ERK 1/2 / b-actin(% GF-freemedium)pERK1/2 / b-actin(% GF-freemedium)Akt / b-actin(% GF-freemedium)pAktSer473 / b-actin(% GF-freemedium)pAktSer473 / b-actin(% GF-freemedium)

Additional file 8

ERK 1/2 /β-actin (% GF-freemedium) 50

0 100 150 200

GF-freemedium

UKF-NB-3

UKF-NB-3^rVCR¹⁰ UKF-NB-3^rCDDP¹⁰

00

UKF-NB-3^rDOX²⁰ EC medium

0

pERK1/2 /β-actin (% GF-freemedium) 0 100 200 300

GF-freemedium

UKF-NB-3^rVCR¹⁰ UKF-NB-3^rCDDP¹⁰

00

UKF-NB-3^rDOX²⁰ EC medium

Akt / β-actin (% GF-freemedium) 50

0 100 150 200

GF-freemedium

UKF-NB-3^rVCR¹⁰ UKF-NB-3^rCDDP¹⁰

00

UKF-NB-3^rDOX²⁰ EC medium

UKF-NB-3 400

500

UKF-NB-3

pAktSer473 /β-actin (% GF-freemedium) 50

0 100 150 200

GF-freemedium

UKF-NB-3^rVCR¹⁰ UKF-NB-3^rCDDP¹⁰

00

UKF-NB-3^rDOX²⁰ EC medium

UKF-NB-3 pAktSer473 /β-actin (% GF-freemedium) 0 100 200

GF-freemedium

UKF-NB-3^rVCR¹⁰ UKF-NB-3^rCDDP¹⁰

00

UKF-NB-3^rDOX²⁰ EC medium

UKF-NB-3 300

400 500

Additional file 8. Densitometric analysis of Western blot analyses investigating expression of ERK 1/2, phosphorylated ERK 1/2 (pERK 1/2), Akt, Akt phosphorylated at Ser473 (pAkt Ser473), or Akt phosphorylated at Thr308 (pAkt Thr308) in

endothelial cells incubated with 1:1 mixtures of IMDM and supernatants of UKF-NB-3, UKF-NB-3^rVCR¹⁰, UKF-NB-

3^rCDDP¹⁰⁰⁰, or UKF-NB-3^rDOX²⁰cells + 10% FCS in comparison to EC medium and GF-free medium for 24 h. β-actin was used as loading control.