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Immune Responses in Mice after Immunization with Antigens from Different Stages of the Parasite Schistosoma mansoni

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Immune Responses in Mice after Immunization with Antigens from Different Stages of the Parasite Schistosoma mansoni

Hanaa M. Gabera,§, Amany S. Maghrabya,b,*,§, Mohamed Bastawy Ahmedc, Andreas Ruppeld, and Mahmoud M. Bahgata,b,§

a Therapeutical Chemistry Department, Immunology and Infectious Diseases Laboratory, the Center of Excellence for Advanced Sciences, the National Research Center, Dokki, Cairo 12311, Egypt. Fax: 0 02 02-33 37-09 31. E-mail: maghraby_amany@yahoo.com

b Therapeutic Chemistry Department, the National Research Center, Dokki, Cairo 12311, Egypt

c Biochemistry Department, Faculty of Science, Beni Suef University, Beni Suef, Egypt

d Department of Tropical Hygiene and Public Health, Institute of Hygiene,

University of Heidelberg, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany

* Author for correspondence and reprint requests

Z. Naturforsch. 65 c, 289 – 302 (2010); received August 11/December 10, 2009

Mice responses to immunization with Schistosoma mansoni antigens were investigated.

Priming with cercarial antigen preparation (CAP) induced signifi cant (P < 0.05) IgM, IgG, IgG2a, IgG2b, and IgA increases, while booster caused a signifi cant IgG1 increase. A soluble worm antigen preparation (SWAP) caused signifi cant IgG elevation. Priming with soluble egg antigen (SEA) caused signifi cant IgM and IgG2a increases, while booster induced signifi cant IgM, IgG and IgA increases. CAP-immunized mice sera (IMS) recognized CAP peptides ranging from 23 – 78 kDa. SWAP-IMS recognized SWAP peptides ranging from 40 – 75 kDa.

SEA-IMS recognized SEA peptides ranging from 33 – 101 kDa. The cross-reactive peptides among the 3 antigens were identifi ed. CAP caused signifi cant increases in mesenteric lymph nodes (MLNs) CD4,8+, B lymphocytes, CD8+ thymocytes, CD4+ T and B splenocytes. SWAP priming caused signifi cant increases in MLNs CD4,8+ thymocytes and B splenocytes. SWAP booster caused signifi cant increases in MLNs CD8

+ T and B lymphocytes, CD4,8

+ thymocytes and CD4+ T and B splenocytes. SEA caused signifi cant increase in CD4+ T cells.

Key words: Schistosoma mansoni, Ig Classes and Subclasses, Immunofl uoresence, Western Blotting

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