böhm, C., Riecken, E.-O., Hanski, C. (1998)Differential gene expression in cell lines derived from colon carcinoma or normal colonic mucosa, Jahrbuch der 8. Wissenschaftswoche FU-Berlin (ISBN-Nr.: 3-9806076-5-8): p. 45-46.
67.) Backert, S. (1996) Untersuchungen zur Struktur und Replikation des Mitochondriengenoms von Chenopodium album (L.), Shaker-Verlag, Aachen (ISBN- Nr.:3-8265-1383-5).
V Case reports and Letters none
